Serological and molecular characterization of recent lumpy skin disease virus isolates from naturally infected previously vaccinated cattle in Egypt
lumpy skin disease virus (LSDV) was isolated, from naturally infected cattle that have a history of previous vaccination with live attenuated sheep pox virus (SPV) vaccine. The virus was isolated on chorio-allantoic membranes (CAM) of embryonated chicken eggs (ECE) and Madin Darby Bovine Kidney Cell...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, Beni-Suef University
2018-06-01
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| Series: | Journal of Veterinary Medical Research |
| Subjects: | |
| Online Access: | http://www.bsu.edu.eg/bsujournals/Content.aspx?paperId=500&cat_id=1 |
| _version_ | 1818906444762710016 |
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| author | Tamam, S.M. El-Shereif, N.M. Shokier, K.A |
| author_facet | Tamam, S.M. El-Shereif, N.M. Shokier, K.A |
| author_sort | Tamam, S.M. |
| collection | DOAJ |
| description | lumpy skin disease virus (LSDV) was isolated, from naturally infected cattle that have a history of previous vaccination with live attenuated sheep pox virus (SPV) vaccine. The virus was isolated on chorio-allantoic membranes (CAM) of embryonated chicken eggs (ECE) and Madin Darby Bovine Kidney Cells (MDBK) and identified by agar gel precipitation test (AGPT) and immunofluorescent antibody technique (IFA). Characteristic pock lesions and intracyptoplasmic flourescene granules are identified respectively. Molecular characterization using polymerase chain reaction (PCR) using specific primer for G-Protein Coupled Chemokine Receptor Gene of LSDV isolates specific amplified product 554 bp. Sequence analysis revealed tow new isolates of LSDV. |
| first_indexed | 2024-12-19T21:39:20Z |
| format | Article |
| id | doaj.art-b60ea3da757742e69100f1255b107de8 |
| institution | Directory Open Access Journal |
| issn | 2357-0512 2357-0520 |
| language | English |
| last_indexed | 2024-12-19T21:39:20Z |
| publishDate | 2018-06-01 |
| publisher | Faculty of Veterinary Medicine, Beni-Suef University |
| record_format | Article |
| series | Journal of Veterinary Medical Research |
| spelling | doaj.art-b60ea3da757742e69100f1255b107de82022-12-21T20:04:42ZengFaculty of Veterinary Medicine, Beni-Suef UniversityJournal of Veterinary Medical Research2357-05122357-05202018-06-012518091Serological and molecular characterization of recent lumpy skin disease virus isolates from naturally infected previously vaccinated cattle in EgyptTamam, S.M.0El-Shereif, N.M.Shokier, K.AFaculty of Veterinary Medicine. Beni-Suef University, Beni-Suef, Egypt.lumpy skin disease virus (LSDV) was isolated, from naturally infected cattle that have a history of previous vaccination with live attenuated sheep pox virus (SPV) vaccine. The virus was isolated on chorio-allantoic membranes (CAM) of embryonated chicken eggs (ECE) and Madin Darby Bovine Kidney Cells (MDBK) and identified by agar gel precipitation test (AGPT) and immunofluorescent antibody technique (IFA). Characteristic pock lesions and intracyptoplasmic flourescene granules are identified respectively. Molecular characterization using polymerase chain reaction (PCR) using specific primer for G-Protein Coupled Chemokine Receptor Gene of LSDV isolates specific amplified product 554 bp. Sequence analysis revealed tow new isolates of LSDV.http://www.bsu.edu.eg/bsujournals/Content.aspx?paperId=500&cat_id=1lumpy skin diseasecattleEgyptisolation |
| spellingShingle | Tamam, S.M. El-Shereif, N.M. Shokier, K.A Serological and molecular characterization of recent lumpy skin disease virus isolates from naturally infected previously vaccinated cattle in Egypt Journal of Veterinary Medical Research lumpy skin disease cattle Egypt isolation |
| title | Serological and molecular characterization of recent lumpy skin disease virus isolates from naturally infected previously vaccinated cattle in Egypt |
| title_full | Serological and molecular characterization of recent lumpy skin disease virus isolates from naturally infected previously vaccinated cattle in Egypt |
| title_fullStr | Serological and molecular characterization of recent lumpy skin disease virus isolates from naturally infected previously vaccinated cattle in Egypt |
| title_full_unstemmed | Serological and molecular characterization of recent lumpy skin disease virus isolates from naturally infected previously vaccinated cattle in Egypt |
| title_short | Serological and molecular characterization of recent lumpy skin disease virus isolates from naturally infected previously vaccinated cattle in Egypt |
| title_sort | serological and molecular characterization of recent lumpy skin disease virus isolates from naturally infected previously vaccinated cattle in egypt |
| topic | lumpy skin disease cattle Egypt isolation |
| url | http://www.bsu.edu.eg/bsujournals/Content.aspx?paperId=500&cat_id=1 |
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