Identification and analysis of conserved cis-regulatory regions of the MEIS1 gene.
Meis1, a conserved transcription factor of the TALE-homeodomain class, is expressed in a wide variety of tissues during development. Its complex expression pattern is likely to be controlled by an equally complex regulatory landscape. Here we have scanned the Meis1 locus for regulatory elements and...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0033617&type=printable |
| _version_ | 1826801314218115072 |
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| author | José Luis Royo José Bessa Carmen Hidalgo Ana Fernández-Miñán Juan J Tena Yolanda Roncero José Luis Gómez-Skarmeta Fernando Casares |
| author_facet | José Luis Royo José Bessa Carmen Hidalgo Ana Fernández-Miñán Juan J Tena Yolanda Roncero José Luis Gómez-Skarmeta Fernando Casares |
| author_sort | José Luis Royo |
| collection | DOAJ |
| description | Meis1, a conserved transcription factor of the TALE-homeodomain class, is expressed in a wide variety of tissues during development. Its complex expression pattern is likely to be controlled by an equally complex regulatory landscape. Here we have scanned the Meis1 locus for regulatory elements and found 13 non-coding regions, highly conserved between humans and teleost fishes, that have enhancer activity in stable transgenic zebrafish lines. All these regions are syntenic in most vertebrates. The composite expression of all these enhancer elements recapitulate most of Meis1 expression during early embryogenesis, indicating they comprise a basic set of regulatory elements of the Meis1 gene. Using bioinformatic tools, we identify a number of potential binding sites for transcription factors that are compatible with the regulation of these enhancers. Specifically, HHc2:066650, which is expressed in the developing retina and optic tectum, harbors several predicted Pax6 sites. Biochemical, functional and transgenic assays indicate that pax6 genes directly regulate HHc2:066650 activity. |
| first_indexed | 2024-04-13T15:10:16Z |
| format | Article |
| id | doaj.art-b6145c7b413a4e46a042545cccb87c54 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2025-03-17T01:04:54Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b6145c7b413a4e46a042545cccb87c542025-02-19T05:31:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0173e3361710.1371/journal.pone.0033617Identification and analysis of conserved cis-regulatory regions of the MEIS1 gene.José Luis RoyoJosé BessaCarmen HidalgoAna Fernández-MiñánJuan J TenaYolanda RonceroJosé Luis Gómez-SkarmetaFernando CasaresMeis1, a conserved transcription factor of the TALE-homeodomain class, is expressed in a wide variety of tissues during development. Its complex expression pattern is likely to be controlled by an equally complex regulatory landscape. Here we have scanned the Meis1 locus for regulatory elements and found 13 non-coding regions, highly conserved between humans and teleost fishes, that have enhancer activity in stable transgenic zebrafish lines. All these regions are syntenic in most vertebrates. The composite expression of all these enhancer elements recapitulate most of Meis1 expression during early embryogenesis, indicating they comprise a basic set of regulatory elements of the Meis1 gene. Using bioinformatic tools, we identify a number of potential binding sites for transcription factors that are compatible with the regulation of these enhancers. Specifically, HHc2:066650, which is expressed in the developing retina and optic tectum, harbors several predicted Pax6 sites. Biochemical, functional and transgenic assays indicate that pax6 genes directly regulate HHc2:066650 activity.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0033617&type=printable |
| spellingShingle | José Luis Royo José Bessa Carmen Hidalgo Ana Fernández-Miñán Juan J Tena Yolanda Roncero José Luis Gómez-Skarmeta Fernando Casares Identification and analysis of conserved cis-regulatory regions of the MEIS1 gene. PLoS ONE |
| title | Identification and analysis of conserved cis-regulatory regions of the MEIS1 gene. |
| title_full | Identification and analysis of conserved cis-regulatory regions of the MEIS1 gene. |
| title_fullStr | Identification and analysis of conserved cis-regulatory regions of the MEIS1 gene. |
| title_full_unstemmed | Identification and analysis of conserved cis-regulatory regions of the MEIS1 gene. |
| title_short | Identification and analysis of conserved cis-regulatory regions of the MEIS1 gene. |
| title_sort | identification and analysis of conserved cis regulatory regions of the meis1 gene |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0033617&type=printable |
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