Effects of heterologous genome microinjection on the enlargement of Enterococcus faecalis protoplasts
The lactic acid bacterium Enterococcus faecalis genomic DNA and seven phylogenetically distant bacterial genomic DNAs were microinjected into 126 enlarged protoplasts of E. faecalis. After the microinjection, a time-lapse observation was performed on how the cells enlarged. Most cells did not stop e...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Current Research in Microbial Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666517422000013 |
| _version_ | 1811178740020936704 |
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| author | Sawako Takahashi Hiromi Nishida |
| author_facet | Sawako Takahashi Hiromi Nishida |
| author_sort | Sawako Takahashi |
| collection | DOAJ |
| description | The lactic acid bacterium Enterococcus faecalis genomic DNA and seven phylogenetically distant bacterial genomic DNAs were microinjected into 126 enlarged protoplasts of E. faecalis. After the microinjection, a time-lapse observation was performed on how the cells enlarged. Most cells did not stop enlarging. The enlargement patterns were compared with the enlargement of E. faecalis protoplasts not treated by microinjection (control). They were clustered into three groups, with different levels and speeds of protoplast enlargement. The statistical analyses showed that the protoplasts injected by E. faecalis and four of the seven phylogenetically different bacterial genomic DNAs had enlargement patterns significantly different from those of the control. Thus, injected genomic DNAs affected the protoplast enlargement. Most of the affected cells, including the E. faecalis genome, had weakened enlargement. |
| first_indexed | 2024-04-11T06:24:27Z |
| format | Article |
| id | doaj.art-b6338e6c74d043949935a87c41851b7c |
| institution | Directory Open Access Journal |
| issn | 2666-5174 |
| language | English |
| last_indexed | 2024-04-11T06:24:27Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Microbial Sciences |
| spelling | doaj.art-b6338e6c74d043949935a87c41851b7c2022-12-22T04:40:28ZengElsevierCurrent Research in Microbial Sciences2666-51742022-01-013100104Effects of heterologous genome microinjection on the enlargement of Enterococcus faecalis protoplastsSawako Takahashi0Hiromi Nishida1Graduate School of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, JapanCorresponding author.; Graduate School of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, JapanThe lactic acid bacterium Enterococcus faecalis genomic DNA and seven phylogenetically distant bacterial genomic DNAs were microinjected into 126 enlarged protoplasts of E. faecalis. After the microinjection, a time-lapse observation was performed on how the cells enlarged. Most cells did not stop enlarging. The enlargement patterns were compared with the enlargement of E. faecalis protoplasts not treated by microinjection (control). They were clustered into three groups, with different levels and speeds of protoplast enlargement. The statistical analyses showed that the protoplasts injected by E. faecalis and four of the seven phylogenetically different bacterial genomic DNAs had enlargement patterns significantly different from those of the control. Thus, injected genomic DNAs affected the protoplast enlargement. Most of the affected cells, including the E. faecalis genome, had weakened enlargement.http://www.sciencedirect.com/science/article/pii/S2666517422000013Bacterial chromosomeEnterococcus faecalisGenome stabilityMicroinjectionProtoplast enlargement |
| spellingShingle | Sawako Takahashi Hiromi Nishida Effects of heterologous genome microinjection on the enlargement of Enterococcus faecalis protoplasts Current Research in Microbial Sciences Bacterial chromosome Enterococcus faecalis Genome stability Microinjection Protoplast enlargement |
| title | Effects of heterologous genome microinjection on the enlargement of Enterococcus faecalis protoplasts |
| title_full | Effects of heterologous genome microinjection on the enlargement of Enterococcus faecalis protoplasts |
| title_fullStr | Effects of heterologous genome microinjection on the enlargement of Enterococcus faecalis protoplasts |
| title_full_unstemmed | Effects of heterologous genome microinjection on the enlargement of Enterococcus faecalis protoplasts |
| title_short | Effects of heterologous genome microinjection on the enlargement of Enterococcus faecalis protoplasts |
| title_sort | effects of heterologous genome microinjection on the enlargement of enterococcus faecalis protoplasts |
| topic | Bacterial chromosome Enterococcus faecalis Genome stability Microinjection Protoplast enlargement |
| url | http://www.sciencedirect.com/science/article/pii/S2666517422000013 |
| work_keys_str_mv | AT sawakotakahashi effectsofheterologousgenomemicroinjectionontheenlargementofenterococcusfaecalisprotoplasts AT hirominishida effectsofheterologousgenomemicroinjectionontheenlargementofenterococcusfaecalisprotoplasts |