The Role of Poly-Herbal Extract in Sodium Chloride-Induced Oxidative Stress and Hyperlipidemia in Male Wistar Rats
Consistent consumption of high salt diet (HSD) has been associated with increased cellular generation of free radicals, which has been implicated in the derangement of some vital organs and etiology of cardiovascular disorders. This study was designed to investigate the combined effect of some commo...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | Medicines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2305-6320/8/6/25 |
| _version_ | 1797531940424777728 |
|---|---|
| author | Olubukola Sinbad Olorunnisola Peter Ifeoluwa Adegbola Bamidele Stephen Ajilore Olayemi Adebola Akintola Olumide Samuel Fadahunsi |
| author_facet | Olubukola Sinbad Olorunnisola Peter Ifeoluwa Adegbola Bamidele Stephen Ajilore Olayemi Adebola Akintola Olumide Samuel Fadahunsi |
| author_sort | Olubukola Sinbad Olorunnisola |
| collection | DOAJ |
| description | Consistent consumption of high salt diet (HSD) has been associated with increased cellular generation of free radicals, which has been implicated in the derangement of some vital organs and etiology of cardiovascular disorders. This study was designed to investigate the combined effect of some commonly employed medicinal plants on serum lipid profile and antioxidant status of aorta, kidney, and liver of high salt diet-fed animals. Out of the total fifty male Wistar rats obtained, fifteen were used for acute toxicity study, while the remaining thirty-five were divided into 5 groups of 7 animals each. Group 1 and 2 animals were fed normal rat chow (NRC) and 16% high salt diet (HSD) only, respectively. Animals in groups 3, 4 and 5 were fed 16% HSD with 800, 400, and 200 mg/kg bw poly-herbal extract (PHE), respectively, once for 28 consecutive days. Serum low-density lipoprotein (LDL), triacylglycerol (TG), total cholesterol (TC) and high-density lipoprotein (HDL), malondialdehyde, nitric oxide, catalase, superoxide dismutase, glutathione peroxidase, glutathione concentration, and activities were assessed in the aorta, kidney, and liver. Poly-herbal extract (<i>p</i> < 0.05) significantly reduced malondialdehyde and nitric oxide concentrations and also increased antioxidant enzymes and glutathione activity. Elevated serum TG, TC, LDL, and TC content in HSD-fed animals were significantly (<i>p</i> < 0.05) reduced to normal in PHE-treated rats while HDL was significantly elevated (<i>p</i> < 0.05) in a concentration-dependent manner in PHE treated animals. Feeding with PHE attenuated high-salt diet imposed derangement in serum lipid profile and antioxidant status in the organs of the experimental rats. |
| first_indexed | 2024-03-10T10:51:58Z |
| format | Article |
| id | doaj.art-b5fff6f0e53c41e38aff9e5f3add5d86 |
| institution | Directory Open Access Journal |
| issn | 2305-6320 |
| language | English |
| last_indexed | 2024-03-10T10:51:58Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medicines |
| spelling | doaj.art-b5fff6f0e53c41e38aff9e5f3add5d862023-11-21T22:11:42ZengMDPI AGMedicines2305-63202021-05-01862510.3390/medicines8060025The Role of Poly-Herbal Extract in Sodium Chloride-Induced Oxidative Stress and Hyperlipidemia in Male Wistar RatsOlubukola Sinbad Olorunnisola0Peter Ifeoluwa Adegbola1Bamidele Stephen Ajilore2Olayemi Adebola Akintola3Olumide Samuel Fadahunsi4Department of Biochemistry, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso P.M.B 4000, Oyo State, NigeriaDepartment of Biochemistry, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso P.M.B 4000, Oyo State, NigeriaDepartment of Medical Biochemistry, Osun State University, Osogbo P.M.B 4494, Osun State, NigeriaDepartment of Science Laboratory and Technology, Faculty of Pure of Applied Sciences, Ladoke Akintola University of Technology, Ogbomoso P.M.B 4000, Oyo State, NigeriaDepartment of Biochemistry, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso P.M.B 4000, Oyo State, NigeriaConsistent consumption of high salt diet (HSD) has been associated with increased cellular generation of free radicals, which has been implicated in the derangement of some vital