The impact of Beauveria species bioinocula on the soil microbial community structure in organic strawberry plantations
IntroductionThe multifunctionality of microorganisms, including entomopathogenic fungi, represents a feature that could be exploited to support the development, marketing, and application of microbial-based products for plant protection. However, it is likely that this feature could affect the compo...
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| Format: | Article |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1073386/full |
| _version_ | 1797955989852389376 |
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| author | Loredana Canfora Małgorzata Tartanus Małgorzata Tartanus Andrea Manfredini Cezary Tkaczuk Anna Majchrowska-Safaryan Eligio Malusà |
| author_facet | Loredana Canfora Małgorzata Tartanus Małgorzata Tartanus Andrea Manfredini Cezary Tkaczuk Anna Majchrowska-Safaryan Eligio Malusà |
| author_sort | Loredana Canfora |
| collection | DOAJ |
| description | IntroductionThe multifunctionality of microorganisms, including entomopathogenic fungi, represents a feature that could be exploited to support the development, marketing, and application of microbial-based products for plant protection. However, it is likely that this feature could affect the composition and dynamics of the resident soil microorganisms, possibly over a longer period. Therefore, the methodology utilized to evaluate such impact is critical for a reliable assessment. The present study was performed to evaluate the impact of strains of Beauveria brongniartii and Beauveria bassiana on soil bacterial and fungal communities using an approach based on the terminal restriction fragment polymorphism (T-RFLP) analysis.Materials and methodsSoil samples in the vicinity of the root system were collected during a 3-year period, before and after the bioinocula application, in two organic strawberry plantations. Specific primers were used for the amplification of the bacterial 16S rRNA gene and the fungal ITS region of the ribosome.Results and discussionData of the profile analysis from T-RFLP analysis were used to compare the operational taxonomic unit (OTU) occurrence and intensity in the inoculated soil with the uninoculated control. With regard to the impact on the bacterial community, both Beauveria species were not fully consistently affecting their composition across the seasons and fields tested. Nevertheless, some common patterns were pointed out in each field and, sometimes, also among them when considering the time elapsed from the bioinoculum application. The impact was even more inconsistent when analyzing the fungal community. It is thus concluded that the application of the bioinocula induced only a transient and limited effect on the soil microbial community, even though some changes in the structure dynamic and frequency of soil bacterial and fungal OTUs emerged. |
| first_indexed | 2024-04-10T23:42:58Z |
| format | Article |
| id | doaj.art-b5f5ba18639b479a84ae5c42042e9b82 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T23:42:58Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-b5f5ba18639b479a84ae5c42042e9b822023-01-11T06:38:04ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-01-011310.3389/fmicb.2022.10733861073386The impact of Beauveria species bioinocula on the soil microbial community structure in organic strawberry plantationsLoredana Canfora0Małgorzata Tartanus1Małgorzata Tartanus2Andrea Manfredini3Cezary Tkaczuk4Anna Majchrowska-Safaryan5Eligio Malusà6Centre for Agriculture and Environment (CREA-AA), Council for Agricultural Research and Economics, Rome, ItalyCentre for Agriculture and Environment (CREA-AA), Council for Agricultural Research and Economics, Rome, ItalyDepartment of Plant Protection, National Institute of Horticultural Research, Skierniewice, PolandCentre for Agriculture and Environment (CREA-AA), Council for Agricultural Research and Economics, Rome, ItalyInstitute of Agriculture and Horticulture, Siedlce University of Natural Sciences and Humanities, Siedlce, PolandInstitute of Agriculture and Horticulture, Siedlce University of Natural Sciences and Humanities, Siedlce, PolandDepartment of Plant Protection, National Institute of Horticultural Research, Skierniewice, PolandIntroductionThe multifunctionality of microorganisms, including entomopathogenic fungi, represents a feature that could be exploited to support the development, marketing, and application of microbial-based products for plant protection. However, it is likely that this feature could affect the composition and dynamics of the resident soil microorganisms, possibly over a longer period. Therefore, the methodology utilized to evaluate such impact is critical for a reliable assessment. The present study was performed to evaluate the impact of strains of Beauveria brongniartii and Beauveria bassiana on soil bacterial and fungal communities using an approach based on the terminal restriction fragment polymorphism (T-RFLP) analysis.Materials and methodsSoil samples in the vicinity of the root system were collected during a 3-year period, before and after the bioinocula application, in two organic strawberry plantations. Specific primers were used for the amplification of the bacterial 16S rRNA gene and the fungal ITS region of the ribosome.Results and discussionData of the profile analysis from T-RFLP analysis were used to compare the operational taxonomic unit (OTU) occurrence and intensity in the inoculated soil with the uninoculated control. With regard to the impact on the bacterial community, both Beauveria species were not fully consistently affecting their composition across the seasons and fields tested. Nevertheless, some common patterns were pointed out in each field and, sometimes, also among them when considering the time elapsed from the bioinoculum application. The impact was even more inconsistent when analyzing the fungal community. It is thus concluded that the application of the bioinocula induced only a transient and limited effect on the soil microbial community, even though some changes in the structure dynamic and frequency of soil bacterial and fungal OTUs emerged.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1073386/fullBeauveria bassianaBeauveria brongniartiisoil bacteria communitysoil fungal communityT-RFLP |
| spellingShingle | Loredana Canfora Małgorzata Tartanus Małgorzata Tartanus Andrea Manfredini Cezary Tkaczuk Anna Majchrowska-Safaryan Eligio Malusà The impact of Beauveria species bioinocula on the soil microbial community structure in organic strawberry plantations Frontiers in Microbiology Beauveria bassiana Beauveria brongniartii soil bacteria community soil fungal community T-RFLP |
| title | The impact of Beauveria species bioinocula on the soil microbial community structure in organic strawberry plantations |
| title_full | The impact of Beauveria species bioinocula on the soil microbial community structure in organic strawberry plantations |
| title_fullStr | The impact of Beauveria species bioinocula on the soil microbial community structure in organic strawberry plantations |
| title_full_unstemmed | The impact of Beauveria species bioinocula on the soil microbial community structure in organic strawberry plantations |
| title_short | The impact of Beauveria species bioinocula on the soil microbial community structure in organic strawberry plantations |
| title_sort | impact of beauveria species bioinocula on the soil microbial community structure in organic strawberry plantations |
| topic | Beauveria bassiana Beauveria brongniartii soil bacteria community soil fungal community T-RFLP |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1073386/full |
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