Environmental stressor assessment of hydrocarbonoclastic bacteria biofilms from a marine oil spill
The environmental and economic impact of an oil spill can be significant. Biotechnologies applied during a marine oil spill involve bioaugmentation with immobilised or encapsulated indigenous hydrocarbonoclastic species selected under laboratory conditions to improve degradation rates. The environme...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Biotechnology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X24000079 |
| _version_ | 1797263625732227072 |
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| author | I. Zapata-Peñasco I.A. Avelino-Jiménez J. Mendoza-Pérez M. Vázquez Guevara M. Gutiérrez-Ladrón de Guevara M. Valadez- Martínez L. Hernández-Maya V. Garibay-Febles T. Fregoso-Aguilar J. Fonseca-Campos |
| author_facet | I. Zapata-Peñasco I.A. Avelino-Jiménez J. Mendoza-Pérez M. Vázquez Guevara M. Gutiérrez-Ladrón de Guevara M. Valadez- Martínez L. Hernández-Maya V. Garibay-Febles T. Fregoso-Aguilar J. Fonseca-Campos |
| author_sort | I. Zapata-Peñasco |
| collection | DOAJ |
| description | The environmental and economic impact of an oil spill can be significant. Biotechnologies applied during a marine oil spill involve bioaugmentation with immobilised or encapsulated indigenous hydrocarbonoclastic species selected under laboratory conditions to improve degradation rates. The environmental factors that act as stressors and impact the effectiveness of hydrocarbon removal are one of the challenges associated with these applications. Understanding how native microbes react to environmental stresses is necessary for effective bioaugmentation. Herein, Micrococcus luteus and M. yunnanensis isolated from a marine oil spill mooring system showed hydrocarbonoclastic activity on Maya crude oil in a short time by means of total petroleum hydrocarbons (TPH) at 144 h: M. luteus up to 98.79 % and M. yunnanensis 97.77 % removal. The assessment of Micrococcus biofilms at different temperature (30 °C and 50 °C), pH (5, 6, 7, 8, 9), salinity (30, 50, 60, 70, 80 g/L), and crude oil concentration (1, 5, 15, 25, 35 %) showed different response to the stressors depending on the strain. According to response surface analysis, the main effect was temperature > salinity > hydrocarbon concentration. The hydrocarbonoclastic biofilm architecture was characterised using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Subtle but significant differences were observed: pili in M. luteus by SEM and the topographical differences measured by AFM Power Spectral Density (PSD) analysis, roughness was higher in M. luteus than in M. yunnanensis. In all three domains of life, the Universal Stress Protein (Usp) is crucial for stress adaptation. Herein, the uspA gene expression was analysed in Micrococcus biofilm under environmental stressors. The uspA expression increased up to 2.5-fold in M. luteus biofilms at 30 °C, and 1.3-fold at 50 °C. The highest uspA expression was recorded in M. yunnanensis biofilms at 50 °C with 2.5 and 3-fold with salinities of 50, 60, and 80 g/L at hydrocarbon concentrations of 15, 25, and 35 %. M. yunnanensis biofilms showed greater resilience than M. luteus biofilms when exposed to harsh environmental stressors. M. yunnanensis biofilms were thicker than M. luteus biofilms. Both biofilm responses to environmental stressors through uspA gene expression were consistent with the behaviours observed in the response surface analyses. The uspA gene is a suitable biomarker for assessing environmental stressors of potential microorganisms for bioremediation of marine oil spills and for biosensing the ecophysiological status of native microbiota in a marine petroleum environment. |
| first_indexed | 2024-04-25T00:15:59Z |
| format | Article |
| id | doaj.art-b60df68f69fb40639f49b13e246f5abf |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-04-25T00:15:59Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-b60df68f69fb40639f49b13e246f5abf2024-03-13T04:45:47ZengElsevierBiotechnology Reports2215-017X2024-06-0142e00834Environmental stressor assessment of hydrocarbonoclastic bacteria biofilms from a marine oil spillI. Zapata-Peñasco0I.A. Avelino-Jiménez1J. Mendoza-Pérez2M. Vázquez Guevara3M. Gutiérrez-Ladrón de Guevara4M. Valadez- Martínez5L. Hernández-Maya6V. Garibay-Febles7T. Fregoso-Aguilar8J. Fonseca-Campos9Instituto Mexicano del Petróleo, Eje Central Lázaro Cárdenas Norte 152, Gustavo A. Madero, Ciudad de México, 07730, Mexico; Corresponding author.Instituto Mexicano del Petróleo, Eje Central Lázaro Cárdenas Norte 152, Gustavo A. Madero, Ciudad de México, 07730, MexicoEscuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av. Wilfrido Massieu 399, Nueva Industrial Vallejo, Gustavo A. Madero, 07738, MexicoFacultad de Química, Universidad de Guanajuato, Noria Alta, Guanajuato, 36050, MexicoEscuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av. Wilfrido Massieu 