Protocol for assessing ex vivo lipolysis of murine adipose tissue
Summary: Here, we provide a detailed protocol for assessing ex vivo lipolysis of subcutaneous and visceral white adipose tissue. We describe a robust approach to detect depot-specific changes in lipolytic potential under basal and beta-adrenergic receptor-stimulated conditions. Given that adipose ti...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-09-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722003987 |
| _version_ | 1811330861603225600 |
|---|---|
| author | Debasish Roy Julia M. Myers Andrea Tedeschi |
| author_facet | Debasish Roy Julia M. Myers Andrea Tedeschi |
| author_sort | Debasish Roy |
| collection | DOAJ |
| description | Summary: Here, we provide a detailed protocol for assessing ex vivo lipolysis of subcutaneous and visceral white adipose tissue. We describe a robust approach to detect depot-specific changes in lipolytic potential under basal and beta-adrenergic receptor-stimulated conditions. Given that adipose tissue plays a critical role in systemic metabolic health, this experimental protocol can be used to determine changes in adipose tissue function in health and disease. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-13T16:09:15Z |
| format | Article |
| id | doaj.art-b648c2a1ba194d5a946ef822039bd297 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-13T16:09:15Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-b648c2a1ba194d5a946ef822039bd2972022-12-22T02:40:18ZengElsevierSTAR Protocols2666-16672022-09-0133101518Protocol for assessing ex vivo lipolysis of murine adipose tissueDebasish Roy0Julia M. Myers1Andrea Tedeschi2Department of Neuroscience, Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA; Corresponding authorDepartment of Neuroscience, Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USADepartment of Neuroscience, Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA; Discovery Theme on Chronic Brain Injury, The Ohio State University, Columbus, OH 43210, USA; Corresponding authorSummary: Here, we provide a detailed protocol for assessing ex vivo lipolysis of subcutaneous and visceral white adipose tissue. We describe a robust approach to detect depot-specific changes in lipolytic potential under basal and beta-adrenergic receptor-stimulated conditions. Given that adipose tissue plays a critical role in systemic metabolic health, this experimental protocol can be used to determine changes in adipose tissue function in health and disease. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722003987Cell-based AssaysMetabolism |
| spellingShingle | Debasish Roy Julia M. Myers Andrea Tedeschi Protocol for assessing ex vivo lipolysis of murine adipose tissue STAR Protocols Cell-based Assays Metabolism |
| title | Protocol for assessing ex vivo lipolysis of murine adipose tissue |
| title_full | Protocol for assessing ex vivo lipolysis of murine adipose tissue |
| title_fullStr | Protocol for assessing ex vivo lipolysis of murine adipose tissue |
| title_full_unstemmed | Protocol for assessing ex vivo lipolysis of murine adipose tissue |
| title_short | Protocol for assessing ex vivo lipolysis of murine adipose tissue |
| title_sort | protocol for assessing ex vivo lipolysis of murine adipose tissue |
| topic | Cell-based Assays Metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722003987 |
| work_keys_str_mv | AT debasishroy protocolforassessingexvivolipolysisofmurineadiposetissue AT juliammyers protocolforassessingexvivolipolysisofmurineadiposetissue AT andreatedeschi protocolforassessingexvivolipolysisofmurineadiposetissue |