The effect of Ca-Dobesilate over renal scar formation in an experimental pyelonephritis model
Purpose: This study was conducted to evaluate the effects of the drug: Ca-Dobesilate (CaD) which has been in common use in venous insufficiency treatment; on renal scarring and expressions of transforming growth factor beta1 (TGFb1), basic fibroblast growth factor (bFGF) and hepatocyte growth factor...
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| Format: | Article |
| Language: | English |
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Cukurova University
2020-12-01
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| Series: | Cukurova Medical Journal |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/1244164 |
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| author | Gülfiliz Gönlüşen Aytül Noyan Ali Anarat Behcet Simsek Aysun Karabay Bayazıt |
| author_facet | Gülfiliz Gönlüşen Aytül Noyan Ali Anarat Behcet Simsek Aysun Karabay Bayazıt |
| author_sort | Gülfiliz Gönlüşen |
| collection | DOAJ |
| description | Purpose: This study was conducted to evaluate the effects of the drug: Ca-Dobesilate (CaD) which has been in common use in venous insufficiency treatment; on renal scarring and expressions of transforming growth factor beta1 (TGFb1), basic fibroblast growth factor (bFGF) and hepatocyte growth factor-beta (HGF-beta) in a rat pyelonephritis model.
Materialw and Methods: Eight pyelonephritis groups, each constituting of 7 rats were developed as no treatment - ciprofloxacin – ciprofloxacin and CaD administered groups; following injecting E Coli (ATCC 25922) into kidney. No treatment given rat groups were sacrificed following 24h, 72 h, 14d and 28d from bacterial seeding respectively. Rats from treatment groups were sacrificed after 14d and 28d accordingly. Diagnoses of pyelonephritis and fibrosis, TGFb, bFGF and HGF-beta were scored semiquantitatively by immunohistochemical staining.
Results: The extent of pyelonephritis and fibrosis was lower in rats treated with ciprofloxacin and CaD compared to sole ciprofloxacin and no treatment administered counterparts among groups terminated after 2wks following bacterial inoculation. However, CaD effect on pyelonephritis and fibrosis scores did not persist after treatment was discontinued.
Conclusion: CaD might alleviate pyelonephritis and scarring, depending on dosage and treatment period and further studies are needed to determine optimum treatment dose and duration. |
| first_indexed | 2024-04-10T14:02:27Z |
| format | Article |
| id | doaj.art-b638ea56f4c44d0fb819c8857432d5d7 |
| institution | Directory Open Access Journal |
| issn | 2602-3040 |
| language | English |
| last_indexed | 2024-04-10T14:02:27Z |
| publishDate | 2020-12-01 |
| publisher | Cukurova University |
| record_format | Article |
| series | Cukurova Medical Journal |
| spelling | doaj.art-b638ea56f4c44d0fb819c8857432d5d72023-02-15T16:10:11ZengCukurova UniversityCukurova Medical Journal2602-30402020-12-014541653166210.17826/cumj.78154148The effect of Ca-Dobesilate over renal scar formation in an experimental pyelonephritis modelGülfiliz Gönlüşen0Aytül Noyan1Ali Anarat2Behcet Simsek3Aysun Karabay Bayazıt4ÇUKUROVA ÜNİVERSİTESİ, TIP FAKÜLTESİ, CERRAHİ TIP BİLİMLERİ BÖLÜMÜ, PATOLOJİ ANABİLİM DALIBAŞKENT ÜNİVERSİTESİ, TIP FAKÜLTESİ, DAHİLİ TIP BİLİMLERİ BÖLÜMÜ, ÇOCUK SAĞLIĞI VE HASTALIKLARI ANABİLİM DALI, ÇOCUK NEFROLOJİ BİLİM DALIÇUKUROVA ÜNİVERSİTESİ, TIP FAKÜLTESİ, DAHİLİ TIP BİLİMLERİ BÖLÜMÜ, ÇOCUK SAĞLIĞI VE HASTALIKLARI ANABİLİM DALIİSTANBUL HAYDARPAŞA NUMUNE SAĞLIK UYGULAMA VE ARAŞTIRMA MERKEZİçukurova üniversitesi tıp fakültesi çocuk sağlığı ve hastalıkları anabilimdalı çocuk nefrolojisi bilim dalıPurpose: This study was conducted to evaluate the effects of the drug: Ca-Dobesilate (CaD) which has been in common use in venous insufficiency treatment; on renal scarring and expressions of transforming growth factor beta1 (TGFb1), basic fibroblast growth factor (bFGF) and hepatocyte growth factor-beta (HGF-beta) in a rat pyelonephritis model. Materialw and Methods: Eight pyelonephritis groups, each constituting of 7 rats were developed as no treatment - ciprofloxacin – ciprofloxacin and CaD administered groups; following injecting E Coli (ATCC 25922) into kidney. No treatment given rat groups were sacrificed following 24h, 72 h, 14d and 28d from bacterial seeding respectively. Rats from treatment groups were sacrificed after 14d and 28d accordingly. Diagnoses of pyelonephritis and fibrosis, TGFb, bFGF and HGF-beta were scored semiquantitatively by immunohistochemical staining. Results: The extent of pyelonephritis and fibrosis was lower in rats treated with ciprofloxacin and CaD compared to sole ciprofloxacin and no treatment administered counterparts among groups terminated after 2wks following bacterial inoculation. However, CaD effect on pyelonephritis and fibrosis scores did not persist after treatment was discontinued. Conclusion: CaD might alleviate pyelonephritis and scarring, depending on dosage and treatment period and further studies are needed to determine optimum treatment dose and duration.https://dergipark.org.tr/tr/download/article-file/1244164ca-dobesilate,pyelonephritis,pyelonephritis model,kidney fibrosis,renal scar,renal fibrosisca-dobesilatekidney fibrosispyelonephritis modelpyelonephritis, |
| spellingShingle | Gülfiliz Gönlüşen Aytül Noyan Ali Anarat Behcet Simsek Aysun Karabay Bayazıt The effect of Ca-Dobesilate over renal scar formation in an experimental pyelonephritis model Cukurova Medical Journal ca-dobesilate, pyelonephritis, pyelonephritis model, kidney fibrosis, renal scar, renal fibrosis ca-dobesilate kidney fibrosis pyelonephritis model pyelonephritis, |
| title | The effect of Ca-Dobesilate over renal scar formation in an experimental pyelonephritis model |
| title_full | The effect of Ca-Dobesilate over renal scar formation in an experimental pyelonephritis model |
| title_fullStr | The effect of Ca-Dobesilate over renal scar formation in an experimental pyelonephritis model |
| title_full_unstemmed | The effect of Ca-Dobesilate over renal scar formation in an experimental pyelonephritis model |
| title_short | The effect of Ca-Dobesilate over renal scar formation in an experimental pyelonephritis model |
| title_sort | effect of ca dobesilate over renal scar formation in an experimental pyelonephritis model |
| topic | ca-dobesilate, pyelonephritis, pyelonephritis model, kidney fibrosis, renal scar, renal fibrosis ca-dobesilate kidney fibrosis pyelonephritis model pyelonephritis, |
| url | https://dergipark.org.tr/tr/download/article-file/1244164 |
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