Differential responses of rumen and fecal fermentation and microbiota of Liaoning cashmere goats after 2-hydroxy-4-(methylthio) butanoic acid isopropyl ester supplementation
Abstract The 2-hydroxy-4-(methylthio) butanoic acid isopropyl ester (HMBi), a rumen protective methionine, has been extensively studied in dairy cows and beef cattle and has been shown to regulate gastrointestinal microbiota and improve production performance. However, knowledge of the application o...
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Nature Portfolio
2024-04-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-58581-y |
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| author | Zhiqiang Zhong Peiyuan Sun Yuning Zhang Lingyun Li Di Han Xiaoguang Pan Ruiyang Zhang |
| author_facet | Zhiqiang Zhong Peiyuan Sun Yuning Zhang Lingyun Li Di Han Xiaoguang Pan Ruiyang Zhang |
| author_sort | Zhiqiang Zhong |
| collection | DOAJ |
| description | Abstract The 2-hydroxy-4-(methylthio) butanoic acid isopropyl ester (HMBi), a rumen protective methionine, has been extensively studied in dairy cows and beef cattle and has been shown to regulate gastrointestinal microbiota and improve production performance. However, knowledge of the application of HMBi on cashmere goats and the simultaneous study of rumen and hindgut microbiota is still limited. In this study, HMBi supplementation increased the concentration of total serum protein, the production of microbial protein in the rumen and feces, as well as butyrate production in the feces. The results of PCoA and PERMANOVA showed no significant difference between the rumen microbiota, but there was a dramatic difference between the fecal microbiota of the two groups of Cashmere goats after the HMBi supplementation. Specifically, in the rumen, HMBi significantly increased the relative abundance of some fiber-degrading bacteria (such as Fibrobacter) compared with the CON group. In the feces, as well as a similar effect as in the rumen (increasing the relative abundance of some fiber-degrading bacteria, such as Lachnospiraceae FCS020 group and ASV32), HMBi diets also increased the proliferation of butyrate-producing bacteria (including Oscillospiraceae UCG-005 and Christensenellaceae R-7 group). Overall, these results demonstrated that HMBi could regulate the rumen and fecal microbial composition of Liaoning cashmere goats and benefit the host. |
| first_indexed | 2024-04-24T09:54:37Z |
| format | Article |
| id | doaj.art-b5f91972bd2846f7bf5f452759ac00d6 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T09:54:37Z |
| publishDate | 2024-04-01 |
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| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b5f91972bd2846f7bf5f452759ac00d62024-04-14T11:13:02ZengNature PortfolioScientific Reports2045-23222024-04-0114111310.1038/s41598-024-58581-yDifferential responses of rumen and fecal fermentation and microbiota of Liaoning cashmere goats after 2-hydroxy-4-(methylthio) butanoic acid isopropyl ester supplementationZhiqiang Zhong0Peiyuan Sun1Yuning Zhang2Lingyun Li3Di Han4Xiaoguang Pan5Ruiyang Zhang6College of Animal Science and Veterinary Medicine, Shenyang Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Shenyang Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Shenyang Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Shenyang Agricultural UniversityLiaoning Province Modern Agricultural Production Base and Construction Engineering CenterSchool of Artificial Intelligence and Software, Liaoning Petrochemical UniversityCollege of Animal Science and Veterinary Medicine, Shenyang Agricultural UniversityAbstract The 2-hydroxy-4-(methylthio) butanoic acid isopropyl ester (HMBi), a rumen protective methionine, has been extensively studied in dairy cows and beef cattle and has been shown to regulate gastrointestinal microbiota and improve production performance. However, knowledge of the application of HMBi on cashmere goats and the simultaneous study of rumen and hindgut microbiota is still limited. In this study, HMBi supplementation increased the concentration of total serum protein, the production of microbial protein in the rumen and feces, as well as butyrate production in the feces. The results of PCoA and PERMANOVA showed no significant difference between the rumen microbiota, but there was a dramatic difference between the fecal microbiota of the two groups of Cashmere goats after the HMBi supplementation. Specifically, in the rumen, HMBi significantly increased the relative abundance of some fiber-degrading bacteria (such as Fibrobacter) compared with the CON group. In the feces, as well as a similar effect as in the rumen (increasing the relative abundance of some fiber-degrading bacteria, such as Lachnospiraceae FCS020 group and ASV32), HMBi diets also increased the proliferation of butyrate-producing bacteria (including Oscillospiraceae UCG-005 and Christensenellaceae R-7 group). Overall, these results demonstrated that HMBi could regulate the rumen and fecal microbial composition of Liaoning cashmere goats and benefit the host.https://doi.org/10.1038/s41598-024-58581-yRumenFecesHMBiFermentationMicrobial compositionLiaoning cashmere goats |
| spellingShingle | Zhiqiang Zhong Peiyuan Sun Yuning Zhang Lingyun Li Di Han Xiaoguang Pan Ruiyang Zhang Differential responses of rumen and fecal fermentation and microbiota of Liaoning cashmere goats after 2-hydroxy-4-(methylthio) butanoic acid isopropyl ester supplementation Scientific Reports Rumen Feces HMBi Fermentation Microbial composition Liaoning cashmere goats |
| title | Differential responses of rumen and fecal fermentation and microbiota of Liaoning cashmere goats after 2-hydroxy-4-(methylthio) butanoic acid isopropyl ester supplementation |
| title_full | Differential responses of rumen and fecal fermentation and microbiota of Liaoning cashmere goats after 2-hydroxy-4-(methylthio) butanoic acid isopropyl ester supplementation |
| title_fullStr | Differential responses of rumen and fecal fermentation and microbiota of Liaoning cashmere goats after 2-hydroxy-4-(methylthio) butanoic acid isopropyl ester supplementation |
| title_full_unstemmed | Differential responses of rumen and fecal fermentation and microbiota of Liaoning cashmere goats after 2-hydroxy-4-(methylthio) butanoic acid isopropyl ester supplementation |
| title_short | Differential responses of rumen and fecal fermentation and microbiota of Liaoning cashmere goats after 2-hydroxy-4-(methylthio) butanoic acid isopropyl ester supplementation |
| title_sort | differential responses of rumen and fecal fermentation and microbiota of liaoning cashmere goats after 2 hydroxy 4 methylthio butanoic acid isopropyl ester supplementation |
| topic | Rumen Feces HMBi Fermentation Microbial composition Liaoning cashmere goats |
| url | https://doi.org/10.1038/s41598-024-58581-y |
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