Effects of Aging and Anatomic Location on Gene Expression in Human Retina
Objective: To determine the effects of age and topographic location on gene expression in human neural retina.Methods: Macular and peripheral neural retina RNA were isolated from human donor eyes for DNA microarray and quantitative RT-PCR analyses.Results: Total RNA integrity from human donors was p...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2012-05-01
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| Series: | Frontiers in Aging Neuroscience |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnagi.2012.00008/full |
| _version_ | 1818139488612777984 |
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| author | Hui eCai Mark A Fields Risa eHoshino Lucian V Del Priore |
| author_facet | Hui eCai Mark A Fields Risa eHoshino Lucian V Del Priore |
| author_sort | Hui eCai |
| collection | DOAJ |
| description | Objective: To determine the effects of age and topographic location on gene expression in human neural retina.Methods: Macular and peripheral neural retina RNA were isolated from human donor eyes for DNA microarray and quantitative RT-PCR analyses.Results: Total RNA integrity from human donors was preserved. Hierarchical clustering analysis demonstrates that the gene expression profiles of young, old, macula and peripheral retina cluster into four distinct groups. Genes which are highly expressed in macular, peripheral, young or old retina were identified, including inhibitors of Wnt Signaling Pathway (DKK1, FZD10 and SFRP2) which are preferably expressed in the periphery. Conclusions: The transcriptome of the human retina is affected by age and topographic location. Wnt pathway inhibitors in the periphery may maintain peripheral retinal cells in an undifferentiated state. Understanding the effects of age and topographic location on gene expression may lead to the development of new therapeutic interventions for age-related eye diseases. |
| first_indexed | 2024-12-11T10:28:53Z |
| format | Article |
| id | doaj.art-b62d08af53944d86868c7447f675da30 |
| institution | Directory Open Access Journal |
| issn | 1663-4365 |
| language | English |
| last_indexed | 2024-12-11T10:28:53Z |
| publishDate | 2012-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Aging Neuroscience |
| spelling | doaj.art-b62d08af53944d86868c7447f675da302022-12-22T01:11:02ZengFrontiers Media S.A.Frontiers in Aging Neuroscience1663-43652012-05-01410.3389/fnagi.2012.0000812018Effects of Aging and Anatomic Location on Gene Expression in Human RetinaHui eCai0Mark A Fields1Risa eHoshino2Lucian V Del Priore3Columbia University Medical CenterColumbia University Medical CenterColumbia University Medical CenterColumbia University Medical CenterObjective: To determine the effects of age and topographic location on gene expression in human neural retina.Methods: Macular and peripheral neural retina RNA were isolated from human donor eyes for DNA microarray and quantitative RT-PCR analyses.Results: Total RNA integrity from human donors was preserved. Hierarchical clustering analysis demonstrates that the gene expression profiles of young, old, macula and peripheral retina cluster into four distinct groups. Genes which are highly expressed in macular, peripheral, young or old retina were identified, including inhibitors of Wnt Signaling Pathway (DKK1, FZD10 and SFRP2) which are preferably expressed in the periphery. Conclusions: The transcriptome of the human retina is affected by age and topographic location. Wnt pathway inhibitors in the periphery may maintain peripheral retinal cells in an undifferentiated state. Understanding the effects of age and topographic location on gene expression may lead to the development of new therapeutic interventions for age-related eye diseases.http://journal.frontiersin.org/Journal/10.3389/fnagi.2012.00008/fullAgingGene ExpressionWnt Signaling PathwayHuman retinamaculaperipheral |
| spellingShingle | Hui eCai Mark A Fields Risa eHoshino Lucian V Del Priore Effects of Aging and Anatomic Location on Gene Expression in Human Retina Frontiers in Aging Neuroscience Aging Gene Expression Wnt Signaling Pathway Human retina macula peripheral |
| title | Effects of Aging and Anatomic Location on Gene Expression in Human Retina |
| title_full | Effects of Aging and Anatomic Location on Gene Expression in Human Retina |
| title_fullStr | Effects of Aging and Anatomic Location on Gene Expression in Human Retina |
| title_full_unstemmed | Effects of Aging and Anatomic Location on Gene Expression in Human Retina |
| title_short | Effects of Aging and Anatomic Location on Gene Expression in Human Retina |
| title_sort | effects of aging and anatomic location on gene expression in human retina |
| topic | Aging Gene Expression Wnt Signaling Pathway Human retina macula peripheral |
| url | http://journal.frontiersin.org/Journal/10.3389/fnagi.2012.00008/full |
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