Hydroethanolic Extracts of Leaves Possess Antimalarial Effects and Reverses Haematological and Biochemical Pertubation in -infected Mice

The current work investigated the chemical profile, antimalarial potential and capacity of hydroethanolic Senna alata extract (SAE) to reverse hematological and biochemical pertubation in Plasmodium berghei infected mice. Results of the phytochemical analysis revealed the presence of alkaloids, flav...

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Main Authors: Francis O. Atanu, Damilare Rotimi, Omotayo B. Ilesanmi, Jamila S. Al Malki, Gaber E. Batiha, Precious A. Idakwoji
Format: Article
Language:English
Published: SAGE Publishing 2022-08-01
Series:Journal of Evidence-Based Integrative Medicine
Online Access:https://doi.org/10.1177/2515690X221116407
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author Francis O. Atanu
Damilare Rotimi
Omotayo B. Ilesanmi
Jamila S. Al Malki
Gaber E. Batiha
Precious A. Idakwoji
author_facet Francis O. Atanu
Damilare Rotimi
Omotayo B. Ilesanmi
Jamila S. Al Malki
Gaber E. Batiha
Precious A. Idakwoji
author_sort Francis O. Atanu
collection DOAJ
description The current work investigated the chemical profile, antimalarial potential and capacity of hydroethanolic Senna alata extract (SAE) to reverse hematological and biochemical pertubation in Plasmodium berghei infected mice. Results of the phytochemical analysis revealed the presence of alkaloids, flavonoids, phenolics, tannins, terpenoids, saponins, steroids and cardiac glycosides. Total phenolic and flavonoid content was estimated to be 45.29 ± 2.34 mg GAE/g and 25.22 ± 2.26 mg QE/g respectively. In vitro analysis of the extract also confirmed its antioxidant property. Results of the test for prophylaxis of P. berghei indicated that SAE suppressed parasitemia significantly in treated groups in a dose dependent manner when compared with negative control group. Similarly, SAE improved the mean survival time (MST) and packed cell volume (PCV) of infected mice. The test for curative effect showed that SAE significantly suppressed parasitemia to 4.50 ± 1.05% compared to untreated group 29.83 ± 3.49%. Results of liver and kidney functions indices of treated animals indicated that whereas infection with P. berghei caused increase in the levels of AST, ALT, ALP, urea and creatinine, treatment with SAE significantly reversed the perturbation. Similarly, infected mice were dyslipidemic with concomitant increased activity of HMG CoA reductase and decreased activity of antioxidant enzymes with increase in lipid peroxides levels. However, these alterations were significantly reversed by administration of SAE. Results of this study shows that Senna alata possess antimalarial activity and therefore justify the traditional use of plant for the treatment of malaria.
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spelling doaj.art-b5fa8c38ccf64e499f90bff4f01c8ef22022-12-22T04:01:41ZengSAGE PublishingJournal of Evidence-Based Integrative Medicine2515-690X2022-08-012710.1177/2515690X221116407Hydroethanolic Extracts of Leaves Possess Antimalarial Effects and Reverses Haematological and Biochemical Pertubation in -infected MiceFrancis O. Atanu0Damilare Rotimi1Omotayo B. Ilesanmi2Jamila S. Al Malki3Gaber E. Batiha4Precious A. Idakwoji5 Department of Biochemistry, , Kogi State University, Anyigba, Nigeria Department of Biochemistry, Faculty of Pure and Applied Sciences, , Omu-Aran, Kwara State, Nigeria Department of Biochemistry, Faculty of Science, Federal University Otuoke, Yenagoa, Bayelsa State, Nigeria Department of Biology, College of Sciences, , Taif, Saudi Arabia Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, , Damanhour, Albeheira, Egypt Department of Biochemistry, , Kogi State University, Anyigba, NigeriaThe current work investigated the chemical profile, antimalarial potential and capacity of hydroethanolic Senna alata extract (SAE) to reverse hematological and biochemical pertubation in Plasmodium berghei infected mice. Results of the phytochemical analysis revealed the presence of alkaloids, flavonoids, phenolics, tannins, terpenoids, saponins, steroids and cardiac glycosides. Total phenolic and flavonoid content was estimated to be 45.29 ± 2.34 mg GAE/g and 25.22 ± 2.26 mg QE/g respectively. In vitro analysis of the extract also confirmed its antioxidant property. Results of the test for prophylaxis of P. berghei indicated that SAE suppressed parasitemia significantly in treated groups in a dose dependent manner when compared with negative control group. Similarly, SAE improved the mean survival time (MST) and packed cell volume (PCV) of infected mice. The test for curative effect showed that SAE significantly suppressed parasitemia to 4.50 ± 1.05% compared to untreated group 29.83 ± 3.49%. Results of liver and kidney functions indices of treated animals indicated that whereas infection with P. berghei caused increase in the levels of AST, ALT, ALP, urea and creatinine, treatment with SAE significantly reversed the perturbation. Similarly, infected mice were dyslipidemic with concomitant increased activity of HMG CoA reductase and decreased activity of antioxidant enzymes with increase in lipid peroxides levels. However, these alterations were significantly reversed by administration of SAE. Results of this study shows that Senna alata possess antimalarial activity and therefore justify the traditional use of plant for the treatment of malaria.https://doi.org/10.1177/2515690X221116407
spellingShingle Francis O. Atanu
Damilare Rotimi
Omotayo B. Ilesanmi
Jamila S. Al Malki
Gaber E. Batiha
Precious A. Idakwoji
Hydroethanolic Extracts of Leaves Possess Antimalarial Effects and Reverses Haematological and Biochemical Pertubation in -infected Mice
Journal of Evidence-Based Integrative Medicine
title Hydroethanolic Extracts of Leaves Possess Antimalarial Effects and Reverses Haematological and Biochemical Pertubation in -infected Mice
title_full Hydroethanolic Extracts of Leaves Possess Antimalarial Effects and Reverses Haematological and Biochemical Pertubation in -infected Mice
title_fullStr Hydroethanolic Extracts of Leaves Possess Antimalarial Effects and Reverses Haematological and Biochemical Pertubation in -infected Mice
title_full_unstemmed Hydroethanolic Extracts of Leaves Possess Antimalarial Effects and Reverses Haematological and Biochemical Pertubation in -infected Mice
title_short Hydroethanolic Extracts of Leaves Possess Antimalarial Effects and Reverses Haematological and Biochemical Pertubation in -infected Mice
title_sort hydroethanolic extracts of leaves possess antimalarial effects and reverses haematological and biochemical pertubation in infected mice
url https://doi.org/10.1177/2515690X221116407
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