Immunomodulation of human monocytes following exposure to <it>Lutzomyia intermedia </it>saliva
<p>Abstract</p> <p>Background</p> <p>Sand fly saliva contains potent and complex pharmacologic molecules that are able to modulate the host's hemostatic, inflammatory, and immune systems. In this study, we evaluated the effects of salivary gland sonicate (SGS) of &...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2008-04-01
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| Series: | BMC Immunology |
| Online Access: | http://www.biomedcentral.com/1471-2172/9/12 |
| _version_ | 1828845252195123200 |
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| author | Barral Aldina Miranda José Clarêncio Jorge Costa Dirceu J Menezes Maria Barral-Netto Manoel Brodskyn Cláudia de Oliveira Camila I |
| author_facet | Barral Aldina Miranda José Clarêncio Jorge Costa Dirceu J Menezes Maria Barral-Netto Manoel Brodskyn Cláudia de Oliveira Camila I |
| author_sort | Barral Aldina |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Sand fly saliva contains potent and complex pharmacologic molecules that are able to modulate the host's hemostatic, inflammatory, and immune systems. In this study, we evaluated the effects of salivary gland sonicate (SGS) of <it>Lutzomyia intermedia</it>, the natural vector of <it>Leishmania braziliensis</it>, on monocytes obtained from the peripheral blood mononuclear cells (PBMC) of healthy volunteers. We investigated the effects of sand fly saliva on cytokine production and surface molecule expression of LPS-stimulated human monocytes uninfected or infected with <it>L. braziliensis</it>.</p> <p>Results</p> <p>Pre-treatment of non-infected human monocytes with <it>L. intermedia </it>SGS followed by LPS-stimulation led to a significant decrease in IL-10 production accompanied by a significant increase in CD86, CD80, and HLA-DR expression. Pre-treatment with SGS followed by LPS stimulation and <it>L. braziliensis </it>infection led to a significant increase in TNF-α, IL-6, and IL-8 production without significant alterations in co-stimulatory molecule expression. However, pre-treatment with <it>L. intermedia </it>SGS did not result in significant changes in the infection rate of human monocytes.</p> <p>Conclusion</p> <p>Our data indicate that <it>L. intermedia </it>saliva is able to modulate monocyte response, and, although this modulation is dissociated from enhanced infection with <it>L. braziliensis</it>, it may be associated with successful parasitism.</p> |
| first_indexed | 2024-12-12T21:23:18Z |
| format | Article |
| id | doaj.art-b61c0acb08fd477790b6690f30adfbba |
| institution | Directory Open Access Journal |
| issn | 1471-2172 |
| language | English |
| last_indexed | 2024-12-12T21:23:18Z |
| publishDate | 2008-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Immunology |
| spelling | doaj.art-b61c0acb08fd477790b6690f30adfbba2022-12-22T00:11:31ZengBMCBMC Immunology1471-21722008-04-01911210.1186/1471-2172-9-12Immunomodulation of human monocytes following exposure to <it>Lutzomyia intermedia </it>salivaBarral AldinaMiranda JoséClarêncio JorgeCosta Dirceu JMenezes MariaBarral-Netto ManoelBrodskyn Cláudiade Oliveira Camila I<p>Abstract</p> <p>Background</p> <p>Sand fly saliva contains potent and complex pharmacologic molecules that are able to modulate the host's hemostatic, inflammatory, and immune systems. In this study, we evaluated the effects of salivary gland sonicate (SGS) of <it>Lutzomyia intermedia</it>, the natural vector of <it>Leishmania braziliensis</it>, on monocytes obtained from the peripheral blood mononuclear cells (PBMC) of healthy volunteers. We investigated the effects of sand fly saliva on cytokine production and surface molecule expression of LPS-stimulated human monocytes uninfected or infected with <it>L. braziliensis</it>.</p> <p>Results</p> <p>Pre-treatment of non-infected human monocytes with <it>L. intermedia </it>SGS followed by LPS-stimulation led to a significant decrease in IL-10 production accompanied by a significant increase in CD86, CD80, and HLA-DR expression. Pre-treatment with SGS followed by LPS stimulation and <it>L. braziliensis </it>infection led to a significant increase in TNF-α, IL-6, and IL-8 production without significant alterations in co-stimulatory molecule expression. However, pre-treatment with <it>L. intermedia </it>SGS did not result in significant changes in the infection rate of human monocytes.</p> <p>Conclusion</p> <p>Our data indicate that <it>L. intermedia </it>saliva is able to modulate monocyte response, and, although this modulation is dissociated from enhanced infection with <it>L. braziliensis</it>, it may be associated with successful parasitism.</p>http://www.biomedcentral.com/1471-2172/9/12 |
| spellingShingle | Barral Aldina Miranda José Clarêncio Jorge Costa Dirceu J Menezes Maria Barral-Netto Manoel Brodskyn Cláudia de Oliveira Camila I Immunomodulation of human monocytes following exposure to <it>Lutzomyia intermedia </it>saliva BMC Immunology |
| title | Immunomodulation of human monocytes following exposure to <it>Lutzomyia intermedia </it>saliva |
| title_full | Immunomodulation of human monocytes following exposure to <it>Lutzomyia intermedia </it>saliva |
| title_fullStr | Immunomodulation of human monocytes following exposure to <it>Lutzomyia intermedia </it>saliva |
| title_full_unstemmed | Immunomodulation of human monocytes following exposure to <it>Lutzomyia intermedia </it>saliva |
| title_short | Immunomodulation of human monocytes following exposure to <it>Lutzomyia intermedia </it>saliva |
| title_sort | immunomodulation of human monocytes following exposure to it lutzomyia intermedia it saliva |
| url | http://www.biomedcentral.com/1471-2172/9/12 |
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