Long non-coding RNA KCNQ1 opposite strand/antisense transcript 1, a potential biomarker for glaucoma, accelerates glaucoma progression via microRNA-93-5p/Homeobox box 3 axis
Background: Glaucoma is marked by retinal neuron death in the ganglion cell layer, leading to irreversible vision loss. Aberrant long non-coding RNA (lncRNA) expression is associated with glaucoma. The study was to explore the latent molecular mechanism of lncRNA KCNQ1 opposite strand/antisense tran...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Electronic Journal of Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345823000349 |
| _version_ | 1797359992310857728 |
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| author | ZhaoLian Xie Hui Wang LinLin Liu HaiQing Zhang Jing Liu |
| author_facet | ZhaoLian Xie Hui Wang LinLin Liu HaiQing Zhang Jing Liu |
| author_sort | ZhaoLian Xie |
| collection | DOAJ |
| description | Background: Glaucoma is marked by retinal neuron death in the ganglion cell layer, leading to irreversible vision loss. Aberrant long non-coding RNA (lncRNA) expression is associated with glaucoma. The study was to explore the latent molecular mechanism of lncRNA KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1) in N-methyl-D-aspartate (NMDA)-stimulated glaucoma. Results: The data demonstrated that KCNQ1OT1 expression was elevated in glaucoma patients, serving as a diagnostic biomarker of glaucoma. Rats injected with NMDA developed visual loss and retinopathy and expressed high KCNQ1OT1. After treating retinal ganglion cells (RGCs) with NMDA, cell proliferation was suppressed and apoptosis was augmented. Silenced KCNQ1OT1 or HOXB3 or elevated miR-93-5p alleviated NMDA-induced suppression of RGC growth. KCNQ1OT1 mediated miR-93-5p expression by targeting homeobox box 3 (HOXB3). The protection of silenced KCNQ1OT1 in NMDA-treated RGCs was turned around by elevated HOXB3. Conclusions: Overall, KCNQ1OT1 accelerates glaucoma progression via miR-93-5p/HOXB3 axis.How to cite: Xie ZL, Wang H, Liu LL, et al. Long non-coding RNA KCNQ1 opposite strand/antisense transcript 1, a potential biomarker for glaucoma, accelerates glaucoma progression via microRNA-93-5p/Homeobox box 3 axis. Electron J Biotechnol 2024;67 . https://doi.org/10.1016/j.ejbt.2023.10.002. |
| first_indexed | 2024-03-08T15:31:54Z |
| format | Article |
| id | doaj.art-b65198edd95e4d1596c5ce3cbe9be306 |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-03-08T15:31:54Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-b65198edd95e4d1596c5ce3cbe9be3062024-01-10T04:34:28ZengElsevierElectronic Journal of Biotechnology0717-34582024-01-01672333Long non-coding RNA KCNQ1 opposite strand/antisense transcript 1, a potential biomarker for glaucoma, accelerates glaucoma progression via microRNA-93-5p/Homeobox box 3 axisZhaoLian Xie0Hui Wang1LinLin Liu2HaiQing Zhang3Jing Liu4Corresponding author.; Department of Ophthalmology, The First Affiliated Hospital of Gannan Medical College, Ganzhou City, Jiangxi Province 341000, ChinaDepartment of Ophthalmology, The First Affiliated Hospital of Gannan Medical College, Ganzhou City, Jiangxi Province 341000, ChinaDepartment of Ophthalmology, The First Affiliated Hospital of Gannan Medical College, Ganzhou City, Jiangxi Province 341000, ChinaDepartment of Ophthalmology, The First Affiliated Hospital of Gannan Medical College, Ganzhou City, Jiangxi Province 341000, ChinaDepartment of Ophthalmology, The First Affiliated Hospital of Gannan Medical College, Ganzhou City, Jiangxi Province 341000, ChinaBackground: Glaucoma is marked by retinal neuron death in the ganglion cell layer, leading to irreversible vision loss. Aberrant long non-coding RNA (lncRNA) expression is associated with glaucoma. The study was to explore the latent molecular mechanism of lncRNA KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1) in N-methyl-D-aspartate (NMDA)-stimulated glaucoma. Results: The data demonstrated that KCNQ1OT1 expression was elevated in glaucoma patients, serving as a diagnostic biomarker of glaucoma. Rats injected with NMDA developed visual loss and retinopathy and expressed high KCNQ1OT1. After treating retinal ganglion cells (RGCs) with NMDA, cell proliferation was suppressed and apoptosis was augmented. Silenced KCNQ1OT1 or HOXB3 or elevated miR-93-5p alleviated NMDA-induced suppression of RGC growth. KCNQ1OT1 mediated miR-93-5p expression by targeting homeobox box 3 (HOXB3). The protection of silenced KCNQ1OT1 in NMDA-treated RGCs was turned around by elevated HOXB3. Conclusions: Overall, KCNQ1OT1 accelerates glaucoma progression via miR-93-5p/HOXB3 axis.How to cite: Xie ZL, Wang H, Liu LL, et al. Long non-coding RNA KCNQ1 opposite strand/antisense transcript 1, a potential biomarker for glaucoma, accelerates glaucoma progression via microRNA-93-5p/Homeobox box 3 axis. Electron J Biotechnol 2024;67 . https://doi.org/10.1016/j.ejbt.2023.10.002.http://www.sciencedirect.com/science/article/pii/S0717345823000349Antisense transcriptBiomarkersGlaucomaHomeobox box 3Long non-coding RNAMicroRNA-93-5p |
| spellingShingle | ZhaoLian Xie Hui Wang LinLin Liu HaiQing Zhang Jing Liu Long non-coding RNA KCNQ1 opposite strand/antisense transcript 1, a potential biomarker for glaucoma, accelerates glaucoma progression via microRNA-93-5p/Homeobox box 3 axis Electronic Journal of Biotechnology Antisense transcript Biomarkers Glaucoma Homeobox box 3 Long non-coding RNA MicroRNA-93-5p |
| title | Long non-coding RNA KCNQ1 opposite strand/antisense transcript 1, a potential biomarker for glaucoma, accelerates glaucoma progression via microRNA-93-5p/Homeobox box 3 axis |
| title_full | Long non-coding RNA KCNQ1 opposite strand/antisense transcript 1, a potential biomarker for glaucoma, accelerates glaucoma progression via microRNA-93-5p/Homeobox box 3 axis |
| title_fullStr | Long non-coding RNA KCNQ1 opposite strand/antisense transcript 1, a potential biomarker for glaucoma, accelerates glaucoma progression via microRNA-93-5p/Homeobox box 3 axis |
| title_full_unstemmed | Long non-coding RNA KCNQ1 opposite strand/antisense transcript 1, a potential biomarker for glaucoma, accelerates glaucoma progression via microRNA-93-5p/Homeobox box 3 axis |
| title_short | Long non-coding RNA KCNQ1 opposite strand/antisense transcript 1, a potential biomarker for glaucoma, accelerates glaucoma progression via microRNA-93-5p/Homeobox box 3 axis |
| title_sort | long non coding rna kcnq1 opposite strand antisense transcript 1 a potential biomarker for glaucoma accelerates glaucoma progression via microrna 93 5p homeobox box 3 axis |
| topic | Antisense transcript Biomarkers Glaucoma Homeobox box 3 Long non-coding RNA MicroRNA-93-5p |
| url | http://www.sciencedirect.com/science/article/pii/S0717345823000349 |
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