Investigation of Changes in Spermatozoon Characteristics, Chromatin Structure, and Antioxidant/Oxidant Parameters after Freeze-Thawing of Hesperidin (Vitamin P) Doses Added to Ram Semen
We conducted this study to determine the potential cryopreservative effects of different hesperidin (vitamin P; H) doses on ram semen after freeze-thawing. Semen samples were obtained from Sönmez rams using an artificial vagina. The samples were divided into six groups: control, 10, 50, 100, 250, an...
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MDPI AG
2022-11-01
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| Series: | Life |
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| author | Deniz Yeni Şükrü Güngör Fatih Avdatek Mehmet Fuat Gülhan Kemal Tuna Olğaç Muhammed Enes İnanç Barış Denk Umut Taşdemir |
| author_facet | Deniz Yeni Şükrü Güngör Fatih Avdatek Mehmet Fuat Gülhan Kemal Tuna Olğaç Muhammed Enes İnanç Barış Denk Umut Taşdemir |
| author_sort | Deniz Yeni |
| collection | DOAJ |
| description | We conducted this study to determine the potential cryopreservative effects of different hesperidin (vitamin P; H) doses on ram semen after freeze-thawing. Semen samples were obtained from Sönmez rams using an artificial vagina. The samples were divided into six groups: control, 10, 50, 100, 250, and 500 µg/mL H (C, H10, H50, H100, H250, and H500, respectively). At the end of the study, sperm motility and kinetic parameters, acrosome integrity (AI), mitochondrial membrane potential (MMP), viability, lipid peroxidation levels (LPL), chromatin damage, oxidant parameters, and antioxidant parameters were assayed. None of the doses of H added to the semen extender showed any enhancing effects on progressive motility compared to C (<i>p</i> > 0.05). In fact, H500 had negative effects (<i>p</i> < 0.05). Moreover, AI was the highest at the H10 dose, while LPL values were the lowest at the same dose (<i>p</i> < 0.05). The doses of H10 and H50 added to the Tris extender medium showed positive effects on sperm cell chromatin damage. Consequently, we can say that H doses used in this study are not effective on semen progressive motility, but the H10 dose is effective on AI and chromatin damage by reducing LPL. |
| first_indexed | 2024-03-09T18:55:05Z |
| format | Article |
| id | doaj.art-b670b42193f24a8e90b93dd4a9429c95 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T18:55:05Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-b670b42193f24a8e90b93dd4a9429c952023-11-24T05:30:50ZengMDPI AGLife2075-17292022-11-011211178010.3390/life12111780Investigation of Changes in Spermatozoon Characteristics, Chromatin Structure, and Antioxidant/Oxidant Parameters after Freeze-Thawing of Hesperidin (Vitamin P) Doses Added to Ram SemenDeniz Yeni0Şükrü Güngör1Fatih Avdatek2Mehmet Fuat Gülhan3Kemal Tuna Olğaç4Muhammed Enes İnanç5Barış Denk6Umut Taşdemir7Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar 03200, TurkeyDepartment of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur 15030, TurkeyDepartment of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar 03200, TurkeyDepartment of Medicinal and Aromatic Plants, Vocational School of Technical Sciences, Aksaray University, Aksaray 68100, TurkeyDepartment of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Ankara University, Ankara 06560, TurkeyDepartment of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur 15030, TurkeyDepartment of Biochemistry, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisa 03200, TurkeyDepartment of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Aksaray University, Aksaray 68100, TurkeyWe conducted this study to determine the potential cryopreservative effects of different hesperidin (vitamin P; H) doses on ram semen after freeze-thawing. Semen samples were obtained from Sönmez rams using an artificial vagina. The samples were divided into six groups: control, 10, 50, 100, 250, and 500 µg/mL H (C, H10, H50, H100, H250, and H500, respectively). At the end of the study, sperm motility and kinetic parameters, acrosome integrity (AI), mitochondrial membrane potential (MMP), viability, lipid peroxidation levels (LPL), chromatin damage, oxidant parameters, and antioxidant parameters were assayed. None of the doses of H added to the semen extender showed any enhancing effects on progressive motility compared to C (<i>p</i> > 0.05). In fact, H500 had negative effects (<i>p</i> < 0.05). Moreover, AI was the highest at the H10 dose, while LPL values were the lowest at the same dose (<i>p</i> < 0.05). The doses of H10 and H50 added to the Tris extender medium showed positive effects on sperm cell chromatin damage. Consequently, we can say that H doses used in this study are not effective on semen progressive motility, but the H10 dose is effective on AI and chromatin damage by reducing LPL.https://www.mdpi.com/2075-1729/12/11/1780semenhesperidincryopreservationoxidative stressantioxidantram |
| spellingShingle | Deniz Yeni Şükrü Güngör Fatih Avdatek Mehmet Fuat Gülhan Kemal Tuna Olğaç Muhammed Enes İnanç Barış Denk Umut Taşdemir Investigation of Changes in Spermatozoon Characteristics, Chromatin Structure, and Antioxidant/Oxidant Parameters after Freeze-Thawing of Hesperidin (Vitamin P) Doses Added to Ram Semen Life semen hesperidin cryopreservation oxidative stress antioxidant ram |
| title | Investigation of Changes in Spermatozoon Characteristics, Chromatin Structure, and Antioxidant/Oxidant Parameters after Freeze-Thawing of Hesperidin (Vitamin P) Doses Added to Ram Semen |
| title_full | Investigation of Changes in Spermatozoon Characteristics, Chromatin Structure, and Antioxidant/Oxidant Parameters after Freeze-Thawing of Hesperidin (Vitamin P) Doses Added to Ram Semen |
| title_fullStr | Investigation of Changes in Spermatozoon Characteristics, Chromatin Structure, and Antioxidant/Oxidant Parameters after Freeze-Thawing of Hesperidin (Vitamin P) Doses Added to Ram Semen |
| title_full_unstemmed | Investigation of Changes in Spermatozoon Characteristics, Chromatin Structure, and Antioxidant/Oxidant Parameters after Freeze-Thawing of Hesperidin (Vitamin P) Doses Added to Ram Semen |
| title_short | Investigation of Changes in Spermatozoon Characteristics, Chromatin Structure, and Antioxidant/Oxidant Parameters after Freeze-Thawing of Hesperidin (Vitamin P) Doses Added to Ram Semen |
| title_sort | investigation of changes in spermatozoon characteristics chromatin structure and antioxidant oxidant parameters after freeze thawing of hesperidin vitamin p doses added to ram semen |
| topic | semen hesperidin cryopreservation oxidative stress antioxidant ram |
| url | https://www.mdpi.com/2075-1729/12/11/1780 |
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