The Neuroprotective Effects of Spray-Dried Porcine Plasma Supplementation Involve the Microbiota−Gut−Brain Axis
Dietary supplementation with spray-dried porcine plasma (SDP) reduces the Alzheimer’s disease (AD) hallmarks in SAMP8 mice. Since gut microbiota can play a critical role in the AD progression, we have studied if the neuroprotective effects of SDP involve the microbiota−gut−brain axis. Experiments we...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
|
| Series: | Nutrients |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6643/14/11/2211 |
| _version_ | 1797492248636555264 |
|---|---|
| author | Cristina Rosell-Cardona Concepció Amat Christian Griñán-Ferré Javier Polo Mercè Pallàs Anna Pérez-Bosque Miquel Moretó Lluïsa Miró |
| author_facet | Cristina Rosell-Cardona Concepció Amat Christian Griñán-Ferré Javier Polo Mercè Pallàs Anna Pérez-Bosque Miquel Moretó Lluïsa Miró |
| author_sort | Cristina Rosell-Cardona |
| collection | DOAJ |
| description | Dietary supplementation with spray-dried porcine plasma (SDP) reduces the Alzheimer’s disease (AD) hallmarks in SAMP8 mice. Since gut microbiota can play a critical role in the AD progression, we have studied if the neuroprotective effects of SDP involve the microbiota−gut−brain axis. Experiments were performed on two-month-old SAMP8 mice fed a standard diet and on six-month-old SAMP8 mice fed a control diet or an 8% SDP supplemented diet for four months. Senescence impaired short- and long-term memory, reduced cortical brain-derived neurotrophic factor (BDNF) abundance, increased interleukin (<i>Il)-1</i><i>β</i>, <i>Il-6</i>, and Toll-like receptor 2 <i>(Tlr2)</i> expression, and reduced transforming growth factor <i>β</i> (<i>Tgf-</i><i>β)</i> expression and IL-10 concentration (all <i>p</i> < 0.05) and these effects were mitigated by SDP (all <i>p</i> < 0.05). Aging also increased pro-inflammatory cytokines in serum and colon (all <i>p</i> < 0.05). SDP attenuated both colonic and systemic inflammation in aged mice (all <i>p</i> < 0.05). SDP induced the proliferation of health-promoting bacteria, such as <i>Lactobacillus</i> and <i>Pediococcus</i>, while reducing the abundance of inflammation-associated bacteria, such as <i>Johnsonella</i> and <i>Erysipelothrix</i> (both <i>q</i> < 0.1). In conclusion, SDP has mucosal and systemic anti-inflammatory effects as well as neuroprotective properties in senescent mice; these effects are well correlated with SDP promotion of the abundance of probiotic species, which indicates that the gut–brain axis could be involved in the peripheral effects of SDP supplementation. |
| first_indexed | 2024-03-10T01:00:54Z |
| format | Article |
| id | doaj.art-b64d6674c52248018a3cc85f6856603e |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T01:00:54Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-b64d6674c52248018a3cc85f6856603e2023-11-23T14:35:23ZengMDPI AGNutrients2072-66432022-05-011411221110.3390/nu14112211The Neuroprotective Effects of Spray-Dried Porcine Plasma Supplementation Involve the Microbiota−Gut−Brain AxisCristina Rosell-Cardona0Concepció Amat1Christian Griñán-Ferré2Javier Polo3Mercè Pallàs4Anna Pérez-Bosque5Miquel Moretó6Lluïsa Miró7Department of Biochemistry and Physiology, Faculty of Pharmacy and Food Sciences, Institute for Nutrition and Food Safety, Universitat de Barcelona (UB), 08028 Barcelona, SpainDepartment of Biochemistry and Physiology, Faculty of Pharmacy and Food Sciences, Institute for Nutrition and Food Safety, Universitat de Barcelona (UB), 08028 Barcelona, SpainDepartment of Pharmacology, Toxicology, and Medicinal Chemistry, Faculty of Pharmacy and Food Sciences, Institute of Neurosciences, Universitat de Barcelona (UB), 08028 Barcelona, SpainAPC-Europe S.L.U., 08403 Granollers, SpainDepartment of Pharmacology, Toxicology, and Medicinal Chemistry, Faculty of Pharmacy and Food Sciences, Institute of Neurosciences, Universitat de Barcelona (UB), 08028 Barcelona, SpainDepartment of Biochemistry and Physiology, Faculty of Pharmacy and Food Sciences, Institute for Nutrition and Food Safety, Universitat de Barcelona (UB), 08028 Barcelona, SpainDepartment of Biochemistry and Physiology, Faculty of Pharmacy and Food Sciences, Institute for Nutrition and Food Safety, Universitat de Barcelona (UB), 08028 Barcelona, SpainDepartment of Biochemistry and Physiology, Faculty of Pharmacy and Food Sciences, Institute for Nutrition and Food Safety, Universitat de Barcelona (UB), 08028 Barcelona, SpainDietary supplementation with spray-dried porcine plasma (SDP) reduces the Alzheimer’s disease (AD) hallmarks in SAMP8 mice. Since gut microbiota can play a critical role in the AD progression, we have