A Rabbit Model for Peripheral Nerve Reconstruction Studies Avoiding Automutilation Behavior
Background The rabbit sciatic nerve injury model may represent a valuable alternative for critical gap distance seen in humans but often leads to automutilation. In this study, we modified the complete sciatic nerve injury model for avoiding autophagy. Materials and Methods In 20 adult fe...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Georg Thieme Verlag KG
2022-01-01
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| Series: | Journal of Brachial Plexus and Peripheral Nerve Injury |
| Subjects: | |
| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.1055/s-0042-1747959 |
| _version_ | 1811242857866985472 |
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| author | Jonathan A. Sorkin Ziv Rechany Mara Almog Nina Dietzmeyer Yuval Shapira Kirsten Haastert-Talini Shimon Rochkind |
| author_facet | Jonathan A. Sorkin Ziv Rechany Mara Almog Nina Dietzmeyer Yuval Shapira Kirsten Haastert-Talini Shimon Rochkind |
| author_sort | Jonathan A. Sorkin |
| collection | DOAJ |
| description | Background The rabbit sciatic nerve injury model may represent a valuable alternative for critical gap distance seen in humans but often leads to automutilation. In this study, we modified the complete sciatic nerve injury model for avoiding autophagy.
Materials and Methods In 20 adult female New Zealand White rabbits, instead of transecting the complete sciatic nerve, we unilaterally transected the tibial portion and preserved the peroneal portion. Thereby loss of sensation in the dorsal aspect of the paw was avoided. The tibial portion was repaired in a reversed autograft approach in a length of 2.6 cm. In an alternative repair approach, a gap of 2.6 cm in length was repaired with a chitosan-based nerve guide.
Results During the 6-month follow-up period, there were no incidents of autotomy. Nerve regeneration of the tibial portion of the sciatic nerve was evaluated histologically and morphometrically. A clear difference between the distal segments of the healthy contralateral and the repaired tibial portion of the sciatic nerve was detectable, validating the model.
Conclusion By transecting the isolated tibial portion of the rabbit sciatic nerve and leaving the peroneal portion intact, it was possible to eliminate automutilation behavior. |
| first_indexed | 2024-04-12T13:58:10Z |
| format | Article |
| id | doaj.art-b64c29330ae843eabb3753f3500f2ca8 |
| institution | Directory Open Access Journal |
| issn | 1749-7221 |
| language | English |
| last_indexed | 2024-04-12T13:58:10Z |
| publishDate | 2022-01-01 |
| publisher | Georg Thieme Verlag KG |
| record_format | Article |
| series | Journal of Brachial Plexus and Peripheral Nerve Injury |
| spelling | doaj.art-b64c29330ae843eabb3753f3500f2ca82022-12-22T03:30:19ZengGeorg Thieme Verlag KGJournal of Brachial Plexus and Peripheral Nerve Injury1749-72212022-01-011701e22e2910.1055/s-0042-1747959A Rabbit Model for Peripheral Nerve Reconstruction Studies Avoiding Automutilation BehaviorJonathan A. Sorkin0Ziv Rechany1Mara Almog2Nina Dietzmeyer3Yuval Shapira4Kirsten Haastert-Talini5Shimon Rochkind6Research Center for Nerve Reconstruction, Department of Neurosurgery, Tel Aviv Sourasky Medical Center, Tel Aviv, IsraelResearch Center for Nerve Reconstruction, Department of Neurosurgery, Tel Aviv Sourasky Medical Center, Tel Aviv, IsraelResearch Center for Nerve Reconstruction, Department of Neurosurgery, Tel Aviv Sourasky Medical Center, Tel Aviv, IsraelInstitute of Neuroanatomy and Cell Biology, Hannover Medical School, Hannover, GermanyDivision of Peripheral Nerve Reconstruction, Department of Neurosurgery, Tel Aviv Sourasky Medical Center, Tel Aviv University, Tel Aviv, IsraelInstitute of Neuroanatomy and Cell Biology, Hannover Medical School, Hannover, GermanyResearch Center for Nerve Reconstruction, Department of Neurosurgery, Tel Aviv Sourasky Medical Center, Tel Aviv, IsraelBackground The rabbit sciatic nerve injury model may represent a valuable alternative for critical gap distance seen in humans but often leads to automutilation. In this study, we modified the complete sciatic nerve injury model for avoiding autophagy. Materials and Methods In 20 adult female New Zealand White rabbits, instead of transecting the complete sciatic nerve, we unilaterally transected the tibial portion and preserved the peroneal portion. Thereby loss of sensation in the dorsal aspect of the paw was avoided. The tibial portion was repaired in a reversed autograft approach in a length of 2.6 cm. In an alternative repair approach, a gap of 2.6 cm in length was repaired with a chitosan-based nerve guide. Results During the 6-month follow-up period, there were no incidents of autotomy. Nerve regeneration of the tibial portion of the sciatic nerve was evaluated histologically and morphometrically. A clear difference between the distal segments of the healthy contralateral and the repaired tibial portion of the sciatic nerve was detectable, validating the model. Conclusion By transecting the isolated tibial portion of the rabbit sciatic nerve and leaving the peroneal portion intact, it was possible to eliminate automutilation behavior.http://www.thieme-connect.de/DOI/DOI?10.1055/s-0042-1747959peripheral nerve reconstructionrabbit modelautomutilation behavior |
| spellingShingle | Jonathan A. Sorkin Ziv Rechany Mara Almog Nina Dietzmeyer Yuval Shapira Kirsten Haastert-Talini Shimon Rochkind A Rabbit Model for Peripheral Nerve Reconstruction Studies Avoiding Automutilation Behavior Journal of Brachial Plexus and Peripheral Nerve Injury peripheral nerve reconstruction rabbit model automutilation behavior |
| title | A Rabbit Model for Peripheral Nerve Reconstruction Studies Avoiding Automutilation Behavior |
| title_full | A Rabbit Model for Peripheral Nerve Reconstruction Studies Avoiding Automutilation Behavior |
| title_fullStr | A Rabbit Model for Peripheral Nerve Reconstruction Studies Avoiding Automutilation Behavior |
| title_full_unstemmed | A Rabbit Model for Peripheral Nerve Reconstruction Studies Avoiding Automutilation Behavior |
| title_short | A Rabbit Model for Peripheral Nerve Reconstruction Studies Avoiding Automutilation Behavior |
| title_sort | rabbit model for peripheral nerve reconstruction studies avoiding automutilation behavior |
| topic | peripheral nerve reconstruction rabbit model automutilation behavior |
| url | http://www.thieme-connect.de/DOI/DOI?10.1055/s-0042-1747959 |
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