The Alpha Subunit of Mitochondrial Processing Peptidase Participated in Fertility Restoration in Honglian-CMS Rice
The cytoplasmic male sterility (CMS) and nuclear-controlled fertility restoration system is a favorable tool for the utilization of heterosis in plant hybrid breeding. Many <i>restorer-of-fertility</i> (<i>Rf</i>) genes have been characterized in various species over the deca...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/6/5442 |
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| author | Weibo Zhao Han Geng Zhiwu Dan Yafei Zeng Mingyue Wang Wuwu Xu Zhongli Hu Wenchao Huang |
| author_facet | Weibo Zhao Han Geng Zhiwu Dan Yafei Zeng Mingyue Wang Wuwu Xu Zhongli Hu Wenchao Huang |
| author_sort | Weibo Zhao |
| collection | DOAJ |
| description | The cytoplasmic male sterility (CMS) and nuclear-controlled fertility restoration system is a favorable tool for the utilization of heterosis in plant hybrid breeding. Many <i>restorer-of-fertility</i> (<i>Rf</i>) genes have been characterized in various species over the decades, but more detailed work is needed to investigate the fertility restoration mechanism. Here, we identified an alpha subunit of mitochondrial processing peptidase (MPPA) that is involved in the fertility restoration process in Honglian-CMS rice. MPPA is a mitochondrial localized protein and interacted with the RF6 protein encoded by the <i>Rf6</i>. MPPA indirectly interacted with hexokinase 6, namely another partner of RF6, to form a protein complex with the same molecular weight as the mitochondrial F<sub>1</sub>F<sub>0</sub>-ATP synthase in processing the CMS transcript. Loss-of-function of <i>MPPA</i> resulted in a defect in pollen fertility, the <i>mppa</i><sup>+/−</sup> heterozygotes showed semi-sterility phenotype and the accumulation of CMS-associated protein ORFH79, showing restrained processing of the CMS-associated <i>atp6-OrfH79</i> in the mutant plant. Taken together, these results threw new light on the process of fertility restoration by investigating the RF6 fertility restoration complex. They also reveal the connections between signal peptide cleavage and the fertility restoration process in Honglian-CMS rice. |
| first_indexed | 2024-03-11T06:26:22Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T06:26:22Z |
| publishDate | 2023-03-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b63bdef812b64a8186b39848142279c92023-11-17T11:34:00ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01246544210.3390/ijms24065442The Alpha Subunit of Mitochondrial Processing Peptidase Participated in Fertility Restoration in Honglian-CMS RiceWeibo Zhao0Han Geng1Zhiwu Dan2Yafei Zeng3Mingyue Wang4Wuwu Xu5Zhongli Hu6Wenchao Huang7State Key Laboratory of Hybrid Rice, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, Wuhan University, Wuhan 430072, ChinaThe cytoplasmic male sterility (CMS) and nuclear-controlled fertility restoration system is a favorable tool for the utilization of heterosis in plant hybrid breeding. Many <i>restorer-of-fertility</i> (<i>Rf</i>) genes have been characterized in various species over the decades, but more detailed work is needed to investigate the fertility restoration mechanism. Here, we identified an alpha subunit of mitochondrial processing peptidase (MPPA) that is involved in the fertility restoration process in Honglian-CMS rice. MPPA is a mitochondrial localized protein and interacted with the RF6 protein encoded by the <i>Rf6</i>. MPPA indirectly interacted with hexokinase 6, namely another partner of RF6, to form a protein complex with the same molecular weight as the mitochondrial F<sub>1</sub>F<sub>0</sub>-ATP synthase in processing the CMS transcript. Loss-of-function of <i>MPPA</i> resulted in a defect in pollen fertility, the <i>mppa</i><sup>+/−</sup> heterozygotes showed semi-sterility phenotype and the accumulation of CMS-associated protein ORFH79, showing restrained processing of the CMS-associated <i>atp6-OrfH79</i> in the mutant plant. Taken together, these results threw new light on the process of fertility restoration by investigating the RF6 fertility restoration complex. They also reveal the connections between signal peptide cleavage and the fertility restoration process in Honglian-CMS rice.https://www.mdpi.com/1422-0067/24/6/5442cytoplasmic male sterilityfertility restorationHonglian-CMS ricemitochondrial processing peptidasesubunit |
| spellingShingle | Weibo Zhao Han Geng Zhiwu Dan Yafei Zeng Mingyue Wang Wuwu Xu Zhongli Hu Wenchao Huang The Alpha Subunit of Mitochondrial Processing Peptidase Participated in Fertility Restoration in Honglian-CMS Rice International Journal of Molecular Sciences cytoplasmic male sterility fertility restoration Honglian-CMS rice mitochondrial processing peptidase subunit |
| title | The Alpha Subunit of Mitochondrial Processing Peptidase Participated in Fertility Restoration in Honglian-CMS Rice |
| title_full | The Alpha Subunit of Mitochondrial Processing Peptidase Participated in Fertility Restoration in Honglian-CMS Rice |
| title_fullStr | The Alpha Subunit of Mitochondrial Processing Peptidase Participated in Fertility Restoration in Honglian-CMS Rice |
| title_full_unstemmed | The Alpha Subunit of Mitochondrial Processing Peptidase Participated in Fertility Restoration in Honglian-CMS Rice |
| title_short | The Alpha Subunit of Mitochondrial Processing Peptidase Participated in Fertility Restoration in Honglian-CMS Rice |
| title_sort | alpha subunit of mitochondrial processing peptidase participated in fertility restoration in honglian cms rice |
| topic | cytoplasmic male sterility fertility restoration Honglian-CMS rice mitochondrial processing peptidase subunit |
| url | https://www.mdpi.com/1422-0067/24/6/5442 |
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