A Dexamethasone-Loaded Polymeric Electrospun Construct as a Tubular Cardiovascular Implant
Cardiovascular tissue engineering is providing many solutions to cardiovascular diseases. The complex disease demands necessitating tissue-engineered constructs with enhanced functionality. In this study, we are presenting the production of a dexamethasone (DEX)-loaded electrospun tubular polymeric...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/15/21/4332 |
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| author | Stavroula Kyriakou Sergio Acosta Ikram El Maachi Stephan Rütten Stefan Jockenhoevel |
| author_facet | Stavroula Kyriakou Sergio Acosta Ikram El Maachi Stephan Rütten Stefan Jockenhoevel |
| author_sort | Stavroula Kyriakou |
| collection | DOAJ |
| description | Cardiovascular tissue engineering is providing many solutions to cardiovascular diseases. The complex disease demands necessitating tissue-engineered constructs with enhanced functionality. In this study, we are presenting the production of a dexamethasone (DEX)-loaded electrospun tubular polymeric poly(<span style="font-variant: small-caps;">l</span>-lactide) (PLA) or poly(<span style="font-variant: small-caps;">d,l</span>-lactide-<i>co</i>-glycolide) (PLGA) construct which contains iPSC-CMs (induced pluripotent stem cell cardiomyocytes), HUVSMCs (human umbilical vein smooth muscle cells), and HUVECs (human umbilical vein endothelial cells) embedded in fibrin gel. The electrospun tube diameter was calculated, as well as the DEX release for 50 days for 2 different DEX concentrations. Furthermore, we investigated the influence of the polymer composition and concentration on the function of the fibrin gels by imaging and quantification of CD31, alpha-smooth muscle actin (αSMA), collagen I (col I), sarcomeric alpha actinin (SAA), and Connexin 43 (Cx43). We evaluated the cytotoxicity and cell proliferation of HUVECs and HUVSMCs cultivated in PLA and PLGA polymeric sheets. The immunohistochemistry results showed efficient iPSC-CM marker expression, while the HUVEC toxicity was higher than the respective HUVSMC value. In total, our study emphasizes the combination of fibrin gel and electrospinning in a functionalized construct, which includes three cell types and provides useful insights of the DEX release and cytotoxicity in a tissue engineering perspective. |
| first_indexed | 2024-03-11T11:22:36Z |
| format | Article |
| id | doaj.art-b6424f5461db437e80b36739c0bad162 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-11T11:22:36Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-b6424f5461db437e80b36739c0bad1622023-11-10T15:10:56ZengMDPI AGPolymers2073-43602023-11-011521433210.3390/polym15214332A Dexamethasone-Loaded Polymeric Electrospun Construct as a Tubular Cardiovascular ImplantStavroula Kyriakou0Sergio Acosta1Ikram El Maachi2Stephan Rütten3Stefan Jockenhoevel4Department of Biohybrid & Medical Textiles (BioTex), AME—Institute of Applied Medical Engineering, Helmholtz Institute, RWTH Aachen University, 52074 Aachen, GermanyDepartment of Biohybrid & Medical Textiles (BioTex), AME—Institute of Applied Medical Engineering, Helmholtz Institute, RWTH Aachen University, 52074 Aachen, GermanyDepartment of Biohybrid & Medical Textiles (BioTex), AME—Institute of Applied Medical Engineering, Helmholtz Institute, RWTH Aachen University, 52074 Aachen, GermanyElectron Microscopy Facility, University Hospital RWTH Aachen, 52074 Aachen, GermanyDepartment of Biohybrid & Medical Textiles (BioTex), AME—Institute of Applied Medical Engineering, Helmholtz Institute, RWTH Aachen University, 52074 Aachen, GermanyCardiovascular tissue engineering is providing many solutions to cardiovascular diseases. The complex disease demands necessitating tissue-engineered constructs with enhanced functionality. In this study, we are presenting the production of a dexamethasone (DEX)-loaded electrospun tubular polymeric poly(<span style="font-variant: small-caps;">l</span>-lactide) (PLA) or poly(<span style="font-variant: small-caps;">d,l</span>-lactide-<i>co</i>-glycolide) (PLGA) construct which contains iPSC-CMs (induced pluripotent stem cell cardiomyocytes), HUVSMCs (human umbilical vein smooth muscle cells), and HUVECs (human umbilical vein endothelial cells) embedded in fibrin gel. The electrospun tube diameter was calculated, as well as the DEX release for 50 days for 2 different DEX concentrations. Furthermore, we investigated the influence of the polymer composition and concentration on the function of the fibrin gels by imaging and quantification of CD31, alpha-smooth muscle actin (αSMA), collagen I (col I), sarcomeric alpha actinin (SAA), and Connexin 43 (Cx43). We evaluated the cytotoxicity and cell proliferation of HUVECs and HUVSMCs cultivated in PLA and PLGA polymeric sheets. The immunohistochemistry results showed efficient iPSC-CM marker expression, while the HUVEC toxicity was higher than the respective HUVSMC value. In total, our study emphasizes the combination of fibrin gel and electrospinning in a functionalized construct, which includes three cell types and provides useful insights of the DEX release and cytotoxicity in a tissue engineering perspective.https://www.mdpi.com/2073-4360/15/21/4332iPSC-CMspoly(<span style="font-variant: small-caps">l</span>-lactide) (PLA)poly(<span style="font-variant: small-caps">d,l</span>-lactide-<i>co</i>-glycolide) (PLGA)fibrin gel |
| spellingShingle | Stavroula Kyriakou Sergio Acosta Ikram El Maachi Stephan Rütten Stefan Jockenhoevel A Dexamethasone-Loaded Polymeric Electrospun Construct as a Tubular Cardiovascular Implant Polymers iPSC-CMs poly(<span style="font-variant: small-caps">l</span>-lactide) (PLA) poly(<span style="font-variant: small-caps">d,l</span>-lactide-<i>co</i>-glycolide) (PLGA) fibrin gel |
| title | A Dexamethasone-Loaded Polymeric Electrospun Construct as a Tubular Cardiovascular Implant |
| title_full | A Dexamethasone-Loaded Polymeric Electrospun Construct as a Tubular Cardiovascular Implant |
| title_fullStr | A Dexamethasone-Loaded Polymeric Electrospun Construct as a Tubular Cardiovascular Implant |
| title_full_unstemmed | A Dexamethasone-Loaded Polymeric Electrospun Construct as a Tubular Cardiovascular Implant |
| title_short | A Dexamethasone-Loaded Polymeric Electrospun Construct as a Tubular Cardiovascular Implant |
| title_sort | dexamethasone loaded polymeric electrospun construct as a tubular cardiovascular implant |
| topic | iPSC-CMs poly(<span style="font-variant: small-caps">l</span>-lactide) (PLA) poly(<span style="font-variant: small-caps">d,l</span>-lactide-<i>co</i>-glycolide) (PLGA) fibrin gel |
| url | https://www.mdpi.com/2073-4360/15/21/4332 |
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