Effect of Probiotic <i>Lactobacillus plantarum</i> on <i>Streptococcus mutans</i> and <i>Candida albicans</i> Clinical Isolates from Children with Early Childhood Caries
Probiotics interfere with pathogenic microorganisms or reinstate the natural microbiome. <i>Streptococcus mutans</i> and <i>Candida albicans</i> are well-known emerging pathogenic bacteria/fungi for dental caries. In this study, three probiotic <i>Lactobacilli</i>...
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/3/2991 |
| _version_ | 1797624162332704768 |
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| author | Yan Zeng Ahmed Fadaak Nora Alomeir Yan Wu Tong Tong Wu Shuang Qing Jin Xiao |
| author_facet | Yan Zeng Ahmed Fadaak Nora Alomeir Yan Wu Tong Tong Wu Shuang Qing Jin Xiao |
| author_sort | Yan Zeng |
| collection | DOAJ |
| description | Probiotics interfere with pathogenic microorganisms or reinstate the natural microbiome. <i>Streptococcus mutans</i> and <i>Candida albicans</i> are well-known emerging pathogenic bacteria/fungi for dental caries. In this study, three probiotic <i>Lactobacilli</i> strains (<i>Lactobacillus plantarum</i> 8014, <i>L. plantarum</i> 14917, and <i>Lactobacillus salivarius</i> 11741) were tested on <i>S. mutans</i> and <i>C. albicans</i> clinical isolates using a multispecies biofilm model simulating clinical cariogenic conditions. The ten pairs of clinical isolates of <i>S. mutans</i> and <i>C. albicans</i> were obtained from children with severe early childhood caries. Our study findings show a remarkable inhibitory effect of <i>L. plantarum</i> 14917 on <i>S. mutans</i> and <i>C. albicans</i> clinical isolates, resulting in significantly reduced growth of <i>S. mutans</i> and <i>C. albicans</i>, a compromised biofilm structure with a significantly smaller microbial and extracellular matrix and a less virulent microcolony structure. FurTre, plantaricin, an antimicrobial peptide produced by <i>L. plantarum</i>, inhibited the growth of <i>S. mutans</i> and <i>C. albicans</i>. The mechanistic assessment indicated that <i>L. plantarum</i> 14917 had a positive inhibitory impact on the expression of <i>S. mutans</i> and <i>C. albicans</i> virulence genes and virulent structure, such as <i>C. albicans</i> hypha formation. Future utilization of <i>L. plantarum</i> 14917 and/or its antimicrobial peptide plantaricin could lead to a new paradigm shift in dental caries prevention. |
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| language | English |
| last_indexed | 2024-03-11T09:39:12Z |
| publishDate | 2023-02-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b6429358500c4da7a9bac7ef421e0e9a2023-11-16T17:05:25ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01243299110.3390/ijms24032991Effect of Probiotic <i>Lactobacillus plantarum</i> on <i>Streptococcus mutans</i> and <i>Candida albicans</i> Clinical Isolates from Children with Early Childhood CariesYan Zeng0Ahmed Fadaak1Nora Alomeir2Yan Wu3Tong Tong Wu4Shuang Qing5Jin Xiao6Eastman Institute for Oral Health, University of Rochester Medical Center, Rochester, NY 14642, USAEastman Institute for Oral Health, University of Rochester Medical Center, Rochester, NY 14642, USAEastman Institute for Oral Health, University of Rochester Medical Center, Rochester, NY 14642, USAEastman Institute for Oral Health, University of Rochester Medical Center, Rochester, NY 14642, USADepartment of Biostatistics and Computational Biology, University of Rochester Medical Center, Rochester, NY 14642, USACollege of Dental Medicine, Columbia University, New York, NY 10032, USAEastman Institute for Oral Health, University of Rochester Medical Center, Rochester, NY 14642, USAProbiotics interfere with pathogenic microorganisms or reinstate the natural microbiome. <i>Streptococcus mutans</i> and <i>Candida albicans</i> are well-known emerging pathogenic bacteria/fungi for dental caries. In this study, three probiotic <i>Lactobacilli</i> strains (<i>Lactobacillus plantarum</i> 8014, <i>L. plantarum</i> 14917, and <i>Lactobacillus salivarius</i> 11741) were tested on <i>S. mutans</i> and <i>C. albicans</i> clinical isolates using a multispecies biofilm model simulating clinical cariogenic conditions. The ten pairs of clinical isolates of <i>S. mutans</i> and <i>C. albicans</i> were obtained from children with severe early childhood caries. Our study findings show a remarkable inhibitory effect of <i>L. plantarum</i> 14917 on <i>S. mutans</i> and <i>C. albicans</i> clinical isolates, resulting in significantly reduced growth of <i>S. mutans</i> and <i>C. albicans</i>, a compromised biofilm structure with a significantly smaller microbial and extracellular matrix and a less virulent microcolony structure. FurTre, plantaricin, an antimicrobial peptide produced by <i>L. plantarum</i>, inhibited the growth of <i>S. mutans</i> and <i>C. albicans</i>. The mechanistic assessment indicated that <i>L. plantarum</i> 14917 had a positive inhibitory impact on the expression of <i>S. mutans</i> and <i>C. albicans</i> virulence genes and virulent structure, such as <i>C. albicans</i> hypha formation. Future utilization of <i>L. plantarum</i> 14917 and/or its antimicrobial peptide plantaricin could lead to a new paradigm shift in dental caries prevention.https://www.mdpi.com/1422-0067/24/3/2991<i>Streptococcus mutans</i><i>Candida albicans</i><i>Lactobacillus plantarum</i>probioticsmultispecies biofilm |
| spellingShingle | Yan Zeng Ahmed Fadaak Nora Alomeir Yan Wu Tong Tong Wu Shuang Qing Jin Xiao Effect of Probiotic <i>Lactobacillus plantarum</i> on <i>Streptococcus mutans</i> and <i>Candida albicans</i> Clinical Isolates from Children with Early Childhood Caries International Journal of Molecular Sciences <i>Streptococcus mutans</i> <i>Candida albicans</i> <i>Lactobacillus plantarum</i> probiotics multispecies biofilm |
| title | Effect of Probiotic <i>Lactobacillus plantarum</i> on <i>Streptococcus mutans</i> and <i>Candida albicans</i> Clinical Isolates from Children with Early Childhood Caries |
| title_full | Effect of Probiotic <i>Lactobacillus plantarum</i> on <i>Streptococcus mutans</i> and <i>Candida albicans</i> Clinical Isolates from Children with Early Childhood Caries |
| title_fullStr | Effect of Probiotic <i>Lactobacillus plantarum</i> on <i>Streptococcus mutans</i> and <i>Candida albicans</i> Clinical Isolates from Children with Early Childhood Caries |
| title_full_unstemmed | Effect of Probiotic <i>Lactobacillus plantarum</i> on <i>Streptococcus mutans</i> and <i>Candida albicans</i> Clinical Isolates from Children with Early Childhood Caries |
| title_short | Effect of Probiotic <i>Lactobacillus plantarum</i> on <i>Streptococcus mutans</i> and <i>Candida albicans</i> Clinical Isolates from Children with Early Childhood Caries |
| title_sort | effect of probiotic i lactobacillus plantarum i on i streptococcus mutans i and i candida albicans i clinical isolates from children with early childhood caries |
| topic | <i>Streptococcus mutans</i> <i>Candida albicans</i> <i>Lactobacillus plantarum</i> probiotics multispecies biofilm |
| url | https://www.mdpi.com/1422-0067/24/3/2991 |
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