In Vitro Cellular Uptake Studies of Self-Assembled Fluorinated Nanoparticles Labelled with Antibodies
Nanoparticles (NPs) functionalized with antibodies (Abs) on their surface are used in a wide range of bioapplications. Whereas the attachment of antibodies to single NPs to trigger the internalization in cells via receptor-mediated endocytosis has been widely studied, the conjugation of antibodies t...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/8/1906 |
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| author | Mona Atabakhshi-Kashi Mónica Carril Hossein Mahdavi Wolfgang J. Parak Carolina Carrillo-Carrion Khosro Khajeh |
| author_facet | Mona Atabakhshi-Kashi Mónica Carril Hossein Mahdavi Wolfgang J. Parak Carolina Carrillo-Carrion Khosro Khajeh |
| author_sort | Mona Atabakhshi-Kashi |
| collection | DOAJ |
| description | Nanoparticles (NPs) functionalized with antibodies (Abs) on their surface are used in a wide range of bioapplications. Whereas the attachment of antibodies to single NPs to trigger the internalization in cells via receptor-mediated endocytosis has been widely studied, the conjugation of antibodies to larger NP assemblies has been much less explored. Taking into account that NP assemblies may be advantageous for some specific applications, the possibility of incorporating targeting ligands is quite important. Herein, we performed the effective conjugation of antibodies onto a fluorescent NP assembly, which consisted of fluorinated Quantum Dots (QD) self-assembled through fluorine–fluorine hydrophobic interactions. Cellular uptake studies by confocal microscopy and flow cytometry revealed that the NP assembly underwent the same uptake procedure as individual NPs; that is, the antibodies retained their targeting ability once attached to the nanoassembly, and the NP assembly preserved its intrinsic properties (i.e., fluorescence in the case of QD nanoassembly). |
| first_indexed | 2024-03-10T08:32:32Z |
| format | Article |
| id | doaj.art-b659270b78c24ef8aaee804cffa4aa12 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T08:32:32Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-b659270b78c24ef8aaee804cffa4aa122023-11-22T08:57:31ZengMDPI AGNanomaterials2079-49912021-07-01118190610.3390/nano11081906In Vitro Cellular Uptake Studies of Self-Assembled Fluorinated Nanoparticles Labelled with AntibodiesMona Atabakhshi-Kashi0Mónica Carril1Hossein Mahdavi2Wolfgang J. Parak3Carolina Carrillo-Carrion4Khosro Khajeh5Department of Nanobiotechnology, Tarbiat Modares University, Tehran 14115-175, IranBioengineered Particles Group, CIC biomaGUNE, 20014 San Sebastian, SpainSchool of Chemistry, College of Science, University of Tehran, Tehran 14174-66191, IranBioengineered Particles Group, CIC biomaGUNE, 20014 San Sebastian, SpainBioengineered Particles Group, CIC biomaGUNE, 20014 San Sebastian, SpainDepartment of Nanobiotechnology, Tarbiat Modares University, Tehran 14115-175, IranNanoparticles (NPs) functionalized with antibodies (Abs) on their surface are used in a wide range of bioapplications. Whereas the attachment of antibodies to single NPs to trigger the internalization in cells via receptor-mediated endocytosis has been widely studied, the conjugation of antibodies to larger NP assemblies has been much less explored. Taking into account that NP assemblies may be advantageous for some specific applications, the possibility of incorporating targeting ligands is quite important. Herein, we performed the effective conjugation of antibodies onto a fluorescent NP assembly, which consisted of fluorinated Quantum Dots (QD) self-assembled through fluorine–fluorine hydrophobic interactions. Cellular uptake studies by confocal microscopy and flow cytometry revealed that the NP assembly underwent the same uptake procedure as individual NPs; that is, the antibodies retained their targeting ability once attached to the nanoassembly, and the NP assembly preserved its intrinsic properties (i.e., fluorescence in the case of QD nanoassembly).https://www.mdpi.com/2079-4991/11/8/1906nanoparticlesquantum dotsantibody conjugationfluorinationself-assembly |
| spellingShingle | Mona Atabakhshi-Kashi Mónica Carril Hossein Mahdavi Wolfgang J. Parak Carolina Carrillo-Carrion Khosro Khajeh In Vitro Cellular Uptake Studies of Self-Assembled Fluorinated Nanoparticles Labelled with Antibodies Nanomaterials nanoparticles quantum dots antibody conjugation fluorination self-assembly |
| title | In Vitro Cellular Uptake Studies of Self-Assembled Fluorinated Nanoparticles Labelled with Antibodies |
| title_full | In Vitro Cellular Uptake Studies of Self-Assembled Fluorinated Nanoparticles Labelled with Antibodies |
| title_fullStr | In Vitro Cellular Uptake Studies of Self-Assembled Fluorinated Nanoparticles Labelled with Antibodies |
| title_full_unstemmed | In Vitro Cellular Uptake Studies of Self-Assembled Fluorinated Nanoparticles Labelled with Antibodies |
| title_short | In Vitro Cellular Uptake Studies of Self-Assembled Fluorinated Nanoparticles Labelled with Antibodies |
| title_sort | in vitro cellular uptake studies of self assembled fluorinated nanoparticles labelled with antibodies |
| topic | nanoparticles quantum dots antibody conjugation fluorination self-assembly |
| url | https://www.mdpi.com/2079-4991/11/8/1906 |
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