Sleep deprivation reduces the lymphocyte count in a non-obese mouse model of type 1 diabetes mellitus
The objective of the present study was to determine whether sleep deprivation (SD) would promote changes in lymphocyte numbers in a type 1 diabetes model (non-obese diabetic, NOD, mouse strain) and to determine whether SD would affect female and male NOD compared to Swiss mice. The number of lymphoc...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2007-05-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500005 |
| _version_ | 1819178803939770368 |
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| author | F.S. Ruiz M.L. Andersen A. Zager R.C.S. Martins S. Tufik |
| author_facet | F.S. Ruiz M.L. Andersen A. Zager R.C.S. Martins S. Tufik |
| author_sort | F.S. Ruiz |
| collection | DOAJ |
| description | The objective of the present study was to determine whether sleep deprivation (SD) would promote changes in lymphocyte numbers in a type 1 diabetes model (non-obese diabetic, NOD, mouse strain) and to determine whether SD would affect female and male NOD compared to Swiss mice. The number of lymphocytes in peripheral blood after 24 and 96 h of SD (by multiple platform method) or equivalent period of time in home-cage controls was examined prior to the onset of diabetes. SD for 96 h significantly reduced lymphocytes in male Swiss mice compared to control (8.6 ± 2.1 vs 4.1 ± 0.7 10³/µL; P < 0.02). In male NOD animals, 24- and 96-h SD caused a significant decrease of lymphocytes compared to control (4.4 ± 0.3 vs 1.6 ± 0.5; P < 0.001 and 4.4 ± 0.3 vs 0.9 ± 0.1 10³/µL; P < 0.00001, respectively). Both 24- and 96-h SD induced a reduction in the number of lymphocytes in female Swiss (7.5 ± 0.5 vs 4.5 ± 0.5, 4.4 ± 0.6 10³/µL; P < 0.001, respectively) and NOD mice (4 ± 0.6 vs 1.8 ± 0.2, 1.2 ± 0.4 10³/µL; P < 0.01, respectively) compared to the respective controls. Loss of sleep induced lymphopenia in peripheral blood in both genders and strains used. Since many cases of autoimmunity present reduced numbers of lymphocytes and, in this study, it was more evident in the NOD strain, our results suggest that SD should be considered a risk factor in the onset of autoimmune disorders. |
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| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-22T21:48:22Z |
| publishDate | 2007-05-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-b5f0018969a8415d82c8327ff01c8f7a2022-12-21T18:11:26ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2007-05-0140563363710.1590/S0100-879X2007000500005Sleep deprivation reduces the lymphocyte count in a non-obese mouse model of type 1 diabetes mellitusF.S. RuizM.L. AndersenA. ZagerR.C.S. MartinsS. TufikThe objective of the present study was to determine whether sleep deprivation (SD) would promote changes in lymphocyte numbers in a type 1 diabetes model (non-obese diabetic, NOD, mouse strain) and to determine whether SD would affect female and male NOD compared to Swiss mice. The number of lymphocytes in peripheral blood after 24 and 96 h of SD (by multiple platform method) or equivalent period of time in home-cage controls was examined prior to the onset of diabetes. SD for 96 h significantly reduced lymphocytes in male Swiss mice compared to control (8.6 ± 2.1 vs 4.1 ± 0.7 10³/µL; P < 0.02). In male NOD animals, 24- and 96-h SD caused a significant decrease of lymphocytes compared to control (4.4 ± 0.3 vs 1.6 ± 0.5; P < 0.001 and 4.4 ± 0.3 vs 0.9 ± 0.1 10³/µL; P < 0.00001, respectively). Both 24- and 96-h SD induced a reduction in the number of lymphocytes in female Swiss (7.5 ± 0.5 vs 4.5 ± 0.5, 4.4 ± 0.6 10³/µL; P < 0.001, respectively) and NOD mice (4 ± 0.6 vs 1.8 ± 0.2, 1.2 ± 0.4 10³/µL; P < 0.01, respectively) compared to the respective controls. Loss of sleep induced lymphopenia in peripheral blood in both genders and strains used. Since many cases of autoimmunity present reduced numbers of lymphocytes and, in this study, it was more evident in the NOD strain, our results suggest that SD should be considered a risk factor in the onset of autoimmune disorders.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500005Sleep deprivationStressLymphopeniaDiabetesNon-obese diabetic mouseSwiss mice |
| spellingShingle | F.S. Ruiz M.L. Andersen A. Zager R.C.S. Martins S. Tufik Sleep deprivation reduces the lymphocyte count in a non-obese mouse model of type 1 diabetes mellitus Brazilian Journal of Medical and Biological Research Sleep deprivation Stress Lymphopenia Diabetes Non-obese diabetic mouse Swiss mice |
| title | Sleep deprivation reduces the lymphocyte count in a non-obese mouse model of type 1 diabetes mellitus |
| title_full | Sleep deprivation reduces the lymphocyte count in a non-obese mouse model of type 1 diabetes mellitus |
| title_fullStr | Sleep deprivation reduces the lymphocyte count in a non-obese mouse model of type 1 diabetes mellitus |
| title_full_unstemmed | Sleep deprivation reduces the lymphocyte count in a non-obese mouse model of type 1 diabetes mellitus |
| title_short | Sleep deprivation reduces the lymphocyte count in a non-obese mouse model of type 1 diabetes mellitus |
| title_sort | sleep deprivation reduces the lymphocyte count in a non obese mouse model of type 1 diabetes mellitus |
| topic | Sleep deprivation Stress Lymphopenia Diabetes Non-obese diabetic mouse Swiss mice |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500005 |
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