Multi-omic characterization of the maize GPI synthesis mutant gwt1 with defects in kernel development
Abstract Background Glycosylphosphatidylinositol (GPI) and GPI-anchored proteins (GAPs) are important for cell wall formation and reproductive development in Arabidopsis. However, monocot counterparts that function in kernel endosperm development have yet to be discovered. Here, we performed a multi...
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| Format: | Article |
| Language: | English |
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BMC
2023-04-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-023-04188-w |
| _version_ | 1827966411605016576 |
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| author | Runmiao Tian Jianjun Jiang Shirong Bo Hui Zhang Xuehai Zhang Sarah Jane Hearne Jihua Tang Dong Ding Zhiyuan Fu |
| author_facet | Runmiao Tian Jianjun Jiang Shirong Bo Hui Zhang Xuehai Zhang Sarah Jane Hearne Jihua Tang Dong Ding Zhiyuan Fu |
| author_sort | Runmiao Tian |
| collection | DOAJ |
| description | Abstract Background Glycosylphosphatidylinositol (GPI) and GPI-anchored proteins (GAPs) are important for cell wall formation and reproductive development in Arabidopsis. However, monocot counterparts that function in kernel endosperm development have yet to be discovered. Here, we performed a multi-omic analysis to explore the function of GPI related genes on kernel development in maize. Results In maize, 48 counterparts of human GPI synthesis and lipid remodeling genes were identified, in which null mutation of the glucosaminyl-phosphatidylinositol O-acyltransferase1 gene, ZmGWT1, caused a kernel mutant (named gwt1) with defects in the basal endosperm transport layer (BETL). We performed plasma membrane (PM) proteomics to characterize the potential GAPs involved in kernel development. In total, 4,981 proteins were successfully identified in 10-DAP gwt1 kernels of mutant and wild-type (WT), including 1,638 membrane-anchored proteins with different posttranslational modifications. Forty-seven of the 256 predicted GAPs were differentially accumulated between gwt1 and WT. Two predicted BETL-specific GAPs (Zm00001d018837 and Zm00001d049834), which kept similar abundance at general proteome but with significantly decreased abundance at membrane proteome in gwt1 were highlighted. Conclusions Our results show the importance of GPI and GAPs for endosperm development and provide candidate genes for further investigation of the regulatory network in which ZmGWT1 participates. |
| first_indexed | 2024-04-09T17:48:31Z |
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| id | doaj.art-b61a14048ae1431ab2c1a65c38b1c3db |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-09T17:48:31Z |
| publishDate | 2023-04-01 |
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| series | BMC Plant Biology |
| spelling | doaj.art-b61a14048ae1431ab2c1a65c38b1c3db2023-04-16T11:10:31ZengBMCBMC Plant Biology1471-22292023-04-0123111510.1186/s12870-023-04188-wMulti-omic characterization of the maize GPI synthesis mutant gwt1 with defects in kernel developmentRunmiao Tian0Jianjun Jiang1Shirong Bo2Hui Zhang3Xuehai Zhang4Sarah Jane Hearne5Jihua Tang6Dong Ding7Zhiyuan Fu8Key Laboratory of Wheat and Maize Crops Science, Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural UniversityKey Laboratory of Wheat and Maize Crops Science, Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural UniversityKey Laboratory of Wheat and Maize Crops Science, Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural UniversityKey Laboratory of Wheat and Maize Crops Science, Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural UniversityKey Laboratory of Wheat and Maize Crops Science, Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural UniversityCIMMYTKey Laboratory of Wheat and Maize Crops Science, Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural UniversityKey Laboratory of Wheat and Maize Crops Science, Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural UniversityKey Laboratory of Wheat and Maize Crops Science, Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural UniversityAbstract Background Glycosylphosphatidylinositol (GPI) and GPI-anchored proteins (GAPs) are important for cell wall formation and reproductive development in Arabidopsis. However, monocot counterparts that function in kernel endosperm development have yet to be discovered. Here, we performed a multi-omic analysis to explore the function of GPI related genes on kernel development in maize. Results In maize, 48 counterparts of human GPI synthesis and lipid remodeling genes were identified, in which null mutation of the glucosaminyl-phosphatidylinositol O-acyltransferase1 gene, ZmGWT1, caused a kernel mutant (named gwt1) with defects in the basal endosperm transport layer (BETL). We performed plasma membrane (PM) proteomics to characterize the potential GAPs involved in kernel development. In total, 4,981 proteins were successfully identified in 10-DAP gwt1 kernels of mutant and wild-type (WT), including 1,638 membrane-anchored proteins with different posttranslational modifications. Forty-seven of the 256 predicted GAPs were differentially accumulated between gwt1 and WT. Two predicted BETL-specific GAPs (Zm00001d018837 and Zm00001d049834), which kept similar abundance at general proteome but with significantly decreased abundance at membrane proteome in gwt1 were highlighted. Conclusions Our results show the importance of GPI and GAPs for endosperm development and provide candidate genes for further investigation of the regulatory network in which ZmGWT1 participates.https://doi.org/10.1186/s12870-023-04188-wMaize endosperm developmentTranscriptomicsProteomicsMembrane proteomicsGPI anchored protein |
| spellingShingle | Runmiao Tian Jianjun Jiang Shirong Bo Hui Zhang Xuehai Zhang Sarah Jane Hearne Jihua Tang Dong Ding Zhiyuan Fu Multi-omic characterization of the maize GPI synthesis mutant gwt1 with defects in kernel development BMC Plant Biology Maize endosperm development Transcriptomics Proteomics Membrane proteomics GPI anchored protein |
| title | Multi-omic characterization of the maize GPI synthesis mutant gwt1 with defects in kernel development |
| title_full | Multi-omic characterization of the maize GPI synthesis mutant gwt1 with defects in kernel development |
| title_fullStr | Multi-omic characterization of the maize GPI synthesis mutant gwt1 with defects in kernel development |
| title_full_unstemmed | Multi-omic characterization of the maize GPI synthesis mutant gwt1 with defects in kernel development |
| title_short | Multi-omic characterization of the maize GPI synthesis mutant gwt1 with defects in kernel development |
| title_sort | multi omic characterization of the maize gpi synthesis mutant gwt1 with defects in kernel development |
| topic | Maize endosperm development Transcriptomics Proteomics Membrane proteomics GPI anchored protein |
| url | https://doi.org/10.1186/s12870-023-04188-w |
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