<i>GhAGL16</i> (<i>AGAMOUS</i>-<i>LIKE16</i>) Negatively Regulates Tolerance to Water Deficit in Transgenic <i>Arabidopsis</i> and Cotton
Cotton is one of the most economically important crops in the world, and drought is a key abiotic factor that can significantly reduce cotton yield. MADS-box transcription factors play essential roles in various aspects of plant growth and development as well as responses to biotic and abiotic stres...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/13/2/282 |
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| author | Jianfeng Lei Yangzi You Peihong Dai Li Yu Yue Li Chao Liu Xiaodong Liu |
| author_facet | Jianfeng Lei Yangzi You Peihong Dai Li Yu Yue Li Chao Liu Xiaodong Liu |
| author_sort | Jianfeng Lei |
| collection | DOAJ |
| description | Cotton is one of the most economically important crops in the world, and drought is a key abiotic factor that can significantly reduce cotton yield. MADS-box transcription factors play essential roles in various aspects of plant growth and development as well as responses to biotic and abiotic stress. However, the use of MADS-box transcription factors to regulate water stress responses has not been fully explored in cotton. Here, we showed that GhAGL16 acts as a negative regulator of water deficit in cotton, at least in part by regulating ABA signaling. <i>GhAGL16</i>-overexpressing (<i>GhAGL16</i>-OE) transgenic <i>Arabidopsis</i> had lower survival rates and relative water contents (RWCs) under water stress. Isolated leaves of <i>GhAGL16</i>-OE <i>Arabidopsis</i> had increased water loss rates, likely attributable to their increased stomatal density. <i>GhAGL16</i>-OE <i>Arabidopsis</i> also showed reduced primary root lengths in response to mannitol treatment and decreased sensitivity of seed germination to ABA treatment. By contrast, silencing <i>GhAGL16</i> in cotton enhanced tolerance to water deficit by increasing proline (Pro) content, increasing superoxide dismutase (SOD) and peroxidase (POD) activities, and reducing malondialdehyde (MDA) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) contents under water stress. Subcellular localization and transcriptional activation assays confirmed that GhAGL16 is a nuclear protein that lacks transcriptional self-activation activity. The expression of ABA biosynthesis-related genes (<i>GhNCED3/7/14</i>), a catabolism-related gene (<i>GhCYP707A</i>), and a gene related to the ABA signaling pathway (<i>GhABF4</i>) was altered in <i>GhAGL16</i>-silenced plants. Taken together, our data demonstrate that GhAGL16 plays an important role in cotton resistance to water stress. |
| first_indexed | 2024-03-08T09:48:01Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-08T09:48:01Z |
| publishDate | 2024-01-01 |
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| spelling | doaj.art-b66c50a44cf247b4bd263039b0af4de82024-01-29T14:11:49ZengMDPI AGPlants2223-77472024-01-0113228210.3390/plants13020282<i>GhAGL16</i> (<i>AGAMOUS</i>-<i>LIKE16</i>) Negatively Regulates Tolerance to Water Deficit in Transgenic <i>Arabidopsis</i> and CottonJianfeng Lei0Yangzi You1Peihong Dai2Li Yu3Yue Li4Chao Liu5Xiaodong Liu6College of Agronomy, Xinjiang Agricultural University, Nongda East Road, Urumqi 830052, ChinaCollege of Life Sciences, Xinjiang Agricultural University, Nongda East Road, Urumqi 830052, ChinaCollege of Life Sciences, Xinjiang Agricultural University, Nongda East Road, Urumqi 830052, ChinaCollege of Life Sciences, Xinjiang Agricultural University, Nongda East Road, Urumqi 830052, ChinaCollege of Life Sciences, Xinjiang Agricultural University, Nongda