Expression profile of miRNA-29a in neural cells
<p><strong>Objective </strong>To investigate the expression profile of microRNA (miRNA)-29a in mice neural cells. <strong>Methods</strong> Quantitative real-time polymerase chain reaction (qRT-PCR) was used to analyze miRNA-29a expression changes of neurons and astr...
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| Format: | Article |
| Language: | English |
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Tianjin Huanhu Hospital
2015-01-01
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| Series: | Chinese Journal of Contemporary Neurology and Neurosurgery |
| Subjects: | |
| Online Access: | http://www.cjcnn.org/index.php/cjcnn/article/view/1119 |
| _version_ | 1819031109616271360 |
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| author | Yu LU Min-yu JIAN Ye LI Ru-quan HAN |
| author_facet | Yu LU Min-yu JIAN Ye LI Ru-quan HAN |
| author_sort | Yu LU |
| collection | DOAJ |
| description | <p><strong>Objective </strong>To investigate the expression profile of microRNA (miRNA)-29a in mice neural cells. <strong>Methods</strong> Quantitative real-time polymerase chain reaction (qRT-PCR) was used to analyze miRNA-29a expression changes of neurons and astrocytes after 3, 7, 15, 21 d of primary culture in vitro, and cortex tissue of 3, 7, 15, 21 d old mice. <strong>Results</strong> miRNA-29a expression increased in nervous system with the growth of mice. Compared with the 3th day, miRNA-29a relative expression on the 7th, 15th, 21th days in neurons, astrocytes and cortex tissue were obviously higher (<em>P</em> < 0.05 or <em>P</em> < 0.01). At each time point, the relative expression levels of miRNA-29a in cultured astrocytes were 20-40 times higher than in cultured neurons (<em>P</em> = 0.000), and levels in cortex tissue were about 1/3 that seen in cultured astrocytes (<em>P</em> = 0.000). <strong>Conclusions </strong> With the growth and development of brain cortex of mice, miRNA-29a is highly expressed in astrocytes, reflecting its abundant and specific expression.</p><p> </p><p><strong>DOI: </strong>10.3969/j.issn.1672-6731.2015.01.009</p> |
| first_indexed | 2024-12-21T06:40:49Z |
| format | Article |
| id | doaj.art-b652206b89dd4fc79468db3a123223c2 |
| institution | Directory Open Access Journal |
| issn | 1672-6731 |
| language | English |
| last_indexed | 2024-12-21T06:40:49Z |
| publishDate | 2015-01-01 |
| publisher | Tianjin Huanhu Hospital |
| record_format | Article |
| series | Chinese Journal of Contemporary Neurology and Neurosurgery |
| spelling | doaj.art-b652206b89dd4fc79468db3a123223c22022-12-21T19:12:43ZengTianjin Huanhu HospitalChinese Journal of Contemporary Neurology and Neurosurgery1672-67312015-01-0115139421113Expression profile of miRNA-29a in neural cellsYu LU0Min-yu JIAN1Ye LI2Ru-quan HAN3Department of Anesthesia, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, ChinaDepartment of Anesthesia, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, ChinaDepartment of Anesthesia, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, ChinaDepartment of Anesthesia, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China<p><strong>Objective </strong>To investigate the expression profile of microRNA (miRNA)-29a in mice neural cells. <strong>Methods</strong> Quantitative real-time polymerase chain reaction (qRT-PCR) was used to analyze miRNA-29a expression changes of neurons and astrocytes after 3, 7, 15, 21 d of primary culture in vitro, and cortex tissue of 3, 7, 15, 21 d old mice. <strong>Results</strong> miRNA-29a expression increased in nervous system with the growth of mice. Compared with the 3th day, miRNA-29a relative expression on the 7th, 15th, 21th days in neurons, astrocytes and cortex tissue were obviously higher (<em>P</em> < 0.05 or <em>P</em> < 0.01). At each time point, the relative expression levels of miRNA-29a in cultured astrocytes were 20-40 times higher than in cultured neurons (<em>P</em> = 0.000), and levels in cortex tissue were about 1/3 that seen in cultured astrocytes (<em>P</em> = 0.000). <strong>Conclusions </strong> With the growth and development of brain cortex of mice, miRNA-29a is highly expressed in astrocytes, reflecting its abundant and specific expression.</p><p> </p><p><strong>DOI: </strong>10.3969/j.issn.1672-6731.2015.01.009</p>http://www.cjcnn.org/index.php/cjcnn/article/view/1119MicroRNAsAstrocytesNeuronsCerebral cortexPolymerase chain reactionCells, cultured |
| spellingShingle | Yu LU Min-yu JIAN Ye LI Ru-quan HAN Expression profile of miRNA-29a in neural cells Chinese Journal of Contemporary Neurology and Neurosurgery MicroRNAs Astrocytes Neurons Cerebral cortex Polymerase chain reaction Cells, cultured |
| title | Expression profile of miRNA-29a in neural cells |
| title_full | Expression profile of miRNA-29a in neural cells |
| title_fullStr | Expression profile of miRNA-29a in neural cells |
| title_full_unstemmed | Expression profile of miRNA-29a in neural cells |
| title_short | Expression profile of miRNA-29a in neural cells |
| title_sort | expression profile of mirna 29a in neural cells |
| topic | MicroRNAs Astrocytes Neurons Cerebral cortex Polymerase chain reaction Cells, cultured |
| url | http://www.cjcnn.org/index.php/cjcnn/article/view/1119 |
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