Increasing the Sustainability of Maize Grain Production by Using Arbuscular Mycorrhizal Fungi Does Not Affect the Rumen of Dairy Cattle (Bos taurus) and Buffalo (Bubalus bubalis)
New approaches are needed to improve the sustainability of feed production and utilization by ruminants. Promising approaches include increased use of buffaloes for more sustainable milk production, and arbuscular mycorrhizal fungi (AMF) to reduce crop production input needs. However, studies assess...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-10-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fvets.2020.556764/full |
| _version_ | 1818161472679706624 |
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| author | Antonella Chiariotti Joan E. Edwards Gerben D. A. Hermes Gennaro Catillo David Meo Zilio Sabrina Di Giovanni Hauke Smidt Luca Buttazzoni |
| author_facet | Antonella Chiariotti Joan E. Edwards Gerben D. A. Hermes Gennaro Catillo David Meo Zilio Sabrina Di Giovanni Hauke Smidt Luca Buttazzoni |
| author_sort | Antonella Chiariotti |
| collection | DOAJ |
| description | New approaches are needed to improve the sustainability of feed production and utilization by ruminants. Promising approaches include increased use of buffaloes for more sustainable milk production, and arbuscular mycorrhizal fungi (AMF) to reduce crop production input needs. However, studies assessing the effect of crops grown in the presence of AMF on rumen microbial utilization are limited. Based on current knowledge, we hypothesized that maize grain grown on AMF-inoculated soil affected ruminal fermentation and microbiota, and that this effect differed between buffalo and cattle. A dietary cross-over study (four weeks per diet) was conducted using rumen-cannulated cattle (n = 5) and buffalo (n = 6) to assess the effect of maize grain (3.9% (w/v) of diet) grown on soil with or without AMF (15 kg/ha) on ruminal fermentation and microbiota. Production of maize on AMF-treated soil did not affect any of the assessed ruminal fermentation parameters, microbial concentrations, or prokaryotic community composition (using prokaryotic 16S rRNA gene sequence analysis). In contrast, host type had numerous effects. Protozoal counts, lactate, total VFA and isobutyrate, were significantly higher in buffaloes compared to cattle. Conversely, butyrate was significantly lower in buffaloes than in cattle. Host type explained 9.3% of the total variation in prokaryotic community composition, and relative abundance of nine amplicon sequence variants significantly differed between host types. These findings indicate that AMF treatment of maize crops has no detrimental impact on the value of the resulting maize grains as a ruminant feed, and provides additional insight into rumen-based differences between cattle and buffalo. |
| first_indexed | 2024-12-11T16:18:19Z |
| format | Article |
| id | doaj.art-b63c2b57a5174b6a9e7bfd19a9fa27c5 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-11T16:18:19Z |
| publishDate | 2020-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-b63c2b57a5174b6a9e7bfd19a9fa27c52022-12-22T00:58:54ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692020-10-01710.3389/fvets.2020.556764556764Increasing the Sustainability of Maize Grain Production by Using Arbuscular Mycorrhizal Fungi Does Not Affect the Rumen of Dairy Cattle (Bos taurus) and Buffalo (Bubalus bubalis)Antonella Chiariotti0Joan E. Edwards1Gerben D. A. Hermes2Gennaro Catillo3David Meo Zilio4Sabrina Di Giovanni5Hauke Smidt6Luca Buttazzoni7Council for Agricultural Research and Economics (CREA), Research Center for Animal Production and Aquaculture, Monterotondo, ItalyLaboratory of Microbiology, Wageningen University & Research, Wageningen, NetherlandsLaboratory of Microbiology, Wageningen University & Research, Wageningen, NetherlandsCouncil for Agricultural Research and Economics (CREA), Research Center for Animal Production and Aquaculture, Monterotondo, ItalyCouncil for Agricultural Research and Economics (CREA), Research Center for Animal Production and