Rapid and Sensitive Detection of BLAD in Cattle Population
Bovine leukocyte adhesion deficiency (BLAD) is an autosomal recessive disorder with negative impact on dairy cattle breeding. The molecular basis of BLAD is a single point mutation (A→G), resulting in a single amino acid substitution (aspartic acid → glycine) at amino acid 128 in the adhesion molecu...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-09-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | https://spasb.ro/index.php/public_html/article/view/534 |
| _version_ | 1797214976279052288 |
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| author | Daniela Elena Ilie Ada Cean Oana Isabella Gavriliuc Nicolae Păcală |
| author_facet | Daniela Elena Ilie Ada Cean Oana Isabella Gavriliuc Nicolae Păcală |
| author_sort | Daniela Elena Ilie |
| collection | DOAJ |
| description | Bovine leukocyte adhesion deficiency (BLAD) is an autosomal recessive disorder with negative impact on dairy cattle breeding. The molecular basis of BLAD is a single point mutation (A→G), resulting in a single amino acid substitution (aspartic acid → glycine) at amino acid 128 in the adhesion molecule CD18. The object of this study was to establish a fast and sensitive molecular genotyping assay to detect BLAD carriers using high-resolution melting (HRM) curve analysis. We tested animals with known genotypes for BLAD that were previously confirmed by PCR-RFLP method, and then examined the sensitivity of mutation detection using PCR followed by HRM curve analysis. BLAD carriers were readily detectable using HRM assay. Thus, the PCR-HRM genotyping method is a rapid, easily interpretable, reliable and cost-effective assay for BLAD mutant allele detection. This assay can be useful in cattle genotyping and genetic selection. |
| first_indexed | 2024-04-24T11:22:43Z |
| format | Article |
| id | doaj.art-b669d591df1649b9bcf3891af8fabde9 |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:22:43Z |
| publishDate | 2023-09-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-b669d591df1649b9bcf3891af8fabde92024-04-11T04:29:15ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-09-01471109109534Rapid and Sensitive Detection of BLAD in Cattle PopulationDaniela Elena Ilie0Ada Cean1Oana Isabella Gavriliuc2Nicolae Păcală3Research and Development Station for Bovine - Arad, 310059, Arad, Bodrogului 32, RomaniaResearch and Development Station for Bovine - Arad, 310059, Arad, Bodrogului 32, RomaniaUniversity of Medicine and Pharmacy "Victor Babes" Timisoara, 300041, Eftimie Murgu 2, RomaniaBanat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara, Faculty of Animal Science and Biotechnologies, 300645, Timisoara, Calea Aradului 119, RomaniaBovine leukocyte adhesion deficiency (BLAD) is an autosomal recessive disorder with negative impact on dairy cattle breeding. The molecular basis of BLAD is a single point mutation (A→G), resulting in a single amino acid substitution (aspartic acid → glycine) at amino acid 128 in the adhesion molecule CD18. The object of this study was to establish a fast and sensitive molecular genotyping assay to detect BLAD carriers using high-resolution melting (HRM) curve analysis. We tested animals with known genotypes for BLAD that were previously confirmed by PCR-RFLP method, and then examined the sensitivity of mutation detection using PCR followed by HRM curve analysis. BLAD carriers were readily detectable using HRM assay. Thus, the PCR-HRM genotyping method is a rapid, easily interpretable, reliable and cost-effective assay for BLAD mutant allele detection. This assay can be useful in cattle genotyping and genetic selection.https://spasb.ro/index.php/public_html/article/view/534bladcd18genotypinghigh-resolution melting |
| spellingShingle | Daniela Elena Ilie Ada Cean Oana Isabella Gavriliuc Nicolae Păcală Rapid and Sensitive Detection of BLAD in Cattle Population Scientific Papers Animal Science and Biotechnologies blad cd18 genotyping high-resolution melting |
| title | Rapid and Sensitive Detection of BLAD in Cattle Population |
| title_full | Rapid and Sensitive Detection of BLAD in Cattle Population |
| title_fullStr | Rapid and Sensitive Detection of BLAD in Cattle Population |
| title_full_unstemmed | Rapid and Sensitive Detection of BLAD in Cattle Population |
| title_short | Rapid and Sensitive Detection of BLAD in Cattle Population |
| title_sort | rapid and sensitive detection of blad in cattle population |
| topic | blad cd18 genotyping high-resolution melting |
| url | https://spasb.ro/index.php/public_html/article/view/534 |
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