The fusion of tissue spheroids attached to pre-stretched electrospun polyurethane scaffolds
Effective cell invasion into thick electrospun biomimetic scaffolds is an unsolved problem. One possible strategy to biofabricate tissue constructs of desirable thickness and material properties without the need for cell invasion is to use thin (<2 µm) porous electrospun meshes and self-assemblin...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2014-11-01
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| Series: | Journal of Tissue Engineering |
| Online Access: | https://doi.org/10.1177/2041731414556561 |
| _version_ | 1819011028478853120 |
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| author | Vince Beachley Vladimir Kasyanov Agnes Nagy-Mehesz Russell Norris Iveta Ozolanta Martins Kalejs Peteris Stradins Leandra Baptista Karina da Silva Jose Grainjero Xuejun Wen Vladimir Mironov |
| author_facet | Vince Beachley Vladimir Kasyanov Agnes Nagy-Mehesz Russell Norris Iveta Ozolanta Martins Kalejs Peteris Stradins Leandra Baptista Karina da Silva Jose Grainjero Xuejun Wen Vladimir Mironov |
| author_sort | Vince Beachley |
| collection | DOAJ |
| description | Effective cell invasion into thick electrospun biomimetic scaffolds is an unsolved problem. One possible strategy to biofabricate tissue constructs of desirable thickness and material properties without the need for cell invasion is to use thin (<2 µm) porous electrospun meshes and self-assembling (capable of tissue fusion) tissue spheroids as building blocks. Pre-stretched electrospun meshes remained taut in cell culture and were able to support tissue spheroids with minimal deformation. We hypothesize that elastic electrospun scaffolds could be used as temporal support templates for rapid self-assembly of cell spheroids into higher order tissue structures, such as engineered vascular tissue. The aim of this study was to investigate how the attachment of tissue spheroids to pre-stretched polyurethane scaffolds may interfere with the tissue fusion process. Tissue spheroids attached, spread, and fused after being placed on pre-stretched polyurethane electrospun matrices and formed tissue constructs. Efforts to eliminate hole defects with fibrogenic tissue growth factor-β resulted in the increased synthesis of collagen and periostin and a dramatic reduction in hole size and number. In control experiments, tissue spheroids fuse on a non-adhesive hydrogel and form continuous tissue constructs without holes. Our data demonstrate that tissue spheroids attached to thin stretched elastic electrospun scaffolds have an interrupted tissue fusion process. The resulting tissue-engineered construct phenotype is a direct outcome of the delicate balance of the competing physical forces operating during the tissue fusion process at the interface of the pre-stretched elastic scaffold and the attached tissue spheroids. We have shown that with appropriate treatments, this process can be modulated, and thus, a thin pre-stretched elastic polyurethane electrospun scaffold could serve as a supporting template for rapid biofabrication of thick tissue-engineered constructs without the need for cell invasion. |
| first_indexed | 2024-12-21T01:21:39Z |
| format | Article |
| id | doaj.art-b65fd9c80e064c07a9ef87081e0fa17b |
| institution | Directory Open Access Journal |
| issn | 2041-7314 |
| language | English |
| last_indexed | 2024-12-21T01:21:39Z |
| publishDate | 2014-11-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Tissue Engineering |
| spelling | doaj.art-b65fd9c80e064c07a9ef87081e0fa17b2022-12-21T19:20:38ZengSAGE PublishingJournal of Tissue Engineering2041-73142014-11-01510.1177/204173141455656110.1177_2041731414556561The fusion of tissue spheroids attached to pre-stretched electrospun polyurethane scaffoldsVince Beachley0Vladimir Kasyanov1Agnes Nagy-Mehesz2Russell Norris3Iveta Ozolanta4Martins Kalejs5Peteris Stradins6Leandra Baptista7Karina da Silva8Jose Grainjero9Xuejun Wen10Vladimir Mironov11Department of Biomedical Engineering, Rowan University, Glassboro, NJ, USALaboratory of Biomechanics, Riga Stradins University, Riga, LatviaDepartment of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, USADepartment of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, USALaboratory of Biomechanics, Riga Stradins University, Riga, LatviaDepartment of Cardiac Surgery, Pauls Stradins Clinical University Hospital, Riga, LatviaDepartment of Cardiac Surgery, Pauls Stradins Clinical University Hospital, Riga, LatviaLaboratory of Tissue Engineering, Inmetro, Xerém, Rio de Janeiro, BrazilLaboratory of Tissue Engineering, Inmetro, Xerém, Rio de Janeiro, BrazilLaboratory of Tissue Engineering, Inmetro, Xerém, Rio de Janeiro, BrazilDepartment of Chemical and Life Science Engineering, Virginia Commonwealth University, Richmond, VA, USADivision of 3D Technologies, Renato Archer Center for Information Technology, Campinas, São Paulo, BrazilEffective cell invasion into thick electrospun biomimetic scaffolds is an unsolved problem. One possible strategy to biofabricate tissue constructs of desirable thickness and material properties without the need for cell invasion is to use thin (<2 µm) porous electrospun meshes and self-assembling (capable of tissue fusion) tissue spheroids as building blocks. Pre-stretched electrospun meshes remained taut in cell culture and were able to support tissue spheroids with minimal deformation. We hypothesize that elastic electrospun scaffolds could be used as temporal support templates for rapid self-assembly of cell spheroids into higher order tissue structures, such as engineered vascular tissue. The aim of this study was to investigate how the attachment of tissue spheroids to pre-stretched polyurethane scaffolds may interfere with the tissue fusion process. Tissue spheroids attached, spread, and fused after being placed on pre-stretched polyurethane electrospun matrices and formed tissue constructs. Efforts to eliminate hole defects with fibrogenic tissue growth factor-β resulted in the increased synthesis of collagen and periostin and a dramatic reduction in hole size and number. In control experiments, tissue spheroids fuse on a non-adhesive hydrogel and form continuous tissue constructs without holes. Our data demonstrate that tissue spheroids attached to thin stretched elastic electrospun scaffolds have an interrupted tissue fusion process. The resulting tissue-engineered construct phenotype is a direct outcome of the delicate balance of the competing physical forces operating during the tissue fusion process at the interface of the pre-stretched elastic scaffold and the attached tissue spheroids. We have shown that with appropriate treatments, this process can be modulated, and thus, a thin pre-stretched elastic polyurethane electrospun scaffold could serve as a supporting template for rapid biofabrication of thick tissue-engineered constructs without the need for cell invasion.https://doi.org/10.1177/2041731414556561 |
| spellingShingle | Vince Beachley Vladimir Kasyanov Agnes Nagy-Mehesz Russell Norris Iveta Ozolanta Martins Kalejs Peteris Stradins Leandra Baptista Karina da Silva Jose Grainjero Xuejun Wen Vladimir Mironov The fusion of tissue spheroids attached to pre-stretched electrospun polyurethane scaffolds Journal of Tissue Engineering |
| title | The fusion of tissue spheroids attached to pre-stretched electrospun polyurethane scaffolds |
| title_full | The fusion of tissue spheroids attached to pre-stretched electrospun polyurethane scaffolds |
| title_fullStr | The fusion of tissue spheroids attached to pre-stretched electrospun polyurethane scaffolds |
| title_full_unstemmed | The fusion of tissue spheroids attached to pre-stretched electrospun polyurethane scaffolds |
| title_short | The fusion of tissue spheroids attached to pre-stretched electrospun polyurethane scaffolds |
| title_sort | fusion of tissue spheroids attached to pre stretched electrospun polyurethane scaffolds |
| url | https://doi.org/10.1177/2041731414556561 |
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