The characteristic analysis of ribosomal protein l12 in haemaphysalis longicornis (acari: ixodidae) ticks
Ribosomal protein L12 (RpL12) plays an important role in ovarian development and engorgement in vertebrates and invertebrates. However, the functional characteristics of RpL12 in ticks are not clear. Here, an open reading frame of the RpL12 gene was cloned from cDNA of Haemaphysalis longicornis. Th...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Kafkas University, Faculty of Veterinary Medicine
2022-08-01
|
| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=2936 |
| _version_ | 1797800795734802432 |
|---|---|
| author | Jin LUO Wenge LIU Qiaoyun REN Xiaokai SONG Ruofeng YAN Guangyuan LIU Xiangrui LI |
| author_facet | Jin LUO Wenge LIU Qiaoyun REN Xiaokai SONG Ruofeng YAN Guangyuan LIU Xiangrui LI |
| author_sort | Jin LUO |
| collection | DOAJ |
| description | Ribosomal protein L12 (RpL12) plays an important role in ovarian development and engorgement in vertebrates and invertebrates.
However, the functional characteristics of RpL12 in ticks are not clear. Here, an open reading frame of the RpL12 gene was cloned from cDNA
of Haemaphysalis longicornis. Th e sequence was analysed, and expression levels were determined in diff erent tissues and developmental stages
using qPCR. To assess the immunization and challenge of ticks, the recombinant protein rRpL12+GST was used in immunological experiments.
Th e results showed high conservation of RpL12 among species and comparisons of the amino acid sequence from H. longicornis. RpL12 was
approximately 60% expressed in the ovary among the examined tissues of unfed adult female H. longicornis, and the expression level of RpL12 in
unfed ticks was signifi cantly lower than that in H. longicornis at the egg and engorged stages. Western blotting showed that rabbit antiserum against
H. longicornis adult ticks recognized RpL12, with an average egg weight of 49.94% and a 17.22% reduction in the engorged weight of adult ticks, but
the mortality increased only 6.00%. Th ese results suggest that RpL12 could be used to generate anti-tick vaccines and provide novel information
on the RpL12 gene of ticks, providing a better understanding of its mechanisms in reproduction and oogenesis. |
| first_indexed | 2024-03-13T04:40:39Z |
| format | Article |
| id | doaj.art-b6283531496b42b8a0b5e2410c532220 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:40:39Z |
| publishDate | 2022-08-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-b6283531496b42b8a0b5e2410c5322202023-06-19T06:15:39ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512022-08-0128557157810.9775/kvfd.2022.275852936The characteristic analysis of ribosomal protein l12 in haemaphysalis longicornis (acari: ixodidae) ticksJin LUO0Wenge LIU1Qiaoyun REN2Xiaokai SONG3Ruofeng YAN4Guangyuan LIU5Xiangrui LI6MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R. CHINAState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu, 730046, P.R. CHINAState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu, 730046, P.R. CHINAMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R. CHINAMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R. CHINAState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu, 730046, P.R. CHINAMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R. CHINARibosomal protein L12 (RpL12) plays an important role in ovarian development and engorgement in vertebrates and invertebrates. However, the functional characteristics of RpL12 in ticks are not clear. Here, an open reading frame of the RpL12 gene was cloned from cDNA of Haemaphysalis longicornis. Th e sequence was analysed, and expression levels were determined in diff erent tissues and developmental stages using qPCR. To assess the immunization and challenge of ticks, the recombinant protein rRpL12+GST was used in immunological experiments. Th e results showed high conservation of RpL12 among species and comparisons of the amino acid sequence from H. longicornis. RpL12 was approximately 60% expressed in the ovary among the examined tissues of unfed adult female H. longicornis, and the expression level of RpL12 in unfed ticks was signifi cantly lower than that in H. longicornis at the egg and engorged stages. Western blotting showed that rabbit antiserum against H. longicornis adult ticks recognized RpL12, with an average egg weight of 49.94% and a 17.22% reduction in the engorged weight of adult ticks, but the mortality increased only 6.00%. Th ese results suggest that RpL12 could be used to generate anti-tick vaccines and provide novel information on the RpL12 gene of ticks, providing a better understanding of its mechanisms in reproduction and oogenesis.https://vetdergikafkas.org/pdf.php?id=2936haemaphysalis longicornisrecombinant proteinribosomal proteinrpl12ticks |
| spellingShingle | Jin LUO Wenge LIU Qiaoyun REN Xiaokai SONG Ruofeng YAN Guangyuan LIU Xiangrui LI The characteristic analysis of ribosomal protein l12 in haemaphysalis longicornis (acari: ixodidae) ticks Kafkas Universitesi Veteriner Fakültesi Dergisi haemaphysalis longicornis recombinant protein ribosomal protein rpl12 ticks |
| title | The characteristic analysis of ribosomal protein l12 in haemaphysalis longicornis (acari: ixodidae) ticks |
| title_full | The characteristic analysis of ribosomal protein l12 in haemaphysalis longicornis (acari: ixodidae) ticks |
| title_fullStr | The characteristic analysis of ribosomal protein l12 in haemaphysalis longicornis (acari: ixodidae) ticks |
| title_full_unstemmed | The characteristic analysis of ribosomal protein l12 in haemaphysalis longicornis (acari: ixodidae) ticks |
| title_short | The characteristic analysis of ribosomal protein l12 in haemaphysalis longicornis (acari: ixodidae) ticks |
| title_sort | characteristic analysis of ribosomal protein l12 in haemaphysalis longicornis acari ixodidae ticks |
| topic | haemaphysalis longicornis recombinant protein ribosomal protein rpl12 ticks |
| url | https://vetdergikafkas.org/pdf.php?id=2936 |
| work_keys_str_mv | AT jinluo thecharacteristicanalysisofribosomalproteinl12inhaemaphysalislongicornisacariixodidaeticks AT wengeliu thecharacteristicanalysisofribosomalproteinl12inhaemaphysalislongicornisacariixodidaeticks AT qiaoyunren thecharacteristicanalysisofribosomalproteinl12inhaemaphysalislongicornisacariixodidaeticks AT xiaokaisong thecharacteristicanalysisofribosomalproteinl12inhaemaphysalislongicornisacariixodidaeticks AT ruofengyan thecharacteristicanalysisofribosomalproteinl12inhaemaphysalislongicornisacariixodidaeticks AT guangyuanliu thecharacteristicanalysisofribosomalproteinl12inhaemaphysalislongicornisacariixodidaeticks AT xiangruili thecharacteristicanalysisofribosomalproteinl12inhaemaphysalislongicornisacariixodidaeticks AT jinluo characteristicanalysisofribosomalproteinl12inhaemaphysalislongicornisacariixodidaeticks AT wengeliu characteristicanalysisofribosomalproteinl12inhaemaphysalislongicornisacariixodidaeticks AT qiaoyunren characteristicanalysisofribosomalproteinl12inhaemaphysalislongicornisacariixodidaeticks AT xiaokaisong characteristicanalysisofribosomalproteinl12inhaemaphysalislongicornisacariixodidaeticks AT ruofengyan characteristicanalysisofribosomalproteinl12inhaemaphysalislongicornisacariixodidaeticks AT guangyuanliu characteristicanalysisofribosomalproteinl12inhaemaphysalislongicornisacariixodidaeticks AT xiangruili characteristicanalysisofribosomalproteinl12inhaemaphysalislongicornisacariixodidaeticks |