studied if the neuroprotective effects of SDP involve the microbiota−gut−brain axis. Experiments were performed on two-month-old SAMP8 mice fed a standard diet and on six-month-old SAMP8 mice fed a control diet or an 8% SDP supplemented diet for four months. Senescence impaired short- and long-term memory, reduced cortical brain-derived neurotrophic factor (BDNF) abundance, increased interleukin (<i>Il)-1</i><i>β</i>, <i>Il-6</i>, and Toll-like receptor 2 <i>(Tlr2)</i> expression, and reduced transforming growth factor <i>β</i> (<i>Tgf-</i><i>β)</i> expression and IL-10 concentration (all <i>p</i> < 0.05) and these effects were mitigated by SDP (all <i>p</i> < 0.05). Aging also increased pro-inflammatory cytokines in serum and colon (all <i>p</i> < 0.05). SDP attenuated both colonic and systemic inflammation in aged mice (all <i>p</i> < 0.05). SDP induced the proliferation of health-promoting bacteria, such as <i>Lactobacillus</i> and <i>Pediococcus</i>, while reducing the abundance of inflammation-associated bacteria, such as <i>Johnsonella</i> and <i>Erysipelothrix</i> (both <i>q</i> < 0.1). In conclusion, SDP has mucosal and systemic anti-inflammatory effects as well as neuroprotective properties in senescent mice; these effects are well correlated with SDP promotion of the abundance of probiotic species, which indicates that the gut–brain axis could be involved in the peripheral effects of SDP supplementation.https://www.mdpi.com/2072-6643/14/11/2211microbiotaAlzheimer’s diseaseagingdietary supplementationspray-dried porcine plasma |
| spellingShingle | Cristina Rosell-Cardona Concepció Amat Christian Griñán-Ferré Javier Polo Mercè Pallàs Anna Pérez-Bosque Miquel Moretó Lluïsa Miró The Neuroprotective Effects of Spray-Dried Porcine Plasma Supplementation Involve the Microbiota−Gut−Brain Axis Nutrients microbiota Alzheimer’s disease aging dietary supplementation spray-dried porcine plasma |
| title | The Neuroprotective Effects of Spray-Dried Porcine Plasma Supplementation Involve the Microbiota−Gut−Brain Axis |
| title_full | The Neuroprotective Effects of Spray-Dried Porcine Plasma Supplementation Involve the Microbiota−Gut−Brain Axis |
| title_fullStr | The Neuroprotective Effects of Spray-Dried Porcine Plasma Supplementation Involve the Microbiota−Gut−Brain Axis |
| title_full_unstemmed | The Neuroprotective Effects of Spray-Dried Porcine Plasma Supplementation Involve the Microbiota−Gut−Brain Axis |
| title_short | The Neuroprotective Effects of Spray-Dried Porcine Plasma Supplementation Involve the Microbiota−Gut−Brain Axis |
| title_sort | neuroprotective effects of spray dried porcine plasma supplementation involve the microbiota gut brain axis |
| topic | microbiota Alzheimer’s disease aging dietary supplementation spray-dried porcine plasma |
| url | https://www.mdpi.com/2072-6643/14/11/2211 |
| work_keys_str_mv | AT cristinarosellcardona theneuroprotectiveeffectsofspraydriedporcineplasmasupplementationinvolvethemicrobiotagutbrainaxis AT concepcioamat theneuroprotectiveeffectsofspraydriedporcineplasmasupplementationinvolvethemicrobiotagutbrainaxis AT christiangrinanferre theneuroprotectiveeffectsofspraydriedporcineplasmasupplementationinvolvethemicrobiotagutbrainaxis AT javierpolo theneuroprotectiveeffectsofspraydriedporcineplasmasupplementationinvolvethemicrobiotagutbrainaxis AT mercepallas theneuroprotectiveeffectsofspraydriedporcineplasmasupplementationinvolvethemicrobiotagutbrainaxis AT annaperezbosque theneuroprotectiveeffectsofspraydriedporcineplasmasupplementationinvolvethemicrobiotagutbrainaxis AT miquelmoreto theneuroprotectiveeffectsofspraydriedporcineplasmasupplementationinvolvethemicrobiotagutbrainaxis AT lluisamiro theneuroprotectiveeffectsofspraydriedporcineplasmasupplementationinvolvethemicrobiotagutbrainaxis AT cristinarosellcardona neuroprotectiveeffectsofspraydriedporcineplasmasupplementationinvolvethemicrobiotagutbrainaxis AT concepcioamat neuroprotectiveeffectsofspraydriedporcineplasmasupplementationinvolvethemicrobiotagutbrainaxis AT christiangrinanferre neuroprotectiveeffectsofspraydriedporcineplasmasupplementationinvolvethemicrobiotagutbrainaxis AT javierpolo neuroprotectiveeffectsofspraydriedporcineplasmasupplementationinvolvethemicrobiotagutbrainaxis AT mercepallas neuroprotectiveeffectsofspraydriedporcineplasmasupplementationinvolvethemicrobiotagutbrainaxis AT annaperezbosque neuroprotectiveeffectsofspraydriedporcineplasmasupplementationinvolvethemicrobiotagutbrainaxis AT miquelmoreto neuroprotectiveeffectsofspraydriedporcineplasmasupplementationinvolvethemicrobiotagutbrainaxis AT lluisamiro neuroprotectiveeffectsofspraydriedporcineplasmasupplementationinvolvethemicrobiotagutbrainaxis |