A sterol-defined system for quantitative studies of sterol metabolism in C. elegans
Summary: This protocol describes the culturing of the nematode Caenorhabditis elegans (C. elegans) in a sterol-defined experimental system and the subsequent quantitative analysis of C. elegans sterols through gas chromatography-mass spectrometry. Although studied primarily in mammals, sterols are e...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721004172 |
| _version_ | 1819101708145393664 |
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| author | Benjamin Trabelcy Yoram Gerchman Amir Sapir |
| author_facet | Benjamin Trabelcy Yoram Gerchman Amir Sapir |
| author_sort | Benjamin Trabelcy |
| collection | DOAJ |
| description | Summary: This protocol describes the culturing of the nematode Caenorhabditis elegans (C. elegans) in a sterol-defined experimental system and the subsequent quantitative analysis of C. elegans sterols through gas chromatography-mass spectrometry. Although studied primarily in mammals, sterols are essential biomolecules for most eukaryotes. C. elegans cannot synthesize sterols and thus relies on the uptake of dietary sterols. Therefore, C. elegans is a powerful system to study metabolism in sterol-defined conditions that are described in our protocol.For complete details on the use and execution of this protocol, please refer to Shamsuzzama et al. (2020). |
| first_indexed | 2024-12-22T01:22:57Z |
| format | Article |
| id | doaj.art-b6663520a2fc4032acc6fba42d83e837 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-22T01:22:57Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-b6663520a2fc4032acc6fba42d83e8372022-12-21T18:43:42ZengElsevierSTAR Protocols2666-16672021-09-0123100710A sterol-defined system for quantitative studies of sterol metabolism in C. elegansBenjamin Trabelcy0Yoram Gerchman1Amir Sapir2Faculty of Natural Sciences, University of Haifa, Haifa 3498838 Israel; Corresponding authorFaculty of Natural Sciences, University of Haifa, Haifa 3498838 IsraelFaculty of Natural Sciences, University of Haifa, Haifa 3498838 Israel; Corresponding authorSummary: This protocol describes the culturing of the nematode Caenorhabditis elegans (C. elegans) in a sterol-defined experimental system and the subsequent quantitative analysis of C. elegans sterols through gas chromatography-mass spectrometry. Although studied primarily in mammals, sterols are essential biomolecules for most eukaryotes. C. elegans cannot synthesize sterols and thus relies on the uptake of dietary sterols. Therefore, C. elegans is a powerful system to study metabolism in sterol-defined conditions that are described in our protocol.For complete details on the use and execution of this protocol, please refer to Shamsuzzama et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166721004172GeneticsMass SpectrometryMetabolismModel Organisms |
| spellingShingle | Benjamin Trabelcy Yoram Gerchman Amir Sapir A sterol-defined system for quantitative studies of sterol metabolism in C. elegans STAR Protocols Genetics Mass Spectrometry Metabolism Model Organisms |
| title | A sterol-defined system for quantitative studies of sterol metabolism in C. elegans |
| title_full | A sterol-defined system for quantitative studies of sterol metabolism in C. elegans |
| title_fullStr | A sterol-defined system for quantitative studies of sterol metabolism in C. elegans |
| title_full_unstemmed | A sterol-defined system for quantitative studies of sterol metabolism in C. elegans |
| title_short | A sterol-defined system for quantitative studies of sterol metabolism in C. elegans |
| title_sort | sterol defined system for quantitative studies of sterol metabolism in c elegans |
| topic | Genetics Mass Spectrometry Metabolism Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721004172 |
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