Inflammatory cytokine detection in adenotonsill and peripheral blood mononuclear cells- culture in adenotonsillectomy patients: a comparative study
Background: Tonsils and adenoid hypertrophy is a major respiratory symptom in children which is partly due to recruitment of inflammatory cells in upper airway lymph nodes as a result of the effects of synthesis and release of different inflammatory cytokines. It seems that infections play role in c...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | fas |
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Tehran University of Medical Sciences
2013-04-01
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| Series: | Tehran University Medical Journal |
| Subjects: | |
| Online Access: | http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-35&slc_lang=en&sid=1 |
| _version_ | 1818524397134151680 |
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| author | Farhadi M Tabatabaei A Shekarabi M Noorbakhsh S Shokrollahi MR Javadi Nia Sh Faramarzi M |
| author_facet | Farhadi M Tabatabaei A Shekarabi M Noorbakhsh S Shokrollahi MR Javadi Nia Sh Faramarzi M |
| author_sort | Farhadi M |
| collection | DOAJ |
| description | Background: Tonsils and adenoid hypertrophy is a major respiratory symptom in children which is partly due to recruitment of inflammatory cells in upper airway lymph nodes as a result of the effects of synthesis and release of different inflammatory cytokines. It seems that infections play role in concert with these cytokines leading to tonsilar hypertrophy and other pathologic consequences. It is proposed that cellular infiltrate of tonsils and adenoids may secrete different quantities of these cytokines compared with peripheral blood mononuclear cells (PBMC) cultures.Methods: Among patients who were admitted for adenotonsillectomy to the ENT ward, 37 patients, under 1-12 years old patients with fulfill criteria selected to include the study. Excised adenoid and tonsils cultured and inflammatory cytokines Interferon-γ (INF-γ), Interlukine-1 (IL-1), IL-6, IL-8 and tumor necrosis factor-α (TNF-α) measured in cellular culture supernatant. The same cytokines measured in PBMC cultures.Results: The data shows that there is a significant difference between IFN-γ and IL-8 amounts in adenoid tissue culture supernatant and PBMC culture of our patients. Furth-ermore, the amounts of IFN-γ, IL-1 and IL-8 showed considerable difference between tonsilar tissue culture supernatant and PBMC culture of these patients. Although there is a significant correlation between IL-6 amounts in tissue culture supernatant and PBMC culture (P=0.02), the respective data for TNF is only almost significant.Conclusion: Inflammatory cytokines may have significant role in the early provoke of inflammation occurred in hypertrophied tonsils and adenoid. The majority of these cyt-okines increase the expression of adhesion molecules on epithelial cells and influence the recruitment of leucocytes and inflamed tonsils. On the other hand lack of sufficient cytokine release may lead to persistent infections and may cause chronic inflammation and hypertrophied tissue. |
| first_indexed | 2024-12-11T05:56:35Z |
| format | Article |
| id | doaj.art-b65433d30c4e4825bc5e4d38f053c0b7 |
| institution | Directory Open Access Journal |
| issn | 1683-1764 1735-7322 |
| language | fas |
| last_indexed | 2024-12-11T05:56:35Z |
| publishDate | 2013-04-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Tehran University Medical Journal |
| spelling | doaj.art-b65433d30c4e4825bc5e4d38f053c0b72022-12-22T01:18:39ZfasTehran University of Medical SciencesTehran University Medical Journal1683-17641735-73222013-04-017113136Inflammatory cytokine detection in adenotonsill and peripheral blood mononuclear cells- culture in adenotonsillectomy patients: a comparative studyFarhadi MTabatabaei AShekarabi MNoorbakhsh SShokrollahi MRJavadi Nia ShFaramarzi MBackground: Tonsils and adenoid hypertrophy is a major respiratory symptom in children which is partly due to recruitment of inflammatory cells in upper airway lymph nodes as a result of the effects of synthesis and release of different inflammatory cytokines. It seems that infections play role in concert with these cytokines leading to tonsilar hypertrophy and other pathologic consequences. It is proposed that cellular infiltrate of tonsils and adenoids may secrete different quantities of these cytokines compared with peripheral blood mononuclear cells (PBMC) cultures.Methods: Among patients who were admitted for adenotonsillectomy to the ENT ward, 37 patients, under 1-12 years old patients with fulfill criteria selected to include the study. Excised adenoid and tonsils cultured and inflammatory cytokines Interferon-γ (INF-γ), Interlukine-1 (IL-1), IL-6, IL-8 and tumor necrosis factor-α (TNF-α) measured in cellular culture supernatant. The same cytokines measured in PBMC cultures.Results: The data shows that there is a significant difference between IFN-γ and IL-8 amounts in adenoid tissue culture supernatant and PBMC culture of our patients. Furth-ermore, the amounts of IFN-γ, IL-1 and IL-8 showed considerable difference between tonsilar tissue culture supernatant and PBMC culture of these patients. Although there is a significant correlation between IL-6 amounts in tissue culture supernatant and PBMC culture (P=0.02), the respective data for TNF is only almost significant.Conclusion: Inflammatory cytokines may have significant role in the early provoke of inflammation occurred in hypertrophied tonsils and adenoid. The majority of these cyt-okines increase the expression of adhesion molecules on epithelial cells and influence the recruitment of leucocytes and inflamed tonsils. On the other hand lack of sufficient cytokine release may lead to persistent infections and may cause chronic inflammation and hypertrophied tissue.http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-35&slc_lang=en&sid=1Adenoidschemokinespalatine tonsilperipheral blood mononuclear cells (PBMC) |
| spellingShingle | Farhadi M Tabatabaei A Shekarabi M Noorbakhsh S Shokrollahi MR Javadi Nia Sh Faramarzi M Inflammatory cytokine detection in adenotonsill and peripheral blood mononuclear cells- culture in adenotonsillectomy patients: a comparative study Tehran University Medical Journal Adenoids chemokines palatine tonsil peripheral blood mononuclear cells (PBMC) |
| title | Inflammatory cytokine detection in adenotonsill and peripheral blood mononuclear cells- culture in adenotonsillectomy patients: a comparative study |
| title_full | Inflammatory cytokine detection in adenotonsill and peripheral blood mononuclear cells- culture in adenotonsillectomy patients: a comparative study |
| title_fullStr | Inflammatory cytokine detection in adenotonsill and peripheral blood mononuclear cells- culture in adenotonsillectomy patients: a comparative study |
| title_full_unstemmed | Inflammatory cytokine detection in adenotonsill and peripheral blood mononuclear cells- culture in adenotonsillectomy patients: a comparative study |
| title_short | Inflammatory cytokine detection in adenotonsill and peripheral blood mononuclear cells- culture in adenotonsillectomy patients: a comparative study |
| title_sort | inflammatory cytokine detection in adenotonsill and peripheral blood mononuclear cells culture in adenotonsillectomy patients a comparative study |
| topic | Adenoids chemokines palatine tonsil peripheral blood mononuclear cells (PBMC) |
| url | http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-35&slc_lang=en&sid=1 |
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