Antioxidant and Anti-aging Mechanism of Wall Broken Rape Pollen
Objective: To investigate the antioxidant and anti-aging effects of wall broken rape pollen on aging model mice induced by D-galactose. Methods: 48 mice were randomly divided into blank control group, aging model group, wall broken rape pollen high (560 mg/kg), medium (280 mg/kg), low (140 mg/kg) gr...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-07-01
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| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021100200 |
| _version_ | 1811224395681628160 |
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| author | Huanle FANG Jie ZHANG Yuwen ZHAO Ningjuan HAN Weidong WANG Yuan LI |
| author_facet | Huanle FANG Jie ZHANG Yuwen ZHAO Ningjuan HAN Weidong WANG Yuan LI |
| author_sort | Huanle FANG |
| collection | DOAJ |
| description | Objective: To investigate the antioxidant and anti-aging effects of wall broken rape pollen on aging model mice induced by D-galactose. Methods: 48 mice were randomly divided into blank control group, aging model group, wall broken rape pollen high (560 mg/kg), medium (280 mg/kg), low (140 mg/kg) group and VC group. The blank control group was intraperitoneally injected with normal saline every day, and the other groups were intraperitoneally injected with D-galactose (120 mg/kg) every day to induce the aging model. At the same time, the drug group was gavaged with high, medium and low doses of broken wall rape pollen suspension and VC solution, weighed every week, and the liver, spleen, kidney and thymus of mice were taken after 6 weeks to weigh the organ mass and calculate the organ index. The contents of superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT) and glutathione peroxidase (GSH-PX) in serum and liver were determined by biochemical method. HE staining was used to observe the morphological changes of liver in aging mice. The expressions of Bax, Sirt1 and p53 proteins in the liver of aging mice were detected by Western Blot. Results: Compared with the aging model group, the medium and high dose groups of broken wall rape pollen could significantly improve the body mass, organ index of thymus, liver, spleen and kidney (P<0.05, P<0.01), significantly increase the contents of SOD, CAT and GSH-Px in serum and liver, reduce the content of MDA (P<0.05, P<0.01), improve hepatocyte swelling, make the expression of the apoptotic protein Bax and pro-aging protein p53 decrease, and the expression of anti-aging protein Sirt1 increase (P<0.05, P<0.01). Conclusion: Broken wall rape pollen can improve the aging of the body by anti-oxidation, anti apoptosis, regulating the content of aging protein. |
| first_indexed | 2024-04-12T08:48:40Z |
| format | Article |
| id | doaj.art-b60a44afad7c4558a5230e7e2e9f16c0 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-12T08:48:40Z |
| publishDate | 2022-07-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-b60a44afad7c4558a5230e7e2e9f16c02022-12-22T03:39:38ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-07-01431437838410.13386/j.issn1002-0306.20211002002021100200-14Antioxidant and Anti-aging Mechanism of Wall Broken Rape PollenHuanle FANG0Jie ZHANG1Yuwen ZHAO2Ningjuan HAN3Weidong WANG4Yuan LI5Medical College of Xi'an Peihua University, Xi'an 710125, ChinaMedical College of Xi'an Peihua University, Xi'an 710125, ChinaMedical College of Xi'an Peihua University, Xi'an 710125, ChinaMedical College of Xi'an Peihua University, Xi'an 710125, ChinaShaanxi Kangtailai Biomedical Engineering Co., Ltd., Xi'an 710065, ChinaMedical College of Xi'an Peihua University, Xi'an 710125, ChinaObjective: To investigate the antioxidant and anti-aging effects of wall broken rape pollen on aging model mice induced by D-galactose. Methods: 48 mice were randomly divided into blank control group, aging model group, wall broken rape pollen high (560 mg/kg), medium (280 mg/kg), low (140 mg/kg) group and VC group. The blank control group was intraperitoneally injected with normal saline every day, and the other groups were intraperitoneally injected with D-galactose (120 mg/kg) every day to induce the aging model. At the same time, the drug group was gavaged with high, medium and low doses of broken wall rape pollen suspension and VC solution, weighed every week, and the liver, spleen, kidney and thymus of mice were taken after 6 weeks to weigh the organ mass and calculate the organ index. The contents of superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT) and glutathione peroxidase (GSH-PX) in serum and liver were determined by biochemical method. HE staining was used to observe the morphological changes of liver in aging mice. The expressions of Bax, Sirt1 and p53 proteins in the liver of aging mice were detected by Western Blot. Results: Compared with the aging model group, the medium and high dose groups of broken wall rape pollen could significantly improve the body mass, organ index of thymus, liver, spleen and kidney (P<0.05, P<0.01), significantly increase the contents of SOD, CAT and GSH-Px in serum and liver, reduce the content of MDA (P<0.05, P<0.01), improve hepatocyte swelling, make the expression of the apoptotic protein Bax and pro-aging protein p53 decrease, and the expression of anti-aging protein Sirt1 increase (P<0.05, P<0.01). Conclusion: Broken wall rape pollen can improve the aging of the body by anti-oxidation, anti apoptosis, regulating the content of aging protein.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021100200broken wall rape pollenantioxidationsirt1/p53 pathwayanti apoptosisanti-aging effect |
| spellingShingle | Huanle FANG Jie ZHANG Yuwen ZHAO Ningjuan HAN Weidong WANG Yuan LI Antioxidant and Anti-aging Mechanism of Wall Broken Rape Pollen Shipin gongye ke-ji broken wall rape pollen antioxidation sirt1/p53 pathway anti apoptosis anti-aging effect |
| title | Antioxidant and Anti-aging Mechanism of Wall Broken Rape Pollen |
| title_full | Antioxidant and Anti-aging Mechanism of Wall Broken Rape Pollen |
| title_fullStr | Antioxidant and Anti-aging Mechanism of Wall Broken Rape Pollen |
| title_full_unstemmed | Antioxidant and Anti-aging Mechanism of Wall Broken Rape Pollen |
| title_short | Antioxidant and Anti-aging Mechanism of Wall Broken Rape Pollen |
| title_sort | antioxidant and anti aging mechanism of wall broken rape pollen |
| topic | broken wall rape pollen antioxidation sirt1/p53 pathway anti apoptosis anti-aging effect |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021100200 |
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