Protocol for assessing ex vivo lipolysis of murine adipose tissue
Summary: Here, we provide a detailed protocol for assessing ex vivo lipolysis of subcutaneous and visceral white adipose tissue. We describe a robust approach to detect depot-specific changes in lipolytic potential under basal and beta-adrenergic receptor-stimulated conditions. Given that adipose ti...
Main Authors: | , , |
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Format: | Article |
Language: | English |
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Elsevier
2022-09-01
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Series: | STAR Protocols |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722003987 |
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author | Debasish Roy Julia M. Myers Andrea Tedeschi |
author_facet | Debasish Roy Julia M. Myers Andrea Tedeschi |
author_sort | Debasish Roy |
collection | DOAJ |
description | Summary: Here, we provide a detailed protocol for assessing ex vivo lipolysis of subcutaneous and visceral white adipose tissue. We describe a robust approach to detect depot-specific changes in lipolytic potential under basal and beta-adrenergic receptor-stimulated conditions. Given that adipose tissue plays a critical role in systemic metabolic health, this experimental protocol can be used to determine changes in adipose tissue function in health and disease. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
first_indexed | 2024-04-13T16:09:15Z |
format | Article |
id | doaj.art-b648c2a1ba194d5a946ef822039bd297 |
institution | Directory Open Access Journal |
issn | 2666-1667 |
language | English |
last_indexed | 2024-04-13T16:09:15Z |
publishDate | 2022-09-01 |
publisher | Elsevier |
record_format | Article |
series | STAR Protocols |
spelling | doaj.art-b648c2a1ba194d5a946ef822039bd2972022-12-22T02:40:18ZengElsevierSTAR Protocols2666-16672022-09-0133101518Protocol for assessing ex vivo lipolysis of murine adipose tissueDebasish Roy0Julia M. Myers1Andrea Tedeschi2Department of Neuroscience, Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA; Corresponding authorDepartment of Neuroscience, Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USADepartment of Neuroscience, Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA; Discovery Theme on Chronic Brain Injury, The Ohio State University, Columbus, OH 43210, USA; Corresponding authorSummary: Here, we provide a detailed protocol for assessing ex vivo lipolysis of subcutaneous and visceral white adipose tissue. We describe a robust approach to detect depot-specific changes in lipolytic potential under basal and beta-adrenergic receptor-stimulated conditions. Given that adipose tissue plays a critical role in systemic metabolic health, this experimental protocol can be used to determine changes in adipose tissue function in health and disease. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722003987Cell-based AssaysMetabolism |
spellingShingle | Debasish Roy Julia M. Myers Andrea Tedeschi Protocol for assessing ex vivo lipolysis of murine adipose tissue STAR Protocols Cell-based Assays Metabolism |
title | Protocol for assessing ex vivo lipolysis of murine adipose tissue |
title_full | Protocol for assessing ex vivo lipolysis of murine adipose tissue |
title_fullStr | Protocol for assessing ex vivo lipolysis of murine adipose tissue |
title_full_unstemmed | Protocol for assessing ex vivo lipolysis of murine adipose tissue |
title_short | Protocol for assessing ex vivo lipolysis of murine adipose tissue |
title_sort | protocol for assessing ex vivo lipolysis of murine adipose tissue |
topic | Cell-based Assays Metabolism |
url | http://www.sciencedirect.com/science/article/pii/S2666166722003987 |
work_keys_str_mv | AT debasishroy protocolforassessingexvivolipolysisofmurineadiposetissue AT juliammyers protocolforassessingexvivolipolysisofmurineadiposetissue AT andreatedeschi protocolforassessingexvivolipolysisofmurineadiposetissue |