Lysine Inhibits Hemolytic Activity of <i>Staphylococcus aureus</i> and Its Application in Food Model Contaminated with <i>Staphylococcus aureus</i>
Alpha-hemolysin (Hla) is one of the important exotoxins of <i>Staphylococcus aureus</i> (<i>S. aureus)</i> and can be used as a target to reduce the virulence of <i>S. aureus</i>. This study explored the inhibitory effect of Lysine (Lys) on Hla and its application...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/14/12/867 |
| _version_ | 1797455123363921920 |
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| author | Yangli Wan Xiaowen Wang Tianyi Bai Xuting Zheng Liu Yang Qianhong Li Xin Wang |
| author_facet | Yangli Wan Xiaowen Wang Tianyi Bai Xuting Zheng Liu Yang Qianhong Li Xin Wang |
| author_sort | Yangli Wan |
| collection | DOAJ |
| description | Alpha-hemolysin (Hla) is one of the important exotoxins of <i>Staphylococcus aureus</i> (<i>S. aureus)</i> and can be used as a target to reduce the virulence of <i>S. aureus</i>. This study explored the inhibitory effect of Lysine (Lys) on Hla and its application in food safety. Lys significantly inhibited the expression of Hla at sub-inhibitory concentrations and directly interacted with Hla to interfere with its oligomerization and thus significantly inhibited its hemolytic activity. Notably, Lys attenuated <i>S. aureus</i> damage to mouse small intestine and Caco-2 cells and delayed mouse mortality. In the food model, Lys inhibited the expression of Hla of <i>S. aureus</i> and had no significant effect on the sensory score. Moreover, Lys had no obvious damage effect on the main organs of mice, which indicated that Lys has good biocompatibility and has the potential to be used in the food industry as an anti-<i>S. aureus</i> preparation. |
| first_indexed | 2024-03-09T15:46:58Z |
| format | Article |
| id | doaj.art-b6244afb148f44d19bc093a68a9d6500 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-09T15:46:58Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-b6244afb148f44d19bc093a68a9d65002023-11-24T18:27:10ZengMDPI AGToxins2072-66512022-12-01141286710.3390/toxins14120867Lysine Inhibits Hemolytic Activity of <i>Staphylococcus aureus</i> and Its Application in Food Model Contaminated with <i>Staphylococcus aureus</i>Yangli Wan0Xiaowen Wang1Tianyi Bai2Xuting Zheng3Liu Yang4Qianhong Li5Xin Wang6College of Food Science and Engineering, Northwest A&F University, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Xianyang 712100, ChinaAlpha-hemolysin (Hla) is one of the important exotoxins of <i>Staphylococcus aureus</i> (<i>S. aureus)</i> and can be used as a target to reduce the virulence of <i>S. aureus</i>. This study explored the inhibitory effect of Lysine (Lys) on Hla and its application in food safety. Lys significantly inhibited the expression of Hla at sub-inhibitory concentrations and directly interacted with Hla to interfere with its oligomerization and thus significantly inhibited its hemolytic activity. Notably, Lys attenuated <i>S. aureus</i> damage to mouse small intestine and Caco-2 cells and delayed mouse mortality. In the food model, Lys inhibited the expression of Hla of <i>S. aureus</i> and had no significant effect on the sensory score. Moreover, Lys had no obvious damage effect on the main organs of mice, which indicated that Lys has good biocompatibility and has the potential to be used in the food industry as an anti-<i>S. aureus</i> preparation.https://www.mdpi.com/2072-6651/14/12/867food safetyhemolytic activitylysine<i>Staphylococcus aureus</i>α-hemolysin |
| spellingShingle | Yangli Wan Xiaowen Wang Tianyi Bai Xuting Zheng Liu Yang Qianhong Li Xin Wang Lysine Inhibits Hemolytic Activity of <i>Staphylococcus aureus</i> and Its Application in Food Model Contaminated with <i>Staphylococcus aureus</i> Toxins food safety hemolytic activity lysine <i>Staphylococcus aureus</i> α-hemolysin |
| title | Lysine Inhibits Hemolytic Activity of <i>Staphylococcus aureus</i> and Its Application in Food Model Contaminated with <i>Staphylococcus aureus</i> |
| title_full | Lysine Inhibits Hemolytic Activity of <i>Staphylococcus aureus</i> and Its Application in Food Model Contaminated with <i>Staphylococcus aureus</i> |
| title_fullStr | Lysine Inhibits Hemolytic Activity of <i>Staphylococcus aureus</i> and Its Application in Food Model Contaminated with <i>Staphylococcus aureus</i> |
| title_full_unstemmed | Lysine Inhibits Hemolytic Activity of <i>Staphylococcus aureus</i> and Its Application in Food Model Contaminated with <i>Staphylococcus aureus</i> |
| title_short | Lysine Inhibits Hemolytic Activity of <i>Staphylococcus aureus</i> and Its Application in Food Model Contaminated with <i>Staphylococcus aureus</i> |
| title_sort | lysine inhibits hemolytic activity of i staphylococcus aureus i and its application in food model contaminated with i staphylococcus aureus i |
| topic | food safety hemolytic activity lysine <i>Staphylococcus aureus</i> α-hemolysin |
| url | https://www.mdpi.com/2072-6651/14/12/867 |
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