Raman Microspectrometry: An Alternative Method of Age Estimation from Dentin and Cementum
Introduction: Raman spectroscopy is simple, quick, sensitive and non destructive form of tissue examination that provides vital data about the structure, molecular composition and interactions within a sample. The human hard tissues like teeth and bone are able to resist decay for long even after ot...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2017-10-01
|
| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | http://jcdr.net/articles/PDF/10705/31161_CE(Ra1)_F(SS)PF1-(SY_MJ_PY)_PFA(MJ_GG).pdf |
| _version_ | 1819169873334370304 |
|---|---|
| author | Karuna Kumari Roopa S Rao Sachin Chakradhar Sarode Gargi Sachin Sarode Shankargouda Patil |
| author_facet | Karuna Kumari Roopa S Rao Sachin Chakradhar Sarode Gargi Sachin Sarode Shankargouda Patil |
| author_sort | Karuna Kumari |
| collection | DOAJ |
| description | Introduction: Raman spectroscopy is simple, quick, sensitive and non destructive form of tissue examination that provides vital data about the structure, molecular composition and interactions within a sample. The human hard tissues like teeth and bone are able to resist decay for long even after other tissues are lost, thus have valuable forensic importance.
Aim: To ascertain the known age of the teeth by analysing dentin and cementum using Raman microspectrometry and assess the accuracy of age estimation by comparison of dentin with cementum.
Materials and Methods: The sound permanent extracted tooth specimens (40) of age ranging between 12-74 years were collected and sectioned longitudinally and different dentinal and cemental areas were analysed by Raman microspectrometry. The spectra of dentin and cementum were used as predictors of age estimation. For each sample, ratios were obtained for dentin and cementum areas, and Pearson’s correlation coefficient was calculated. Ratios, which had a correlation coefficient greater than 0.40 were used for further statistical analysis. This led to selection of ratios only for dentin areas, and it allowed us to develop a regression formula. Partial Least Square (PLS) regression method was used for computing our model.
Results: A significant correlation was observed between the actual chronological age and predicted age of the individual using dentinal areas of the tooth. A closest to the estimated result was achieved, with an error of three years between predicted and actual chorological age.
Conclusion: Raman microspectrometry may be considered as an alternative to the conventional method of age estimation and contribute to the identification of individuals. |
| first_indexed | 2024-12-22T19:26:25Z |
| format | Article |
| id | doaj.art-b66bbae081a14778bb618fa1e42bae81 |
| institution | Directory Open Access Journal |
| issn | 0973-709X 2249-782X |
| language | English |
| last_indexed | 2024-12-22T19:26:25Z |
| publishDate | 2017-10-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-b66bbae081a14778bb618fa1e42bae812022-12-21T18:15:14ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research0973-709X2249-782X2017-10-011110ZC11ZC1610.7860/JCDR/2017/31161.10705Raman Microspectrometry: An Alternative Method of Age Estimation from Dentin and CementumKaruna Kumari0Roopa S Rao1Sachin Chakradhar Sarode2Gargi Sachin Sarode3Shankargouda Patil4Postgraduate Student, Department of Oral Pathology and Microbiology, Faculty of Dental Sciences, MS Ramaiah University of Applied Sciences, Bengaluru, Karnataka, India.Professor and Head, Department of Oral Pathology and Microbiology, Faculty of Dental Sciences, MS Ramaiah University of Applied Sciences, Bengaluru, Karnataka, India.Professor, Department of Oral Pathology and Microbiology, Dr. D. Y. Patil Dental College and Hospital, Dr. D. Y. Patil Vidyapeeth, Pune, Maharashtra, India.Professor, Department of Oral Pathology and Microbiology, Dr. D. Y. Patil Dental College and Hospital, Dr. D. Y. Patil Vidyapeeth, Pune, Maharashtra, India.Associate Professor, Department of Oral and Maxillofacial Surgery, Division of Oral Pathology, College of Dentistry, Jazan University, Jazan, Kingdom of Saudi Arabia.Introduction: Raman spectroscopy is simple, quick, sensitive and non destructive form of tissue examination that provides vital data about the structure, molecular composition and interactions within a sample. The human hard tissues like teeth and bone are able to resist decay for long even after other tissues are lost, thus have valuable forensic importance. Aim: To ascertain the known age of the teeth by analysing dentin and cementum using Raman microspectrometry and assess the accuracy of age estimation by comparison of dentin with cementum. Materials and Methods: The sound permanent extracted tooth specimens (40) of age ranging between 12-74 years were collected and sectioned longitudinally and different dentinal and cemental areas were analysed by Raman microspectrometry. The spectra of dentin and cementum were used as predictors of age estimation. For each sample, ratios were obtained for dentin and cementum areas, and Pearson’s correlation coefficient was calculated. Ratios, which had a correlation coefficient greater than 0.40 were used for further statistical analysis. This led to selection of ratios only for dentin areas, and it allowed us to develop a regression formula. Partial Least Square (PLS) regression method was used for computing our model. Results: A significant correlation was observed between the actual chronological age and predicted age of the individual using dentinal areas of the tooth. A closest to the estimated result was achieved, with an error of three years between predicted and actual chorological age. Conclusion: Raman microspectrometry may be considered as an alternative to the conventional method of age estimation and contribute to the identification of individuals.http://jcdr.net/articles/PDF/10705/31161_CE(Ra1)_F(SS)PF1-(SY_MJ_PY)_PFA(MJ_GG).pdfChronological ageForensic dentistryForensic odontology |
| spellingShingle | Karuna Kumari Roopa S Rao Sachin Chakradhar Sarode Gargi Sachin Sarode Shankargouda Patil Raman Microspectrometry: An Alternative Method of Age Estimation from Dentin and Cementum Journal of Clinical and Diagnostic Research Chronological age Forensic dentistry Forensic odontology |
| title | Raman Microspectrometry: An Alternative Method of Age Estimation from Dentin and Cementum |
| title_full | Raman Microspectrometry: An Alternative Method of Age Estimation from Dentin and Cementum |
| title_fullStr | Raman Microspectrometry: An Alternative Method of Age Estimation from Dentin and Cementum |
| title_full_unstemmed | Raman Microspectrometry: An Alternative Method of Age Estimation from Dentin and Cementum |
| title_short | Raman Microspectrometry: An Alternative Method of Age Estimation from Dentin and Cementum |
| title_sort | raman microspectrometry an alternative method of age estimation from dentin and cementum |
| topic | Chronological age Forensic dentistry Forensic odontology |
| url | http://jcdr.net/articles/PDF/10705/31161_CE(Ra1)_F(SS)PF1-(SY_MJ_PY)_PFA(MJ_GG).pdf |
| work_keys_str_mv | AT karunakumari ramanmicrospectrometryanalternativemethodofageestimationfromdentinandcementum AT roopasrao ramanmicrospectrometryanalternativemethodofageestimationfromdentinandcementum AT sachinchakradharsarode ramanmicrospectrometryanalternativemethodofageestimationfromdentinandcementum AT gargisachinsarode ramanmicrospectrometryanalternativemethodofageestimationfromdentinandcementum AT shankargoudapatil ramanmicrospectrometryanalternativemethodofageestimationfromdentinandcementum |