East Road, Urumqi 830052, ChinaCollege of Life Sciences, Xinjiang Agricultural University, Nongda East Road, Urumqi 830052, ChinaCollege of Life Sciences, Xinjiang Agricultural University, Nongda East Road, Urumqi 830052, ChinaCotton is one of the most economically important crops in the world, and drought is a key abiotic factor that can significantly reduce cotton yield. MADS-box transcription factors play essential roles in various aspects of plant growth and development as well as responses to biotic and abiotic stress. However, the use of MADS-box transcription factors to regulate water stress responses has not been fully explored in cotton. Here, we showed that GhAGL16 acts as a negative regulator of water deficit in cotton, at least in part by regulating ABA signaling. <i>GhAGL16</i>-overexpressing (<i>GhAGL16</i>-OE) transgenic <i>Arabidopsis</i> had lower survival rates and relative water contents (RWCs) under water stress. Isolated leaves of <i>GhAGL16</i>-OE <i>Arabidopsis</i> had increased water loss rates, likely attributable to their increased stomatal density. <i>GhAGL16</i>-OE <i>Arabidopsis</i> also showed reduced primary root lengths in response to mannitol treatment and decreased sensitivity of seed germination to ABA treatment. By contrast, silencing <i>GhAGL16</i> in cotton enhanced tolerance to water deficit by increasing proline (Pro) content, increasing superoxide dismutase (SOD) and peroxidase (POD) activities, and reducing malondialdehyde (MDA) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) contents under water stress. Subcellular localization and transcriptional activation assays confirmed that GhAGL16 is a nuclear protein that lacks transcriptional self-activation activity. The expression of ABA biosynthesis-related genes (<i>GhNCED3/7/14</i>), a catabolism-related gene (<i>GhCYP707A</i>), and a gene related to the ABA signaling pathway (<i>GhABF4</i>) was altered in <i>GhAGL16</i>-silenced plants. Taken together, our data demonstrate that GhAGL16 plays an important role in cotton resistance to water stress.https://www.mdpi.com/2223-7747/13/2/282MADS-box transcription factor<i>GhAGL16</i>water stressstomataABA signaling |
| spellingShingle | Jianfeng Lei Yangzi You Peihong Dai Li Yu Yue Li Chao Liu Xiaodong Liu <i>GhAGL16</i> (<i>AGAMOUS</i>-<i>LIKE16</i>) Negatively Regulates Tolerance to Water Deficit in Transgenic <i>Arabidopsis</i> and Cotton Plants MADS-box transcription factor <i>GhAGL16</i> water stress stomata ABA signaling |
| title | <i>GhAGL16</i> (<i>AGAMOUS</i>-<i>LIKE16</i>) Negatively Regulates Tolerance to Water Deficit in Transgenic <i>Arabidopsis</i> and Cotton |
| title_full | <i>GhAGL16</i> (<i>AGAMOUS</i>-<i>LIKE16</i>) Negatively Regulates Tolerance to Water Deficit in Transgenic <i>Arabidopsis</i> and Cotton |
| title_fullStr | <i>GhAGL16</i> (<i>AGAMOUS</i>-<i>LIKE16</i>) Negatively Regulates Tolerance to Water Deficit in Transgenic <i>Arabidopsis</i> and Cotton |
| title_full_unstemmed | <i>GhAGL16</i> (<i>AGAMOUS</i>-<i>LIKE16</i>) Negatively Regulates Tolerance to Water Deficit in Transgenic <i>Arabidopsis</i> and Cotton |
| title_short | <i>GhAGL16</i> (<i>AGAMOUS</i>-<i>LIKE16</i>) Negatively Regulates Tolerance to Water Deficit in Transgenic <i>Arabidopsis</i> and Cotton |
| title_sort | i ghagl16 i i agamous i i like16 i negatively regulates tolerance to water deficit in transgenic i arabidopsis i and cotton |
| topic | MADS-box transcription factor <i>GhAGL16</i> water stress stomata ABA signaling |
| url | https://www.mdpi.com/2223-7747/13/2/282 |
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