Nitrate and nitrite reductase activities of Mycobacterium avium
Background: In spite of the fact that the standard test for nitrate reductase activity is negative for Mycobacterium avium, it can grow in a defined minimal medium with either nitrate (NO3) or nitrite (NO2) as sole nitrogen sources. Methods: NO3-and NO2-reductase activities were measured in soluble...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2018-01-01
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| Series: | International Journal of Mycobacteriology |
| Subjects: | |
| Online Access: | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2018;volume=7;issue=4;spage=328;epage=331;aulast=Butala |
| _version_ | 1818914641217060864 |
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| author | Nitin S Butala Joseph Oliver Falkinham |
| author_facet | Nitin S Butala Joseph Oliver Falkinham |
| author_sort | Nitin S Butala |
| collection | DOAJ |
| description | Background: In spite of the fact that the standard test for nitrate reductase activity is negative for Mycobacterium avium, it can grow in a defined minimal medium with either nitrate (NO3) or nitrite (NO2) as sole nitrogen sources. Methods: NO3-and NO2-reductase activities were measured in soluble and membrane fractions of aerobically grown cells of M. avium and those grown aerobically and shifted to anaerobiosis. Results: NO3- and NO2-reductase activities were only detected in the membrane fractions and the two enzyme activities were significantly reduced if cells were grown aerobically in the presence of ammonia (NH4). The NO2-reductase activity of membrane fractions was 2-fold higher than that of NO3-reductase consistent with the fact that NO3-reductase activity of M. avium cannot be detected if measured by nitrite formation. Membrane fractions of M. avium cells grown 1 week aerobically and then 2 weeks under anaerobic conditions had NO3-and NO2-reductase activities. Conclusion: The results are consistent with the presence of assimilatory NO3-and NO2-reductase activities in cells of M. avium grown under aerobic conditions. Further, the data suggest that a shift to anaerobic conditions results in the appearance of ammonium-insensitive NO3-and NO2-reductase activities; quite possibly that function in a dissimilatory role (redox balancing). |
| first_indexed | 2024-12-19T23:49:37Z |
| format | Article |
| id | doaj.art-b6620970261b41bc8a2ba66a66791628 |
| institution | Directory Open Access Journal |
| issn | 2212-5531 2212-554X |
| language | English |
| last_indexed | 2024-12-19T23:49:37Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | International Journal of Mycobacteriology |
| spelling | doaj.art-b6620970261b41bc8a2ba66a667916282022-12-21T20:01:12ZengWolters Kluwer Medknow PublicationsInternational Journal of Mycobacteriology2212-55312212-554X2018-01-017432833110.4103/ijmy.ijmy_118_18Nitrate and nitrite reductase activities of Mycobacterium aviumNitin S ButalaJoseph Oliver FalkinhamBackground: In spite of the fact that the standard test for nitrate reductase activity is negative for Mycobacterium avium, it can grow in a defined minimal medium with either nitrate (NO3) or nitrite (NO2) as sole nitrogen sources. Methods: NO3-and NO2-reductase activities were measured in soluble and membrane fractions of aerobically grown cells of M. avium and those grown aerobically and shifted to anaerobiosis. Results: NO3- and NO2-reductase activities were only detected in the membrane fractions and the two enzyme activities were significantly reduced if cells were grown aerobically in the presence of ammonia (NH4). The NO2-reductase activity of membrane fractions was 2-fold higher than that of NO3-reductase consistent with the fact that NO3-reductase activity of M. avium cannot be detected if measured by nitrite formation. Membrane fractions of M. avium cells grown 1 week aerobically and then 2 weeks under anaerobic conditions had NO3-and NO2-reductase activities. Conclusion: The results are consistent with the presence of assimilatory NO3-and NO2-reductase activities in cells of M. avium grown under aerobic conditions. Further, the data suggest that a shift to anaerobic conditions results in the appearance of ammonium-insensitive NO3-and NO2-reductase activities; quite possibly that function in a dissimilatory role (redox balancing).http://www.ijmyco.org/article.asp?issn=2212-5531;year=2018;volume=7;issue=4;spage=328;epage=331;aulast=ButalaAmmoniumMycobacterium aviumnitratenitrate reductasenitritenitrite reductase |
| spellingShingle | Nitin S Butala Joseph Oliver Falkinham Nitrate and nitrite reductase activities of Mycobacterium avium International Journal of Mycobacteriology Ammonium Mycobacterium avium nitrate nitrate reductase nitrite nitrite reductase |
| title | Nitrate and nitrite reductase activities of Mycobacterium avium |
| title_full | Nitrate and nitrite reductase activities of Mycobacterium avium |
| title_fullStr | Nitrate and nitrite reductase activities of Mycobacterium avium |
| title_full_unstemmed | Nitrate and nitrite reductase activities of Mycobacterium avium |
| title_short | Nitrate and nitrite reductase activities of Mycobacterium avium |
| title_sort | nitrate and nitrite reductase activities of mycobacterium avium |
| topic | Ammonium Mycobacterium avium nitrate nitrate reductase nitrite nitrite reductase |
| url | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2018;volume=7;issue=4;spage=328;epage=331;aulast=Butala |
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