Differential expression and correlation of immunoregulation related piRNA in rheumatoid arthritis
BackgroundAlthough PIWI-interacting RNAs (piRNAs) have recently been associated with germline development and many human diseases, their expression pattern and relationship in autoimmune diseases remain indistinct. This study aimed to investigate the presence and correlation of piRNAs in rheumatoid...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1175924/full |
| _version_ | 1827936914599051264 |
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| author | Ruyu Ren Huiling Tan Zhuochun Huang Yuanyi Wang Bin Yang |
| author_facet | Ruyu Ren Huiling Tan Zhuochun Huang Yuanyi Wang Bin Yang |
| author_sort | Ruyu Ren |
| collection | DOAJ |
| description | BackgroundAlthough PIWI-interacting RNAs (piRNAs) have recently been associated with germline development and many human diseases, their expression pattern and relationship in autoimmune diseases remain indistinct. This study aimed to investigate the presence and correlation of piRNAs in rheumatoid arthritis (RA).MethodsWe first analyzed the expression profile of piRNAs using small RNA sequencing in peripheral leukocytes of three new-onset untreated RA patients and three healthy controls (HCs). We then selected piRNAs related to immunoregulation by bioinformatics analysis and verified them in 42 new-onset RA patients and 81 HCs by RT-qPCR. Furthermore, a receiver operating characteristic curve was generated to quantify the diagnostic performance of these piRNAs. A correlation analysis was conducted to observe the link between piRNA expression and RA clinical characteristics.ResultsA total of 15 upregulated and 9 downregulated piRNAs among 1,565 known piRNAs were identified in peripheral leukocytes of RA patients. Dysregulated piRNAs were enriched in numerous pathways related to immunity. After selection and validation, two immunoregulation piRNAs (piR-hsa-27620 and piR-hsa-27124) were significantly elevated in RA patients and have good abilities to distinguish patients from controls, which have the potential to serve as biomarkers. PIWI and other proteins implicated in the piRNA pathway were also associated with RA. |
| first_indexed | 2024-03-13T08:18:00Z |
| format | Article |
| id | doaj.art-b6030040efc944709163a1daf9e37ccf |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-13T08:18:00Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-b6030040efc944709163a1daf9e37ccf2023-05-31T13:52:52ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-05-011410.3389/fimmu.2023.11759241175924Differential expression and correlation of immunoregulation related piRNA in rheumatoid arthritisRuyu Ren0Huiling Tan1Zhuochun Huang2Yuanyi Wang3Bin Yang4Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Spine Surgery, The First Hospital of Jilin University, Changchun, ChinaDepartment of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, ChinaBackgroundAlthough PIWI-interacting RNAs (piRNAs) have recently been associated with germline development and many human diseases, their expression pattern and relationship in autoimmune diseases remain indistinct. This study aimed to investigate the presence and correlation of piRNAs in rheumatoid arthritis (RA).MethodsWe first analyzed the expression profile of piRNAs using small RNA sequencing in peripheral leukocytes of three new-onset untreated RA patients and three healthy controls (HCs). We then selected piRNAs related to immunoregulation by bioinformatics analysis and verified them in 42 new-onset RA patients and 81 HCs by RT-qPCR. Furthermore, a receiver operating characteristic curve was generated to quantify the diagnostic performance of these piRNAs. A correlation analysis was conducted to observe the link between piRNA expression and RA clinical characteristics.ResultsA total of 15 upregulated and 9 downregulated piRNAs among 1,565 known piRNAs were identified in peripheral leukocytes of RA patients. Dysregulated piRNAs were enriched in numerous pathways related to immunity. After selection and validation, two immunoregulation piRNAs (piR-hsa-27620 and piR-hsa-27124) were significantly elevated in RA patients and have good abilities to distinguish patients from controls, which have the potential to serve as biomarkers. PIWI and other proteins implicated in the piRNA pathway were also associated with RA.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1175924/fullpiRNAPIWIrheumatoid arthritisperipheral bloodbiomarker |
| spellingShingle | Ruyu Ren Huiling Tan Zhuochun Huang Yuanyi Wang Bin Yang Differential expression and correlation of immunoregulation related piRNA in rheumatoid arthritis Frontiers in Immunology piRNA PIWI rheumatoid arthritis peripheral blood biomarker |
| title | Differential expression and correlation of immunoregulation related piRNA in rheumatoid arthritis |
| title_full | Differential expression and correlation of immunoregulation related piRNA in rheumatoid arthritis |
| title_fullStr | Differential expression and correlation of immunoregulation related piRNA in rheumatoid arthritis |
| title_full_unstemmed | Differential expression and correlation of immunoregulation related piRNA in rheumatoid arthritis |
| title_short | Differential expression and correlation of immunoregulation related piRNA in rheumatoid arthritis |
| title_sort | differential expression and correlation of immunoregulation related pirna in rheumatoid arthritis |
| topic | piRNA PIWI rheumatoid arthritis peripheral blood biomarker |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1175924/full |
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