Platelet-Derived Microparticle Count in β-Thalassemia Patients with Direct Labeling Monoclonal Antibody CD62P and CD41
Thromboembolic events are potentially life-threatening clinical complications found in β-thalassemia patients. The pathogenesis of the hypercoagulable state in β-thalassemia patients results from the degradation of excess α-globin chains in red blood cells, leading to intracellular labile iron accum...
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| Format: | Article |
| Language: | English |
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Universitas Padjajaran
2020-06-01
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| Series: | Majalah Kedokteran Bandung |
| Subjects: | |
| Online Access: | http://journal.fk.unpad.ac.id/index.php/mkb/article/view/1836 |
| _version_ | 1818148250555777024 |
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| author | Ivan Lumban Toruan Pandji Irani Fianza Delita Prihatni |
| author_facet | Ivan Lumban Toruan Pandji Irani Fianza Delita Prihatni |
| author_sort | Ivan Lumban Toruan |
| collection | DOAJ |
| description | Thromboembolic events are potentially life-threatening clinical complications found in β-thalassemia patients. The pathogenesis of the hypercoagulable state in β-thalassemia patients results from the degradation of excess α-globin chains in red blood cells, leading to intracellular labile iron accumulation, oxidative stress, and more rigid, deformed, and eventually prematurely damaged red blood cells. This process is associated with the loss of the normal asymmetrical distribution of membrane phosphatidylserine and its exposure to the outer surface of the blood cell membrane resulting in the formation of tenase complexes, prothrombinase, and thrombin complexes. Increased thrombins lead to platelet activation and platelet-derived microparticles synthesis, which in turn contributes to thrombus formation. This study aimed to determine the increase in the platelet-derived microparticle count by direct labeling of CD62P and CD41 monoclonal antibodies in β-thalassemia patients when compared with normal subjects. This was a cross-sectional analytical quantitative study conducted in Dr. Hasan Sadikin General Hospital Bandung and Dharmais Cancer Hospital Jakarta Indonesia between August and September 2019. Sixty patients, divided evenly into β-thalassemia group and control group, were labeled by CD62P and CD41 monoclonal antibodies. Results showed that the β-thalassemia group had a platelet count of 197x103/uL (58–1,261) with a median count for platelet-derived microparticles of 10,553 events/uL (779–90,971) as opposed to 1,861 events/uL (1,244–3,174) in the normal group (p<0.05). Therefore, the platelet-derived microparticle count in the β-thalassemia patients is 5.7 times greater than in the normal subjects. |
| first_indexed | 2024-12-11T12:48:09Z |
| format | Article |
| id | doaj.art-b65ce81ce56c4d288664a20ef1f69a8c |
| institution | Directory Open Access Journal |
| issn | 0126-074X 2338-6223 |
| language | English |
| last_indexed | 2024-12-11T12:48:09Z |
| publishDate | 2020-06-01 |
| publisher | Universitas Padjajaran |
| record_format | Article |
| series | Majalah Kedokteran Bandung |
| spelling | doaj.art-b65ce81ce56c4d288664a20ef1f69a8c2022-12-22T01:06:45ZengUniversitas PadjajaranMajalah Kedokteran Bandung0126-074X2338-62232020-06-01522929810.15395/mkb.v52n2.18361199Platelet-Derived Microparticle Count in β-Thalassemia Patients with Direct Labeling Monoclonal Antibody CD62P and CD41Ivan Lumban Toruan0Pandji Irani Fianza1Delita Prihatni2Department of Internal Medicine, Faculty of Medicine, Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital BandungDepartment of Internal Medicine, Faculty of Medicine Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital BandungDepartment of Clinical Pathology, Faculty of Medicine Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital BandungThromboembolic events are potentially life-threatening clinical complications found in β-thalassemia patients. The pathogenesis of the hypercoagulable state in β-thalassemia patients results from the degradation of excess α-globin chains in red blood cells, leading to intracellular labile iron accumulation, oxidative stress, and more rigid, deformed, and eventually prematurely damaged red blood cells. This process is associated with the loss of the normal asymmetrical distribution of membrane phosphatidylserine and its exposure to the outer surface of the blood cell membrane resulting in the formation of tenase complexes, prothrombinase, and thrombin complexes. Increased thrombins lead to platelet activation and platelet-derived microparticles synthesis, which in turn contributes to thrombus formation. This study aimed to determine the increase in the platelet-derived microparticle count by direct labeling of CD62P and CD41 monoclonal antibodies in β-thalassemia patients when compared with normal subjects. This was a cross-sectional analytical quantitative study conducted in Dr. Hasan Sadikin General Hospital Bandung and Dharmais Cancer Hospital Jakarta Indonesia between August and September 2019. Sixty patients, divided evenly into β-thalassemia group and control group, were labeled by CD62P and CD41 monoclonal antibodies. Results showed that the β-thalassemia group had a platelet count of 197x103/uL (58–1,261) with a median count for platelet-derived microparticles of 10,553 events/uL (779–90,971) as opposed to 1,861 events/uL (1,244–3,174) in the normal group (p<0.05). Therefore, the platelet-derived microparticle count in the β-thalassemia patients is 5.7 times greater than in the normal subjects.http://journal.fk.unpad.ac.id/index.php/mkb/article/view/1836hypercoagulation, monoclonal antibodies, phosphatidylserine, platelet derived microparticles, thalassemia. |
| spellingShingle | Ivan Lumban Toruan Pandji Irani Fianza Delita Prihatni Platelet-Derived Microparticle Count in β-Thalassemia Patients with Direct Labeling Monoclonal Antibody CD62P and CD41 Majalah Kedokteran Bandung hypercoagulation, monoclonal antibodies, phosphatidylserine, platelet derived microparticles, thalassemia. |
| title | Platelet-Derived Microparticle Count in β-Thalassemia Patients with Direct Labeling Monoclonal Antibody CD62P and CD41 |
| title_full | Platelet-Derived Microparticle Count in β-Thalassemia Patients with Direct Labeling Monoclonal Antibody CD62P and CD41 |
| title_fullStr | Platelet-Derived Microparticle Count in β-Thalassemia Patients with Direct Labeling Monoclonal Antibody CD62P and CD41 |
| title_full_unstemmed | Platelet-Derived Microparticle Count in β-Thalassemia Patients with Direct Labeling Monoclonal Antibody CD62P and CD41 |
| title_short | Platelet-Derived Microparticle Count in β-Thalassemia Patients with Direct Labeling Monoclonal Antibody CD62P and CD41 |
| title_sort | platelet derived microparticle count in β thalassemia patients with direct labeling monoclonal antibody cd62p and cd41 |
| topic | hypercoagulation, monoclonal antibodies, phosphatidylserine, platelet derived microparticles, thalassemia. |
| url | http://journal.fk.unpad.ac.id/index.php/mkb/article/view/1836 |
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