399, Nueva Industrial Vallejo, Gustavo A. Madero, 07738, MexicoInstituto Mexicano del Petróleo, Eje Central Lázaro Cárdenas Norte 152, Gustavo A. Madero, Ciudad de México, 07730, MexicoInstituto Mexicano del Petróleo, Eje Central Lázaro Cárdenas Norte 152, Gustavo A. Madero, Ciudad de México, 07730, MexicoInstituto Mexicano del Petróleo, Eje Central Lázaro Cárdenas Norte 152, Gustavo A. Madero, Ciudad de México, 07730, MexicoEscuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av. Wilfrido Massieu 399, Nueva Industrial Vallejo, Gustavo A. Madero, 07738, MexicoUnidad Profesional Interdisciplinaria en Ingeniería y Tecnologías Avanzadas, Instituto Politécnico Nacional, Av Instituto Politécnico Nacional, Gustavo A. Madero, 07340, MexicoThe environmental and economic impact of an oil spill can be significant. Biotechnologies applied during a marine oil spill involve bioaugmentation with immobilised or encapsulated indigenous hydrocarbonoclastic species selected under laboratory conditions to improve degradation rates. The environmental factors that act as stressors and impact the effectiveness of hydrocarbon removal are one of the challenges associated with these applications. Understanding how native microbes react to environmental stresses is necessary for effective bioaugmentation. Herein, Micrococcus luteus and M. yunnanensis isolated from a marine oil spill mooring system showed hydrocarbonoclastic activity on Maya crude oil in a short time by means of total petroleum hydrocarbons (TPH) at 144 h: M. luteus up to 98.79 % and M. yunnanensis 97.77 % removal. The assessment of Micrococcus biofilms at different temperature (30 °C and 50 °C), pH (5, 6, 7, 8, 9), salinity (30, 50, 60, 70, 80 g/L), and crude oil concentration (1, 5, 15, 25, 35 %) showed different response to the stressors depending on the strain. According to response surface analysis, the main effect was temperature > salinity > hydrocarbon concentration. The hydrocarbonoclastic biofilm architecture was characterised using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Subtle but significant differences were observed: pili in M. luteus by SEM and the topographical differences measured by AFM Power Spectral Density (PSD) analysis, roughness was higher in M. luteus than in M. yunnanensis. In all three domains of life, the Universal Stress Protein (Usp) is crucial for stress adaptation. Herein, the uspA gene expression was analysed in Micrococcus biofilm under environmental stressors. The uspA expression increased up to 2.5-fold in M. luteus biofilms at 30 °C, and 1.3-fold at 50 °C. The highest uspA expression was recorded in M. yunnanensis biofilms at 50 °C with 2.5 and 3-fold with salinities of 50, 60, and 80 g/L at hydrocarbon concentrations of 15, 25, and 35 %. M. yunnanensis biofilms showed greater resilience than M. luteus biofilms when exposed to harsh environmental stressors. M. yunnanensis biofilms were thicker than M. luteus biofilms. Both biofilm responses to environmental stressors through uspA gene expression were consistent with the behaviours observed in the response surface analyses. The uspA gene is a suitable biomarker for assessing environmental stressors of potential microorganisms for bioremediation of marine oil spills and for biosensing the ecophysiological status of native microbiota in a marine petroleum environment.http://www.sciencedirect.com/science/article/pii/S2215017X24000079Marine hydrocarbonoclastic bacteriaBiofilmCrude oil biodegradationOil spillEnvironmental stressoruspA gene expression |
| spellingShingle | I. Zapata-Peñasco I.A. Avelino-Jiménez J. Mendoza-Pérez M. Vázquez Guevara M. Gutiérrez-Ladrón de Guevara M. Valadez- Martínez L. Hernández-Maya V. Garibay-Febles T. Fregoso-Aguilar J. Fonseca-Campos Environmental stressor assessment of hydrocarbonoclastic bacteria biofilms from a marine oil spill Biotechnology Reports Marine hydrocarbonoclastic bacteria Biofilm Crude oil biodegradation Oil spill Environmental stressor uspA gene expression |
| title | Environmental stressor assessment of hydrocarbonoclastic bacteria biofilms from a marine oil spill |
| title_full | Environmental stressor assessment of hydrocarbonoclastic bacteria biofilms from a marine oil spill |
| title_fullStr | Environmental stressor assessment of hydrocarbonoclastic bacteria biofilms from a marine oil spill |
| title_full_unstemmed | Environmental stressor assessment of hydrocarbonoclastic bacteria biofilms from a marine oil spill |
| title_short | Environmental stressor assessment of hydrocarbonoclastic bacteria biofilms from a marine oil spill |
| title_sort | environmental stressor assessment of hydrocarbonoclastic bacteria biofilms from a marine oil spill |
| topic | Marine hydrocarbonoclastic bacteria Biofilm Crude oil biodegradation Oil spill Environmental stressor uspA gene expression |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X24000079 |
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