Bioactive Compounds from <i>P. pertomentellum</i> That Regulate QS, Biofilm Formation and Virulence Factor Production of <i>P. aeruginosa</i>
<i>Pseudomonas aeruginosa</i> is an opportunistic pathogen responsible for many nosocomial infections. This bacterium uses Quorum Sensing (QS) to generate antimicrobial resistance (AMR) so its disruption is considered a novel approach. The current study describes the antibiofilm and QS i...
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MDPI AG
2023-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/17/6181 |
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| author | Lida V. Hernández-Moreno Ludy C. Pabón-Baquero Juliet A. Prieto-Rodriguez Oscar J. Patiño-Ladino |
| author_facet | Lida V. Hernández-Moreno Ludy C. Pabón-Baquero Juliet A. Prieto-Rodriguez Oscar J. Patiño-Ladino |
| author_sort | Lida V. Hernández-Moreno |
| collection | DOAJ |
| description | <i>Pseudomonas aeruginosa</i> is an opportunistic pathogen responsible for many nosocomial infections. This bacterium uses Quorum Sensing (QS) to generate antimicrobial resistance (AMR) so its disruption is considered a novel approach. The current study describes the antibiofilm and QS inhibitory potential of extract and chemical components from <i>Piper pertomentellum</i>. The methodo- logy included the phytochemical study on the aerial part of the species, the determination of QS inhibition efficacy on <i>Chromobacterium violaceum</i> and the evaluation of the effect on biofilm formation and virulence factors on <i>P. aeruginosa</i>. The phytochemical study led to the isolation and identification of a new piperamide (ethyltembamide <b>1</b>), together with four known amides (tembamide acetate <b>2</b>, cepharadione B <b>3</b>, benzamide <b>4</b> and tembamide <b>5</b>). The results indicated that the ethanolic extract and some fractions reduced violacein production in <i>C. violaceum</i>, however, only the ethanolic extract caused inhibition of biofilm formation of <i>P. aeruginosa</i> on polystyrene microtiter plates. Finally, the investigation determined that molecules (<b>1</b>–<b>5</b>) inhibited the formation of biofilms (50% approximately), while compounds <b>2</b>–<b>4</b> can inhibit pyocyanin and elastase production (30–50% approximately). In this way, the study contributes to the determination of the potential of extract and chemical constituents from <i>P pertomentellum</i> to regulate the QS system in <i>P. aeruginosa</i>. |
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| language | English |
| last_indexed | 2024-03-10T23:17:40Z |
| publishDate | 2023-08-01 |
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| series | Molecules |
| spelling | doaj.art-b66a5bb4172d4465a46e125e7cdb69e72023-11-19T08:32:26ZengMDPI AGMolecules1420-30492023-08-012817618110.3390/molecules28176181Bioactive Compounds from <i>P. pertomentellum</i> That Regulate QS, Biofilm Formation and Virulence Factor Production of <i>P. aeruginosa</i>Lida V. Hernández-Moreno0Ludy C. Pabón-Baquero1Juliet A. Prieto-Rodriguez2Oscar J. Patiño-Ladino3Departamento de Química, Facultad de Ciencias, Universidad Nacional de Colombia, Sede Bogotá, Bogotá 111321, ColombiaEscuela de Ciencias Básicas y Aplicadas, Universidad de La Salle, Bogotá 111711, ColombiaDepartamento de Química, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá 110231, ColombiaDepartamento de Química, Facultad de Ciencias, Universidad Nacional de Colombia, Sede Bogotá, Bogotá 111321, Colombia<i>Pseudomonas aeruginosa</i> is an opportunistic pathogen responsible for many nosocomial infections. This bacterium uses Quorum Sensing (QS) to generate antimicrobial resistance (AMR) so its disruption is considered a novel approach. The current study describes the antibiofilm and QS inhibitory potential of extract and chemical components from <i>Piper pertomentellum</i>. The methodo- logy included the phytochemical study on the aerial part of the species, the determination of QS inhibition efficacy on <i>Chromobacterium violaceum</i> and the evaluation of the effect on biofilm formation and virulence factors on <i>P. aeruginosa</i>. The phytochemical study led to the isolation and identification of a new piperamide (ethyltembamide <b>1</b>), together with four known amides (tembamide acetate <b>2</b>, cepharadione B <b>3</b>, benzamide <b>4</b> and tembamide <b>5</b>). The results indicated that the ethanolic extract and some fractions reduced violacein production in <i>C. violaceum</i>, however, only the ethanolic extract caused inhibition of biofilm formation of <i>P. aeruginosa</i> on polystyrene microtiter plates. Finally, the investigation determined that molecules (<b>1</b>–<b>5</b>) inhibited the formation of biofilms (50% approximately), while compounds <b>2</b>–<b>4</b> can inhibit pyocyanin and elastase production (30–50% approximately). In this way, the study contributes to the determination of the potential of extract and chemical constituents from <i>P pertomentellum</i> to regulate the QS system in <i>P. aeruginosa</i>.https://www.mdpi.com/1420-3049/28/17/6181<i>Piper pertomentellum</i>amides<i>Pseudomonas aeruginosa</i>virulence factorsbiofilmquorum sensing |
| spellingShingle | Lida V. Hernández-Moreno Ludy C. Pabón-Baquero Juliet A. Prieto-Rodriguez Oscar J. Patiño-Ladino Bioactive Compounds from <i>P. pertomentellum</i> That Regulate QS, Biofilm Formation and Virulence Factor Production of <i>P. aeruginosa</i> Molecules <i>Piper pertomentellum</i> amides <i>Pseudomonas aeruginosa</i> virulence factors biofilm quorum sensing |
| title | Bioactive Compounds from <i>P. pertomentellum</i> That Regulate QS, Biofilm Formation and Virulence Factor Production of <i>P. aeruginosa</i> |
| title_full | Bioactive Compounds from <i>P. pertomentellum</i> That Regulate QS, Biofilm Formation and Virulence Factor Production of <i>P. aeruginosa</i> |
| title_fullStr | Bioactive Compounds from <i>P. pertomentellum</i> That Regulate QS, Biofilm Formation and Virulence Factor Production of <i>P. aeruginosa</i> |
| title_full_unstemmed | Bioactive Compounds from <i>P. pertomentellum</i> That Regulate QS, Biofilm Formation and Virulence Factor Production of <i>P. aeruginosa</i> |
| title_short | Bioactive Compounds from <i>P. pertomentellum</i> That Regulate QS, Biofilm Formation and Virulence Factor Production of <i>P. aeruginosa</i> |
| title_sort | bioactive compounds from i p pertomentellum i that regulate qs biofilm formation and virulence factor production of i p aeruginosa i |
| topic | <i>Piper pertomentellum</i> amides <i>Pseudomonas aeruginosa</i> virulence factors biofilm quorum sensing |
| url | https://www.mdpi.com/1420-3049/28/17/6181 |
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