Aquaculture, Monterotondo, ItalyCouncil for Agricultural Research and Economics (CREA), Research Center for Animal Production and Aquaculture, Monterotondo, ItalyLaboratory of Microbiology, Wageningen University & Research, Wageningen, NetherlandsCouncil for Agricultural Research and Economics (CREA), Research Center for Animal Production and Aquaculture, Monterotondo, ItalyNew approaches are needed to improve the sustainability of feed production and utilization by ruminants. Promising approaches include increased use of buffaloes for more sustainable milk production, and arbuscular mycorrhizal fungi (AMF) to reduce crop production input needs. However, studies assessing the effect of crops grown in the presence of AMF on rumen microbial utilization are limited. Based on current knowledge, we hypothesized that maize grain grown on AMF-inoculated soil affected ruminal fermentation and microbiota, and that this effect differed between buffalo and cattle. A dietary cross-over study (four weeks per diet) was conducted using rumen-cannulated cattle (n = 5) and buffalo (n = 6) to assess the effect of maize grain (3.9% (w/v) of diet) grown on soil with or without AMF (15 kg/ha) on ruminal fermentation and microbiota. Production of maize on AMF-treated soil did not affect any of the assessed ruminal fermentation parameters, microbial concentrations, or prokaryotic community composition (using prokaryotic 16S rRNA gene sequence analysis). In contrast, host type had numerous effects. Protozoal counts, lactate, total VFA and isobutyrate, were significantly higher in buffaloes compared to cattle. Conversely, butyrate was significantly lower in buffaloes than in cattle. Host type explained 9.3% of the total variation in prokaryotic community composition, and relative abundance of nine amplicon sequence variants significantly differed between host types. These findings indicate that AMF treatment of maize crops has no detrimental impact on the value of the resulting maize grains as a ruminant feed, and provides additional insight into rumen-based differences between cattle and buffalo.https://www.frontiersin.org/article/10.3389/fvets.2020.556764/fullrumen microbiomebacteriaarchaeaprotozoaanaerobic fungifermentation |
| spellingShingle | Antonella Chiariotti Joan E. Edwards Gerben D. A. Hermes Gennaro Catillo David Meo Zilio Sabrina Di Giovanni Hauke Smidt Luca Buttazzoni Increasing the Sustainability of Maize Grain Production by Using Arbuscular Mycorrhizal Fungi Does Not Affect the Rumen of Dairy Cattle (Bos taurus) and Buffalo (Bubalus bubalis) Frontiers in Veterinary Science rumen microbiome bacteria archaea protozoa anaerobic fungi fermentation |
| title | Increasing the Sustainability of Maize Grain Production by Using Arbuscular Mycorrhizal Fungi Does Not Affect the Rumen of Dairy Cattle (Bos taurus) and Buffalo (Bubalus bubalis) |
| title_full | Increasing the Sustainability of Maize Grain Production by Using Arbuscular Mycorrhizal Fungi Does Not Affect the Rumen of Dairy Cattle (Bos taurus) and Buffalo (Bubalus bubalis) |
| title_fullStr | Increasing the Sustainability of Maize Grain Production by Using Arbuscular Mycorrhizal Fungi Does Not Affect the Rumen of Dairy Cattle (Bos taurus) and Buffalo (Bubalus bubalis) |
| title_full_unstemmed | Increasing the Sustainability of Maize Grain Production by Using Arbuscular Mycorrhizal Fungi Does Not Affect the Rumen of Dairy Cattle (Bos taurus) and Buffalo (Bubalus bubalis) |
| title_short | Increasing the Sustainability of Maize Grain Production by Using Arbuscular Mycorrhizal Fungi Does Not Affect the Rumen of Dairy Cattle (Bos taurus) and Buffalo (Bubalus bubalis) |
| title_sort | increasing the sustainability of maize grain production by using arbuscular mycorrhizal fungi does not affect the rumen of dairy cattle bos taurus and buffalo bubalus bubalis |
| topic | rumen microbiome bacteria archaea protozoa anaerobic fungi fermentation |
| url | https://www.frontiersin.org/article/10.3389/fvets.2020.556764/full |
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