Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy
Leukocytes are a part of the immune system that plays an important role in the host’s defense against viral, bacterial, and fungal infections. Among the human leukocytes, two granulocytes, neutrophils (Ne) and eosinophils (EOS) play an important role in the innate immune system. For that purpose, eo...
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MDPI AG
2020-09-01
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| Online Access: | https://www.mdpi.com/2073-4409/9/9/2041 |
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| author | Aleksandra Dorosz Marek Grosicki Jakub Dybas Ewelina Matuszyk Marko Rodewald Tobias Meyer Jürgen Popp Kamilla Malek Malgorzata Baranska |
| author_facet | Aleksandra Dorosz Marek Grosicki Jakub Dybas Ewelina Matuszyk Marko Rodewald Tobias Meyer Jürgen Popp Kamilla Malek Malgorzata Baranska |
| author_sort | Aleksandra Dorosz |
| collection | DOAJ |
| description | Leukocytes are a part of the immune system that plays an important role in the host’s defense against viral, bacterial, and fungal infections. Among the human leukocytes, two granulocytes, neutrophils (Ne) and eosinophils (EOS) play an important role in the innate immune system. For that purpose, eosinophils and neutrophils contain specific granules containing protoporphyrin-type proteins such as eosinophil peroxidase (EPO) and myeloperoxidase (MPO), respectively, which contribute directly to their anti-infection activity. Since both proteins are structurally and functionally different, they could potentially be a marker of both cells’ types. To prove this hypothesis, UV−Vis absorption spectroscopy and Raman imaging were applied to analyze EPO and MPO and their content in leukocytes isolated from the whole blood. Moreover, leukocytes can contain lipidic structures, called lipid bodies (LBs), which are linked to the regulation of immune responses and are considered to be a marker of cell inflammation. In this work, we showed how to determine the number of LBs in two types of granulocytes, EOS and Ne, using fluorescence and coherent anti-Stokes Raman scattering (CARS) microscopy. Spectroscopic differences of EPO and MPO can be used to identify these cells in blood samples, while the detection of LBs can indicate the cell inflammation process. |
| first_indexed | 2024-03-10T16:30:59Z |
| format | Article |
| id | doaj.art-b61c9920e1db4192bb28e4dd15154acf |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T16:30:59Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-b61c9920e1db4192bb28e4dd15154acf2023-11-20T12:52:22ZengMDPI AGCells2073-44092020-09-0199204110.3390/cells9092041Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence MicroscopyAleksandra Dorosz0Marek Grosicki1Jakub Dybas2Ewelina Matuszyk3Marko Rodewald4Tobias Meyer5Jürgen Popp6Kamilla Malek7Malgorzata Baranska8Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, PolandJagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348 Krakow, PolandJagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348 Krakow, PolandJagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348 Krakow, PolandInstitute of Physical Chemistry (IPC) and Abbe Center of Photonics (ACP), Friedrich-Schiller-University, Helmholtzweg 4, 07743 Jena, GermanyLeibniz Institute of Photonic Technology e.V. Member of Leibniz Health Technologies, Jena Albert-Einstein-Str. 9, 07745 Jena, GermanyInstitute of Physical Chemistry (IPC) and Abbe Center of Photonics (ACP), Friedrich-Schiller-University, Helmholtzweg 4, 07743 Jena, GermanyFaculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, PolandFaculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, PolandLeukocytes are a part of the immune system that plays an important role in the host’s defense against viral, bacterial, and fungal infections. Among the human leukocytes, two granulocytes, neutrophils (Ne) and eosinophils (EOS) play an important role in the innate immune system. For that purpose, eosinophils and neutrophils contain specific granules containing protoporphyrin-type proteins such as eosinophil peroxidase (EPO) and myeloperoxidase (MPO), respectively, which contribute directly to their anti-infection activity. Since both proteins are structurally and functionally different, they could potentially be a marker of both cells’ types. To prove this hypothesis, UV−Vis absorption spectroscopy and Raman imaging were applied to analyze EPO and MPO and their content in leukocytes isolated from the whole blood. Moreover, leukocytes can contain lipidic structures, called lipid bodies (LBs), which are linked to the regulation of immune responses and are considered to be a marker of cell inflammation. In this work, we showed how to determine the number of LBs in two types of granulocytes, EOS and Ne, using fluorescence and coherent anti-Stokes Raman scattering (CARS) microscopy. Spectroscopic differences of EPO and MPO can be used to identify these cells in blood samples, while the detection of LBs can indicate the cell inflammation process.https://www.mdpi.com/2073-4409/9/9/2041eosinophilsneutrophilseosinophil peroxidasemyeloperoxidaselipid bodiesRaman microscopy |
| spellingShingle | Aleksandra Dorosz Marek Grosicki Jakub Dybas Ewelina Matuszyk Marko Rodewald Tobias Meyer Jürgen Popp Kamilla Malek Malgorzata Baranska Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy Cells eosinophils neutrophils eosinophil peroxidase myeloperoxidase lipid bodies Raman microscopy |
| title | Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy |
| title_full | Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy |
| title_fullStr | Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy |
| title_full_unstemmed | Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy |
| title_short | Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy |
| title_sort | eosinophils and neutrophils molecular differences revealed by spontaneous raman cars and fluorescence microscopy |
| topic | eosinophils neutrophils eosinophil peroxidase myeloperoxidase lipid bodies Raman microscopy |
| url | https://www.mdpi.com/2073-4409/9/9/2041 |
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