Differential Regulation of CsrC and CsrB by CRP-cAMP in Salmonella enterica
Post-transcriptional regulation mediated by regulatory small RNAs (sRNAs) has risen as a key player in fine-tuning gene expression in response to environmental stimuli. Here, we show that, in Salmonella enterica, the central metabolic regulator CRP-cAMP differentially regulates the sRNAs CsrB and Cs...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-10-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.570536/full |
| _version_ | 1818162724387946496 |
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| author | Youssef El Mouali Guillem Esteva-Martínez David García-Pedemonte Carlos Balsalobre |
| author_facet | Youssef El Mouali Guillem Esteva-Martínez David García-Pedemonte Carlos Balsalobre |
| author_sort | Youssef El Mouali |
| collection | DOAJ |
| description | Post-transcriptional regulation mediated by regulatory small RNAs (sRNAs) has risen as a key player in fine-tuning gene expression in response to environmental stimuli. Here, we show that, in Salmonella enterica, the central metabolic regulator CRP-cAMP differentially regulates the sRNAs CsrB and CsrC in a growth phase-dependent manner. While CsrB expression remains unchanged during growth, CsrC displays a growth phase-dependent expression profile, being weakly expressed at the logarithmic growth phase and induced upon entry into stationary phase. We show that CRP-cAMP contributes to the expression pattern of CsrC by repressing its expression during the logarithmic growth phase. The CRP-cAMP mediated repression of CsrC is independent of SirA, a known transcriptional CsrB/CsrC activator. We further show that the sRNA Spot 42, which is derepressed in a Δcrp strain, upregulates CsrC during logarithmic growth. We propose a model where the growth-dependent regulation of CsrC is sustained by the CRP-cAMP-mediated repression of Spot 42. Together, our data point toward a differential regulation of the sRNAs CsrB and CsrC in response to environmental stimuli, leading to fine-tuning of gene expression via the sequestration of the RNA-binding protein CsrA. |
| first_indexed | 2024-12-11T16:38:13Z |
| format | Article |
| id | doaj.art-b6099d758ce44bdfa8b634804a59187d |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T16:38:13Z |
| publishDate | 2020-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-b6099d758ce44bdfa8b634804a59187d2022-12-22T00:58:23ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-10-011110.3389/fmicb.2020.570536570536Differential Regulation of CsrC and CsrB by CRP-cAMP in Salmonella entericaYoussef El MoualiGuillem Esteva-MartínezDavid García-PedemonteCarlos BalsalobrePost-transcriptional regulation mediated by regulatory small RNAs (sRNAs) has risen as a key player in fine-tuning gene expression in response to environmental stimuli. Here, we show that, in Salmonella enterica, the central metabolic regulator CRP-cAMP differentially regulates the sRNAs CsrB and CsrC in a growth phase-dependent manner. While CsrB expression remains unchanged during growth, CsrC displays a growth phase-dependent expression profile, being weakly expressed at the logarithmic growth phase and induced upon entry into stationary phase. We show that CRP-cAMP contributes to the expression pattern of CsrC by repressing its expression during the logarithmic growth phase. The CRP-cAMP mediated repression of CsrC is independent of SirA, a known transcriptional CsrB/CsrC activator. We further show that the sRNA Spot 42, which is derepressed in a Δcrp strain, upregulates CsrC during logarithmic growth. We propose a model where the growth-dependent regulation of CsrC is sustained by the CRP-cAMP-mediated repression of Spot 42. Together, our data point toward a differential regulation of the sRNAs CsrB and CsrC in response to environmental stimuli, leading to fine-tuning of gene expression via the sequestration of the RNA-binding protein CsrA.https://www.frontiersin.org/article/10.3389/fmicb.2020.570536/fullCsrCpost-transcriptional regulationsRNACRP-cAMPgrowth phase regulationSpot 42 |
| spellingShingle | Youssef El Mouali Guillem Esteva-Martínez David García-Pedemonte Carlos Balsalobre Differential Regulation of CsrC and CsrB by CRP-cAMP in Salmonella enterica Frontiers in Microbiology CsrC post-transcriptional regulation sRNA CRP-cAMP growth phase regulation Spot 42 |
| title | Differential Regulation of CsrC and CsrB by CRP-cAMP in Salmonella enterica |
| title_full | Differential Regulation of CsrC and CsrB by CRP-cAMP in Salmonella enterica |
| title_fullStr | Differential Regulation of CsrC and CsrB by CRP-cAMP in Salmonella enterica |
| title_full_unstemmed | Differential Regulation of CsrC and CsrB by CRP-cAMP in Salmonella enterica |
| title_short | Differential Regulation of CsrC and CsrB by CRP-cAMP in Salmonella enterica |
| title_sort | differential regulation of csrc and csrb by crp camp in salmonella enterica |
| topic | CsrC post-transcriptional regulation sRNA CRP-cAMP growth phase regulation Spot 42 |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.570536/full |
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