Secondary metabolites and antioxidant action of Croton heliotripifolius and Croton blanchetianus
Antioxidants are compounds that, at optimal concentrations in relation to oxidable substrates, react with free radicals preventing or reducing oxidative stress. Many diseases and degenerative processes are associated with overproduction of free radicals. This stimulated several research groups to in...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | Portuguese |
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Universidade Federal de Campina Grande
2017-09-01
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| Series: | Acta Brasiliensis |
| Online Access: | http://revistas.ufcg.edu.br/ActaBra/index.php/actabra/article/view/30 |
| _version_ | 1818993222989381632 |
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| author | Vitória Viviane Ferreira Aquino José Galberto Martins Costa Elissandra Couras Angélico Rosália Severo Medeiros Maria de Fatima Araujo Lucena Onaldo Guedes Rodrigues |
| author_facet | Vitória Viviane Ferreira Aquino José Galberto Martins Costa Elissandra Couras Angélico Rosália Severo Medeiros Maria de Fatima Araujo Lucena Onaldo Guedes Rodrigues |
| author_sort | Vitória Viviane Ferreira Aquino |
| collection | DOAJ |
| description | Antioxidants are compounds that, at optimal concentrations in relation to oxidable substrates, react with free radicals preventing or reducing oxidative stress. Many diseases and degenerative processes are associated with overproduction of free radicals. This stimulated several research groups to investigate the antioxidant potential of substances produced by several families of the world flora. This study identified the major classes of secondary metabolites present in ethanol leaf extracts of C. blanchetianus (Marmeleiro) and C. helliotripiifolius (Velame), and assessed their antioxidant potential. Ethanol extracts were obtained by the exhaustive extraction method cold and resulted in 3.29% and 3.26% extraction efficiency. Then phytochemical analyses were performed on the extracts to investigate the presence of tannins, flavonoids, flavonones, flavonols, catechins and xanthones. The antioxidant activity of extracts was determined by in vitro photocolorimetric method based on the sequestration of free radicals, using DPPH (1,1-diphenyl-2-picryl-hydrazyl). Both extracts were able to sequestrate DPPH free radical. Croton blanchetianus extract activity showed to be more efficient than BHT (Butylated Hidroxytoluene) (positive control), with EC50 = 6.5 ± 0.5 mg mL-1. Croton helliotripifolius extracts showed lower antioxidant activity, with EC50 = 50.15 ± 0.21 but still higher than that observed for BHT. However, the results observed for the ethanol extracts are possibly the result of the presence and concentration of phenolic compounds such as tannins and flavonoids, which were identified in this study. |
| first_indexed | 2024-12-20T20:38:38Z |
| format | Article |
| id | doaj.art-b662e9b392bc403a971b1a5820747881 |
| institution | Directory Open Access Journal |
| issn | 2526-432X 2526-4338 |
| language | Portuguese |
| last_indexed | 2024-12-20T20:38:38Z |
| publishDate | 2017-09-01 |
| publisher | Universidade Federal de Campina Grande |
| record_format | Article |
| series | Acta Brasiliensis |
| spelling | doaj.art-b662e9b392bc403a971b1a58207478812022-12-21T19:27:09ZporUniversidade Federal de Campina GrandeActa Brasiliensis2526-432X2526-43382017-09-0113283110.22571/Actabra1320173030Secondary metabolites and antioxidant action of Croton heliotripifolius and Croton blanchetianusVitória Viviane Ferreira Aquino0José Galberto Martins Costa1Elissandra Couras Angélico2Rosália Severo Medeiros3Maria de Fatima Araujo Lucena4Onaldo Guedes Rodrigues5Programa de Pós-Graduação em Ciência Animal, Universidade Federal de Campina Grande, Patos, Paraíba.Laboratório Multiusuário de Pesquisas Ambientais, Universidade Federal de Campina Grande, Patos, Paraíba.Laboratório de Pesquisa de Produtos Naturais, Crato, Ceará.Unidade Acadêmica de Ciências Biológicas, Universidade Federal de Campina Grande, Patos.Unidade Acadêmica de Ciências Biológicas, Universidade Federal de Campina Grande, Patos, 58700-971, Paraíba, Brasil.Programa de Pós-Graduação em Ciência Animal, Universidade Federal de Campina Grande, Patos, ParaíbaAntioxidants are compounds that, at optimal concentrations in relation to oxidable substrates, react with free radicals preventing or reducing oxidative stress. Many diseases and degenerative processes are associated with overproduction of free radicals. This stimulated several research groups to investigate the antioxidant potential of substances produced by several families of the world flora. This study identified the major classes of secondary metabolites present in ethanol leaf extracts of C. blanchetianus (Marmeleiro) and C. helliotripiifolius (Velame), and assessed their antioxidant potential. Ethanol extracts were obtained by the exhaustive extraction method cold and resulted in 3.29% and 3.26% extraction efficiency. Then phytochemical analyses were performed on the extracts to investigate the presence of tannins, flavonoids, flavonones, flavonols, catechins and xanthones. The antioxidant activity of extracts was determined by in vitro photocolorimetric method based on the sequestration of free radicals, using DPPH (1,1-diphenyl-2-picryl-hydrazyl). Both extracts were able to sequestrate DPPH free radical. Croton blanchetianus extract activity showed to be more efficient than BHT (Butylated Hidroxytoluene) (positive control), with EC50 = 6.5 ± 0.5 mg mL-1. Croton helliotripifolius extracts showed lower antioxidant activity, with EC50 = 50.15 ± 0.21 but still higher than that observed for BHT. However, the results observed for the ethanol extracts are possibly the result of the presence and concentration of phenolic compounds such as tannins and flavonoids, which were identified in this study.http://revistas.ufcg.edu.br/ActaBra/index.php/actabra/article/view/30 |
| spellingShingle | Vitória Viviane Ferreira Aquino José Galberto Martins Costa Elissandra Couras Angélico Rosália Severo Medeiros Maria de Fatima Araujo Lucena Onaldo Guedes Rodrigues Secondary metabolites and antioxidant action of Croton heliotripifolius and Croton blanchetianus Acta Brasiliensis |
| title | Secondary metabolites and antioxidant action of Croton heliotripifolius and Croton blanchetianus |
| title_full | Secondary metabolites and antioxidant action of Croton heliotripifolius and Croton blanchetianus |
| title_fullStr | Secondary metabolites and antioxidant action of Croton heliotripifolius and Croton blanchetianus |
| title_full_unstemmed | Secondary metabolites and antioxidant action of Croton heliotripifolius and Croton blanchetianus |
| title_short | Secondary metabolites and antioxidant action of Croton heliotripifolius and Croton blanchetianus |
| title_sort | secondary metabolites and antioxidant action of croton heliotripifolius and croton blanchetianus |
| url | http://revistas.ufcg.edu.br/ActaBra/index.php/actabra/article/view/30 |
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