Hand-Portable Miniaturized Liquid Chromatography for the Determination of Chlorogenic Acids in Dietary Supplements
With the explosive growth of the dietary supplements industry, new demands have emerged that cannot be faced with the sophisticated instrumentation available in well-equipped laboratories. In particular, there is a demand for simplified and easy-to-use instruments, capable of providing results in sh...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/12/2408 |
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| author | Camila Soto Henry Daniel Ponce-Rodríguez Jorge Verdú-Andrés Pilar Campíns-Falcó Rosa Herráez-Hernández |
| author_facet | Camila Soto Henry Daniel Ponce-Rodríguez Jorge Verdú-Andrés Pilar Campíns-Falcó Rosa Herráez-Hernández |
| author_sort | Camila Soto |
| collection | DOAJ |
| description | With the explosive growth of the dietary supplements industry, new demands have emerged that cannot be faced with the sophisticated instrumentation available in well-equipped laboratories. In particular, there is a demand for simplified and easy-to-use instruments, capable of providing results in short times of analysis. In this study, a hand-portable miniaturized liquid chromatograph (portable LC) has been tested for the determination of chlorogenic acids (CGAs) in products intended to supplement the diet and elaborated with green coffee extracts. CGAs offer several health benefits due to their antioxidant properties, and an increasing number of dietary supplements are marketed with claimed high contents of these compounds. The results obtained with the proposed portable LC approach have been compared with those obtained with two other miniaturized benchtop liquid chromatography instruments, namely, a capillary liquid chromatograph (capLC) and a nano liquid chromatograph (nanoLC). Although compared with the methods that used the benchtop instruments, the sensitivity attainable was lower, the portable LC instrument provided a comparable analytical performance for the quantification of the main GCAs at low mg g<sup>−1</sup> levels, and it was clearly superior in terms of speed. The proposed portable LC-based method can be applied to assess the content and distribution profile of the predominant CGAs in this kind of dietary supplement. It can be also used to estimate the antioxidant power due to CGAs, as well as their preservation state. |
| first_indexed | 2024-03-09T17:23:19Z |
| format | Article |
| id | doaj.art-b5fcef39937f4e3590910158dc5b4529 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T17:23:19Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-b5fcef39937f4e3590910158dc5b45292023-11-24T12:57:34ZengMDPI AGAntioxidants2076-39212022-12-011112240810.3390/antiox11122408Hand-Portable Miniaturized Liquid Chromatography for the Determination of Chlorogenic Acids in Dietary SupplementsCamila Soto0Henry Daniel Ponce-Rodríguez1Jorge Verdú-Andrés2Pilar Campíns-Falcó3Rosa Herráez-Hernández4MINTOTA Research Group, Departament de Química Analítica, Facultat de Química, Universitat de València, Dr. Moliner 50, 46100 Burjassot, SpainDepartamento de Control Químico, Facultad de Química y Farmacia, Universidad Nacional Autónoma de Honduras, Ciudad Universitaria, Tegucigalpa 11101, HondurasMINTOTA Research Group, Departament de Química Analítica, Facultat de Química, Universitat de València, Dr. Moliner 50, 46100 Burjassot, SpainMINTOTA Research Group, Departament de Química Analítica, Facultat de Química, Universitat de València, Dr. Moliner 50, 46100 Burjassot, SpainMINTOTA Research Group, Departament de Química Analítica, Facultat de Química, Universitat de València, Dr. Moliner 50, 46100 Burjassot, SpainWith the explosive growth of the dietary supplements industry, new demands have emerged that cannot be faced with the sophisticated instrumentation available in well-equipped laboratories. In particular, there is a demand for simplified and easy-to-use instruments, capable of providing results in short times of analysis. In this study, a hand-portable miniaturized liquid chromatograph (portable LC) has been tested for the determination of chlorogenic acids (CGAs) in products intended to supplement the diet and elaborated with green coffee extracts. CGAs offer several health benefits due to their antioxidant properties, and an increasing number of dietary supplements are marketed with claimed high contents of these compounds. The results obtained with the proposed portable LC approach have been compared with those obtained with two other miniaturized benchtop liquid chromatography instruments, namely, a capillary liquid chromatograph (capLC) and a nano liquid chromatograph (nanoLC). Although compared with the methods that used the benchtop instruments, the sensitivity attainable was lower, the portable LC instrument provided a comparable analytical performance for the quantification of the main GCAs at low mg g<sup>−1</sup> levels, and it was clearly superior in terms of speed. The proposed portable LC-based method can be applied to assess the content and distribution profile of the predominant CGAs in this kind of dietary supplement. It can be also used to estimate the antioxidant power due to CGAs, as well as their preservation state.https://www.mdpi.com/2076-3921/11/12/2408dietary supplementsantioxidantschlorogenic acidsminiaturized chromatographyportable liquid chromatography |
| spellingShingle | Camila Soto Henry Daniel Ponce-Rodríguez Jorge Verdú-Andrés Pilar Campíns-Falcó Rosa Herráez-Hernández Hand-Portable Miniaturized Liquid Chromatography for the Determination of Chlorogenic Acids in Dietary Supplements Antioxidants dietary supplements antioxidants chlorogenic acids miniaturized chromatography portable liquid chromatography |
| title | Hand-Portable Miniaturized Liquid Chromatography for the Determination of Chlorogenic Acids in Dietary Supplements |
| title_full | Hand-Portable Miniaturized Liquid Chromatography for the Determination of Chlorogenic Acids in Dietary Supplements |
| title_fullStr | Hand-Portable Miniaturized Liquid Chromatography for the Determination of Chlorogenic Acids in Dietary Supplements |
| title_full_unstemmed | Hand-Portable Miniaturized Liquid Chromatography for the Determination of Chlorogenic Acids in Dietary Supplements |
| title_short | Hand-Portable Miniaturized Liquid Chromatography for the Determination of Chlorogenic Acids in Dietary Supplements |
| title_sort | hand portable miniaturized liquid chromatography for the determination of chlorogenic acids in dietary supplements |
| topic | dietary supplements antioxidants chlorogenic acids miniaturized chromatography portable liquid chromatography |
| url | https://www.mdpi.com/2076-3921/11/12/2408 |
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