Analysis of Chemical Composition and Antioxidant Activities of Water-insoluble and Water-soluble Polysaccharides from Euglena gracilis
Objective: The antioxidant activities and main structural characteristics of water-insoluble and water-soluble polysaccharides of Euglena gracilis were comparatively studied, which provided an experimental basis for the subsequent research of the structure-activity relationship of E. gracilis polysa...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-03-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021060256 |
| _version_ | 1828192004362731520 |
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| author | Yuxi WEN Xiaozhou HUANG Xiaosi LIN |
| author_facet | Yuxi WEN Xiaozhou HUANG Xiaosi LIN |
| author_sort | Yuxi WEN |
| collection | DOAJ |
| description | Objective: The antioxidant activities and main structural characteristics of water-insoluble and water-soluble polysaccharides of Euglena gracilis were comparatively studied, which provided an experimental basis for the subsequent research of the structure-activity relationship of E. gracilis polysaccharides. Methods: The water-insoluble and water-soluble polysaccharides were extracted, separated and purified from E. gracilis. The basic structure of the polysaccharides was analyzed by UV, IR, HPGPC and IC techniques. The antioxidant activities of these polysaccharides were further investigated by measuring scavenging activities against 1,1-Diphenyl-2-picrylhydrazyl free radical (DPPH·) and hydroxyl radical (·OH), and reducing ability, compared with vitamin C (VC). Results: The molecular weights of E. gracilis water-insoluble polysaccharides (EGP-1) and E. gracilis water-soluble polysaccharides (EGP-2A-1 and EGP-2B-1) were 1.01×104, 2.91×106 and 1.70×106 Da, respectively. EGP-1 was a glucopyranose cyclic polysaccharide connected by β-glycosidic bonds, and EGP-2A-1 and EGP-2B-1 were glucopyranose cyclic polysaccharides containing α- and β-glycosidic bonds. The main monosaccharides found in EGP-1 were glucose, mannose, fucose and rhamnose; EGP-2A-1 and EGP-2B-1 were composed of fucose, rhamnose, arabinose, glucosamine, hydrochloride galactose, glucose, xylose, mannose and glucuronic acid. The results of antioxidant activities showed that three-component polysaccharides had antioxidant activity, the order of their antioxidant capacities was VC>EGP-2A-1>EGP-2B-1>EGP-1. Conclusions: Both the water-insoluble and water-soluble polysaccharides isolated from E. gracilis had antioxidant capacity, and the antioxidant activity of water-soluble polysaccharides was stronger than that of water-insoluble polysaccharides, and the EGP-2A-1 was the best. |
| first_indexed | 2024-04-12T08:48:40Z |
| format | Article |
| id | doaj.art-b603f3bb733f4c148ef5d02c411ffe17 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-12T08:48:40Z |
| publishDate | 2022-03-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-b603f3bb733f4c148ef5d02c411ffe172022-12-22T03:39:38ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-03-0143510511310.13386/j.issn1002-0306.20210602562021060256-5Analysis of Chemical Composition and Antioxidant Activities of Water-insoluble and Water-soluble Polysaccharides from Euglena gracilisYuxi WEN0Xiaozhou HUANG1Xiaosi LIN2College of Ocean and Food, Quanzhou Normal University, Quanzhou 362000, ChinaCollege of Ocean and Food, Quanzhou Normal University, Quanzhou 362000, ChinaCollege of Ocean and Food, Quanzhou Normal University, Quanzhou 362000, ChinaObjective: The antioxidant activities and main structural characteristics of water-insoluble and water-soluble polysaccharides of Euglena gracilis were comparatively studied, which provided an experimental basis for the subsequent research of the structure-activity relationship of E. gracilis polysaccharides. Methods: The water-insoluble and water-soluble polysaccharides were extracted, separated and purified from E. gracilis. The basic structure of the polysaccharides was analyzed by UV, IR, HPGPC and IC techniques. The antioxidant activities of these polysaccharides were further investigated by measuring scavenging activities against 1,1-Diphenyl-2-picrylhydrazyl free radical (DPPH·) and hydroxyl radical (·OH), and reducing ability, compared with vitamin C (VC). Results: The molecular weights of E. gracilis water-insoluble polysaccharides (EGP-1) and E. gracilis water-soluble polysaccharides (EGP-2A-1 and EGP-2B-1) were 1.01×104, 2.91×106 and 1.70×106 Da, respectively. EGP-1 was a glucopyranose cyclic polysaccharide connected by β-glycosidic bonds, and EGP-2A-1 and EGP-2B-1 were glucopyranose cyclic polysaccharides containing α- and β-glycosidic bonds. The main monosaccharides found in EGP-1 were glucose, mannose, fucose and rhamnose; EGP-2A-1 and EGP-2B-1 were composed of fucose, rhamnose, arabinose, glucosamine, hydrochloride galactose, glucose, xylose, mannose and glucuronic acid. The results of antioxidant activities showed that three-component polysaccharides had antioxidant activity, the order of their antioxidant capacities was VC>EGP-2A-1>EGP-2B-1>EGP-1. Conclusions: Both the water-insoluble and water-soluble polysaccharides isolated from E. gracilis had antioxidant capacity, and the antioxidant activity of water-soluble polysaccharides was stronger than that of water-insoluble polysaccharides, and the EGP-2A-1 was the best.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021060256euglena gracilispolysaccharidesextractionstructureantioxidant activity |
| spellingShingle | Yuxi WEN Xiaozhou HUANG Xiaosi LIN Analysis of Chemical Composition and Antioxidant Activities of Water-insoluble and Water-soluble Polysaccharides from Euglena gracilis Shipin gongye ke-ji euglena gracilis polysaccharides extraction structure antioxidant activity |
| title | Analysis of Chemical Composition and Antioxidant Activities of Water-insoluble and Water-soluble Polysaccharides from Euglena gracilis |
| title_full | Analysis of Chemical Composition and Antioxidant Activities of Water-insoluble and Water-soluble Polysaccharides from Euglena gracilis |
| title_fullStr | Analysis of Chemical Composition and Antioxidant Activities of Water-insoluble and Water-soluble Polysaccharides from Euglena gracilis |
| title_full_unstemmed | Analysis of Chemical Composition and Antioxidant Activities of Water-insoluble and Water-soluble Polysaccharides from Euglena gracilis |
| title_short | Analysis of Chemical Composition and Antioxidant Activities of Water-insoluble and Water-soluble Polysaccharides from Euglena gracilis |
| title_sort | analysis of chemical composition and antioxidant activities of water insoluble and water soluble polysaccharides from euglena gracilis |
| topic | euglena gracilis polysaccharides extraction structure antioxidant activity |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021060256 |
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