Immunological characteristics of MAV/06 strain of varicella-zoster virus vaccine in an animal model
Abstract Background Varicella-zoster virus (VZV) is a pathogen that causes chickenpox and shingles in humans. Different types of the varicella vaccines derived from the Oka and MAV/06 strains are commercially available worldwide. Although the MAV/06 vaccine was introduced in 1990s, little was known...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-06-01
|
| Series: | BMC Immunology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12865-022-00503-6 |
| _version_ | 1828728026855112704 |
|---|---|
| author | Duckhyang Shin Younchul Shin Eunmi Kim Hyojung Nam Haiyan Nan Jaewoo Lee |
| author_facet | Duckhyang Shin Younchul Shin Eunmi Kim Hyojung Nam Haiyan Nan Jaewoo Lee |
| author_sort | Duckhyang Shin |
| collection | DOAJ |
| description | Abstract Background Varicella-zoster virus (VZV) is a pathogen that causes chickenpox and shingles in humans. Different types of the varicella vaccines derived from the Oka and MAV/06 strains are commercially available worldwide. Although the MAV/06 vaccine was introduced in 1990s, little was known about immunological characteristics. Results Here, we evaluated B and T cell immune response in animals inoculated with the Oka and MAV/06 vaccines as well as a new formulation of the MAV/06 vaccine. A variety of test methods were applied to evaluate T and B cell immune response. Plaque reduction neutralization test (PRNT) and fluorescent antibody to membrane antigen (FAMA) assay were conducted to measure the MAV/06 vaccine-induced antibody activity against various VZVs. Glycoprotein enzyme-linked immunosorbent assay (gpELISA) was used to compare the degree of the antibody responses induced by the two available commercial VZV vaccines and the MAV/06 vaccine. Interferon-gamma enzyme-linked immunosorbent spot (IFN-γ ELISpot) assays and cytokine bead array (CBA) assays were conducted to investigate T cell immune responses. Antibodies induced by MAV/06 vaccination showed immunogenicity against a variety of varicella-zoster virus and cross-reactivity among the virus clades. Conclusions It is indicating the similarity of the antibody responses induced by commercial varicella vaccines and the MAV/06 vaccine. Moreover, VZV-specific T cell immune response from MAV/06 vaccination was increased via Th1 cell response. MAV/06 varicella vaccine induced both humoral and cellular immune response via Th1 cell mediated response. |
| first_indexed | 2024-04-12T14:08:55Z |
| format | Article |
| id | doaj.art-b667dfbbae9e4808a3b87e6b6b83434c |
| institution | Directory Open Access Journal |
| issn | 1471-2172 |
| language | English |
| last_indexed | 2024-04-12T14:08:55Z |
| publishDate | 2022-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Immunology |
| spelling | doaj.art-b667dfbbae9e4808a3b87e6b6b83434c2022-12-22T03:29:59ZengBMCBMC Immunology1471-21722022-06-012311910.1186/s12865-022-00503-6Immunological characteristics of MAV/06 strain of varicella-zoster virus vaccine in an animal modelDuckhyang Shin0Younchul Shin1Eunmi Kim2Hyojung Nam3Haiyan Nan4Jaewoo Lee5GC Biopharma Corp.GC Biopharma Corp.MOGAM Institute for Biomedical ResearchGC Biopharma Corp.GC Biopharma Corp.GC Biopharma Corp.Abstract Background Varicella-zoster virus (VZV) is a pathogen that causes chickenpox and shingles in humans. Different types of the varicella vaccines derived from the Oka and MAV/06 strains are commercially available worldwide. Although the MAV/06 vaccine was introduced in 1990s, little was known about immunological characteristics. Results Here, we evaluated B and T cell immune response in animals inoculated with the Oka and MAV/06 vaccines as well as a new formulation of the MAV/06 vaccine. A variety of test methods were applied to evaluate T and B cell immune response. Plaque reduction neutralization test (PRNT) and fluorescent antibody to membrane antigen (FAMA) assay were conducted to measure the MAV/06 vaccine-induced antibody activity against various VZVs. Glycoprotein enzyme-linked immunosorbent assay (gpELISA) was used to compare the degree of the antibody responses induced by the two available commercial VZV vaccines and the MAV/06 vaccine. Interferon-gamma enzyme-linked immunosorbent spot (IFN-γ ELISpot) assays and cytokine bead array (CBA) assays were conducted to investigate T cell immune responses. Antibodies induced by MAV/06 vaccination showed immunogenicity against a variety of varicella-zoster virus and cross-reactivity among the virus clades. Conclusions It is indicating the similarity of the antibody responses induced by commercial varicella vaccines and the MAV/06 vaccine. Moreover, VZV-specific T cell immune response from MAV/06 vaccination was increased via Th1 cell response. MAV/06 varicella vaccine induced both humoral and cellular immune response via Th1 cell mediated response.https://doi.org/10.1186/s12865-022-00503-6Varicella zoster virusMAV/06 vaccineSerological cross-reactivityCellular immune response |
| spellingShingle | Duckhyang Shin Younchul Shin Eunmi Kim Hyojung Nam Haiyan Nan Jaewoo Lee Immunological characteristics of MAV/06 strain of varicella-zoster virus vaccine in an animal model BMC Immunology Varicella zoster virus MAV/06 vaccine Serological cross-reactivity Cellular immune response |
| title | Immunological characteristics of MAV/06 strain of varicella-zoster virus vaccine in an animal model |
| title_full | Immunological characteristics of MAV/06 strain of varicella-zoster virus vaccine in an animal model |
| title_fullStr | Immunological characteristics of MAV/06 strain of varicella-zoster virus vaccine in an animal model |
| title_full_unstemmed | Immunological characteristics of MAV/06 strain of varicella-zoster virus vaccine in an animal model |
| title_short | Immunological characteristics of MAV/06 strain of varicella-zoster virus vaccine in an animal model |
| title_sort | immunological characteristics of mav 06 strain of varicella zoster virus vaccine in an animal model |
| topic | Varicella zoster virus MAV/06 vaccine Serological cross-reactivity Cellular immune response |
| url | https://doi.org/10.1186/s12865-022-00503-6 |
| work_keys_str_mv | AT duckhyangshin immunologicalcharacteristicsofmav06strainofvaricellazostervirusvaccineinananimalmodel AT younchulshin immunologicalcharacteristicsofmav06strainofvaricellazostervirusvaccineinananimalmodel AT eunmikim immunologicalcharacteristicsofmav06strainofvaricellazostervirusvaccineinananimalmodel AT hyojungnam immunologicalcharacteristicsofmav06strainofvaricellazostervirusvaccineinananimalmodel AT haiyannan immunologicalcharacteristicsofmav06strainofvaricellazostervirusvaccineinananimalmodel AT jaewoolee immunologicalcharacteristicsofmav06strainofvaricellazostervirusvaccineinananimalmodel |