Development and Validation of a Uplc-Qtof-Ms Method for Blood Analysis of Isomeric Amphetamine-Related Drugs
The identification of isomeric drugs is gaining increasing importance in forensics and doping control. Isomers vary in terms of safety, effectiveness, and regulation, particularly for amphetamine-related drugs (ARDs). This study developed and validated a pseudo-isocratic UPLC-qTOF-MS analytical meth...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | Separations |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2297-8739/9/10/285 |
| _version_ | 1827647980302237696 |
|---|---|
| author | Ahmad Alamir James Watterson Ibraheem Attafi |
| author_facet | Ahmad Alamir James Watterson Ibraheem Attafi |
| author_sort | Ahmad Alamir |
| collection | DOAJ |
| description | The identification of isomeric drugs is gaining increasing importance in forensics and doping control. Isomers vary in terms of safety, effectiveness, and regulation, particularly for amphetamine-related drugs (ARDs). This study developed and validated a pseudo-isocratic UPLC-qTOF-MS analytical method for the identification of isomeric Amphetamine-related drugs (ARDs) in blood following mixed-mode solid-phase extraction (MMSPE). The procedure requires 250 μL of blood to achieve a limit of quantification (LOQ) and detection (LOD) of 20 ng/mL for all analytes. In aged animal blood samples, extraction recoveries of 63–90% and matrix effects of 9–21% were observed. Precision and accuracy for all analytes were within 20% and 89–118%, respectively. The analytical method was developed and validated in accordance with the Scientific Working Group for Forensic Toxicology (SWGTOX) Standard. It has acceptable accuracy and precision for use in doping control and forensic toxicology. |
| first_indexed | 2024-03-09T19:30:00Z |
| format | Article |
| id | doaj.art-b606e5f5e5f1484ca20755946366ddf3 |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-09T19:30:00Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Separations |
| spelling | doaj.art-b606e5f5e5f1484ca20755946366ddf32023-11-24T02:32:10ZengMDPI AGSeparations2297-87392022-10-0191028510.3390/separations9100285Development and Validation of a Uplc-Qtof-Ms Method for Blood Analysis of Isomeric Amphetamine-Related DrugsAhmad Alamir0James Watterson1Ibraheem Attafi2Poison Control and Medical Forensic Chemistry Center, Jazan Health Affairs, Ministry of Health, Jazan 45142, Saudi ArabiaDepartment of Forensic Science, Laurentian University, Sudbury, ON P3E 2C6, CanadaPoison Control and Medical Forensic Chemistry Center, Jazan Health Affairs, Ministry of Health, Jazan 45142, Saudi ArabiaThe identification of isomeric drugs is gaining increasing importance in forensics and doping control. Isomers vary in terms of safety, effectiveness, and regulation, particularly for amphetamine-related drugs (ARDs). This study developed and validated a pseudo-isocratic UPLC-qTOF-MS analytical method for the identification of isomeric Amphetamine-related drugs (ARDs) in blood following mixed-mode solid-phase extraction (MMSPE). The procedure requires 250 μL of blood to achieve a limit of quantification (LOQ) and detection (LOD) of 20 ng/mL for all analytes. In aged animal blood samples, extraction recoveries of 63–90% and matrix effects of 9–21% were observed. Precision and accuracy for all analytes were within 20% and 89–118%, respectively. The analytical method was developed and validated in accordance with the Scientific Working Group for Forensic Toxicology (SWGTOX) Standard. It has acceptable accuracy and precision for use in doping control and forensic toxicology.https://www.mdpi.com/2297-8739/9/10/285amphetamine-related drugsforensic toxicologybloodUPLC-qTOF-MSMMSPEvalidation |
| spellingShingle | Ahmad Alamir James Watterson Ibraheem Attafi Development and Validation of a Uplc-Qtof-Ms Method for Blood Analysis of Isomeric Amphetamine-Related Drugs Separations amphetamine-related drugs forensic toxicology blood UPLC-qTOF-MS MMSPE validation |
| title | Development and Validation of a Uplc-Qtof-Ms Method for Blood Analysis of Isomeric Amphetamine-Related Drugs |
| title_full | Development and Validation of a Uplc-Qtof-Ms Method for Blood Analysis of Isomeric Amphetamine-Related Drugs |
| title_fullStr | Development and Validation of a Uplc-Qtof-Ms Method for Blood Analysis of Isomeric Amphetamine-Related Drugs |
| title_full_unstemmed | Development and Validation of a Uplc-Qtof-Ms Method for Blood Analysis of Isomeric Amphetamine-Related Drugs |
| title_short | Development and Validation of a Uplc-Qtof-Ms Method for Blood Analysis of Isomeric Amphetamine-Related Drugs |
| title_sort | development and validation of a uplc qtof ms method for blood analysis of isomeric amphetamine related drugs |
| topic | amphetamine-related drugs forensic toxicology blood UPLC-qTOF-MS MMSPE validation |
| url | https://www.mdpi.com/2297-8739/9/10/285 |
| work_keys_str_mv | AT ahmadalamir developmentandvalidationofauplcqtofmsmethodforbloodanalysisofisomericamphetaminerelateddrugs AT jameswatterson developmentandvalidationofauplcqtofmsmethodforbloodanalysisofisomericamphetaminerelateddrugs AT ibraheemattafi developmentandvalidationofauplcqtofmsmethodforbloodanalysisofisomericamphetaminerelateddrugs |