organs and etiology of cardiovascular disorders. This study was designed to investigate the combined effect of some commonly employed medicinal plants on serum lipid profile and antioxidant status of aorta, kidney, and liver of high salt diet-fed animals. Out of the total fifty male Wistar rats obtained, fifteen were used for acute toxicity study, while the remaining thirty-five were divided into 5 groups of 7 animals each. Group 1 and 2 animals were fed normal rat chow (NRC) and 16% high salt diet (HSD) only, respectively. Animals in groups 3, 4 and 5 were fed 16% HSD with 800, 400, and 200 mg/kg bw poly-herbal extract (PHE), respectively, once for 28 consecutive days. Serum low-density lipoprotein (LDL), triacylglycerol (TG), total cholesterol (TC) and high-density lipoprotein (HDL), malondialdehyde, nitric oxide, catalase, superoxide dismutase, glutathione peroxidase, glutathione concentration, and activities were assessed in the aorta, kidney, and liver. Poly-herbal extract (<i>p</i> < 0.05) significantly reduced malondialdehyde and nitric oxide concentrations and also increased antioxidant enzymes and glutathione activity. Elevated serum TG, TC, LDL, and TC content in HSD-fed animals were significantly (<i>p</i> < 0.05) reduced to normal in PHE-treated rats while HDL was significantly elevated (<i>p</i> < 0.05) in a concentration-dependent manner in PHE treated animals. Feeding with PHE attenuated high-salt diet imposed derangement in serum lipid profile and antioxidant status in the organs of the experimental rats.https://www.mdpi.com/2305-6320/8/6/25sodium chloridepoly-herbalantioxidantslipid profileliverkidney |
| spellingShingle | Olubukola Sinbad Olorunnisola Peter Ifeoluwa Adegbola Bamidele Stephen Ajilore Olayemi Adebola Akintola Olumide Samuel Fadahunsi The Role of Poly-Herbal Extract in Sodium Chloride-Induced Oxidative Stress and Hyperlipidemia in Male Wistar Rats Medicines sodium chloride poly-herbal antioxidants lipid profile liver kidney |
| title | The Role of Poly-Herbal Extract in Sodium Chloride-Induced Oxidative Stress and Hyperlipidemia in Male Wistar Rats |
| title_full | The Role of Poly-Herbal Extract in Sodium Chloride-Induced Oxidative Stress and Hyperlipidemia in Male Wistar Rats |
| title_fullStr | The Role of Poly-Herbal Extract in Sodium Chloride-Induced Oxidative Stress and Hyperlipidemia in Male Wistar Rats |
| title_full_unstemmed | The Role of Poly-Herbal Extract in Sodium Chloride-Induced Oxidative Stress and Hyperlipidemia in Male Wistar Rats |
| title_short | The Role of Poly-Herbal Extract in Sodium Chloride-Induced Oxidative Stress and Hyperlipidemia in Male Wistar Rats |
| title_sort | role of poly herbal extract in sodium chloride induced oxidative stress and hyperlipidemia in male wistar rats |
| topic | sodium chloride poly-herbal antioxidants lipid profile liver kidney |
| url | https://www.mdpi.com/2305-6320/8/6/25 |
| work_keys_str_mv | AT olubukolasinbadolorunnisola theroleofpolyherbalextractinsodiumchlorideinducedoxidativestressandhyperlipidemiainmalewistarrats AT peterifeoluwaadegbola theroleofpolyherbalextractinsodiumchlorideinducedoxidativestressandhyperlipidemiainmalewistarrats AT bamidelestephenajilore theroleofpolyherbalextractinsodiumchlorideinducedoxidativestressandhyperlipidemiainmalewistarrats AT olayemiadebolaakintola theroleofpolyherbalextractinsodiumchlorideinducedoxidativestressandhyperlipidemiainmalewistarrats AT olumidesamuelfadahunsi theroleofpolyherbalextractinsodiumchlorideinducedoxidativestressandhyperlipidemiainmalewistarrats AT olubukolasinbadolorunnisola roleofpolyherbalextractinsodiumchlorideinducedoxidativestressandhyperlipidemiainmalewistarrats AT peterifeoluwaadegbola roleofpolyherbalextractinsodiumchlorideinducedoxidativestressandhyperlipidemiainmalewistarrats AT bamidelestephenajilore roleofpolyherbalextractinsodiumchlorideinducedoxidativestressandhyperlipidemiainmalewistarrats AT olayemiadebolaakintola roleofpolyherbalextractinsodiumchlorideinducedoxidativestressandhyperlipidemiainmalewistarrats AT olumidesamuelfadahunsi roleofpolyherbalextractinsodiumchlorideinducedoxidativestressandhyperlipidemiainmalewistarrats |