DGAT2 revealed by the immunogold technique in Arabidopsis thaliana lipid bodies associated with microtubules DGAT2 revealed by the immunogold technique in Arabidopsis thaliana lipid bodies associated with microtubules
The immunogold technique with anti-diacylglycerol acyltransferase 2 (DGAT2) antibody revealed in<br />A. thaliana embryo and root meristematic cells gold particles manifesting the presence of DGAT2 in ER as well<br />as in lipid bodies. This being so, lipid synthesis coul...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Via Medica
2012-10-01
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| Series: | Folia Histochemica et Cytobiologica |
| Online Access: | http://czasopisma.viamedica.pl/fhc/article/view/19752 |
| _version_ | 1818138754182807552 |
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| author | Maria Kwiatkowska Dariusz Stępiński Katarzyna Popłońska Agnieszka Wojtczak Justyna T. Polit |
| author_facet | Maria Kwiatkowska Dariusz Stępiński Katarzyna Popłońska Agnieszka Wojtczak Justyna T. Polit |
| author_sort | Maria Kwiatkowska |
| collection | DOAJ |
| description | The immunogold technique with anti-diacylglycerol acyltransferase 2 (DGAT2) antibody revealed in<br />A. thaliana embryo and root meristematic cells gold particles manifesting the presence of DGAT2 in ER as well<br />as in lipid bodies. This being so, lipid synthesis could take place both in ER and in the lipid bodies. The presence<br />of microtubules around the lipid bodies was evidenced under transmission EM. Detection of tubulin around the<br />lipid bodies using the immunogold technique with anti-a-tubulin is in agreement with the above observations.<br />Connection of lipid bodies with microtubules was also detected by us in other plants where they probably participated<br />in lipid synthesis. A similar phenomenon may take place in A. thaliana.<br>The immunogold technique with anti-diacylglycerol acyltransferase 2 (DGAT2) antibody revealed in<br />A. thaliana embryo and root meristematic cells gold particles manifesting the presence of DGAT2 in ER as well<br />as in lipid bodies. This being so, lipid synthesis could take place both in ER and in the lipid bodies. The presence<br />of microtubules around the lipid bodies was evidenced under transmission EM. Detection of tubulin around the<br />lipid bodies using the immunogold technique with anti-a-tubulin is in agreement with the above observations.<br />Connection of lipid bodies with microtubules was also detected by us in other plants where they probably participated<br />in lipid synthesis. A similar phenomenon may take place in A. thaliana. |
| first_indexed | 2024-12-11T10:17:13Z |
| format | Article |
| id | doaj.art-b63bf54f9dfc43daabbe7bb8cfdd6742 |
| institution | Directory Open Access Journal |
| issn | 0239-8508 1897-5631 |
| language | English |
| last_indexed | 2024-12-11T10:17:13Z |
| publishDate | 2012-10-01 |
| publisher | Via Medica |
| record_format | Article |
| series | Folia Histochemica et Cytobiologica |
| spelling | doaj.art-b63bf54f9dfc43daabbe7bb8cfdd67422022-12-22T01:11:35ZengVia MedicaFolia Histochemica et Cytobiologica0239-85081897-56312012-10-0150342743110.5603/19752DGAT2 revealed by the immunogold technique in Arabidopsis thaliana lipid bodies associated with microtubules DGAT2 revealed by the immunogold technique in Arabidopsis thaliana lipid bodies associated with microtubulesMaria KwiatkowskaDariusz StępińskiKatarzyna PopłońskaAgnieszka WojtczakJustyna T. PolitThe immunogold technique with anti-diacylglycerol acyltransferase 2 (DGAT2) antibody revealed in<br />A. thaliana embryo and root meristematic cells gold particles manifesting the presence of DGAT2 in ER as well<br />as in lipid bodies. This being so, lipid synthesis could take place both in ER and in the lipid bodies. The presence<br />of microtubules around the lipid bodies was evidenced under transmission EM. Detection of tubulin around the<br />lipid bodies using the immunogold technique with anti-a-tubulin is in agreement with the above observations.<br />Connection of lipid bodies with microtubules was also detected by us in other plants where they probably participated<br />in lipid synthesis. A similar phenomenon may take place in A. thaliana.<br>The immunogold technique with anti-diacylglycerol acyltransferase 2 (DGAT2) antibody revealed in<br />A. thaliana embryo and root meristematic cells gold particles manifesting the presence of DGAT2 in ER as well<br />as in lipid bodies. This being so, lipid synthesis could take place both in ER and in the lipid bodies. The presence<br />of microtubules around the lipid bodies was evidenced under transmission EM. Detection of tubulin around the<br />lipid bodies using the immunogold technique with anti-a-tubulin is in agreement with the above observations.<br />Connection of lipid bodies with microtubules was also detected by us in other plants where they probably participated<br />in lipid synthesis. A similar phenomenon may take place in A. thaliana.http://czasopisma.viamedica.pl/fhc/article/view/19752 |
| spellingShingle | Maria Kwiatkowska Dariusz Stępiński Katarzyna Popłońska Agnieszka Wojtczak Justyna T. Polit DGAT2 revealed by the immunogold technique in Arabidopsis thaliana lipid bodies associated with microtubules DGAT2 revealed by the immunogold technique in Arabidopsis thaliana lipid bodies associated with microtubules Folia Histochemica et Cytobiologica |
| title | DGAT2 revealed by the immunogold technique in Arabidopsis thaliana lipid bodies associated with microtubules DGAT2 revealed by the immunogold technique in Arabidopsis thaliana lipid bodies associated with microtubules |
| title_full | DGAT2 revealed by the immunogold technique in Arabidopsis thaliana lipid bodies associated with microtubules DGAT2 revealed by the immunogold technique in Arabidopsis thaliana lipid bodies associated with microtubules |
| title_fullStr | DGAT2 revealed by the immunogold technique in Arabidopsis thaliana lipid bodies associated with microtubules DGAT2 revealed by the immunogold technique in Arabidopsis thaliana lipid bodies associated with microtubules |
| title_full_unstemmed | DGAT2 revealed by the immunogold technique in Arabidopsis thaliana lipid bodies associated with microtubules DGAT2 revealed by the immunogold technique in Arabidopsis thaliana lipid bodies associated with microtubules |
| title_short | DGAT2 revealed by the immunogold technique in Arabidopsis thaliana lipid bodies associated with microtubules DGAT2 revealed by the immunogold technique in Arabidopsis thaliana lipid bodies associated with microtubules |
| title_sort | dgat2 revealed by the immunogold technique in arabidopsis thaliana lipid bodies associated with microtubules dgat2 revealed by the immunogold technique in arabidopsis thaliana lipid bodies associated with microtubules |
| url | http://czasopisma.viamedica.pl/fhc/article/view/19752 |
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