Detection of Klebsiella pneumoniae Carbapenemase (KPC) Producing Enterobacteriaceae Isolates from Various Clinical Samples in a Rural Health Setup
Carbapenem resistance is increasing and emerging as a public health threat. The Indian subcontinent serves as a reservoir for all 3 types of carbapenemases: KPC, OXA-181 and NDM. The present study was done to determine the antibiotic resistance pattern of Gram negative bacilli (GNB) belonging to...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2020-03-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/detection-of-klebsiella-pneumoniae-carbapenemase-kpc-producing-enterobacteriaceae-isolates-from-various-clinical-samples-in-a-rural-health-setup/ |
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| author | Arveen Sokhi Priti Agarwal Megha Maheshwari Anita Chakravarti |
| author_facet | Arveen Sokhi Priti Agarwal Megha Maheshwari Anita Chakravarti |
| author_sort | Arveen Sokhi |
| collection | DOAJ |
| description | Carbapenem resistance is increasing and emerging as a public health threat. The Indian subcontinent
serves as a reservoir for all 3 types of carbapenemases: KPC, OXA-181 and NDM. The present study
was done to determine the antibiotic resistance pattern of Gram negative bacilli (GNB) belonging to
family Enterobacteriaceae and molecular detection of blaKPC gene among the Carbapenem resistant
Enterobacteriaceae (CRE). Antibiotic sensitivity pattern of 301 gram negative isolates, members
of Enterobacteriaceae (E. coli, K. pneumoniae, K. oxytoca, Proteus species, Citrobacter species,
Enterobacter species and Serratia species) was determined and those resistant to carbapenem
(ertapenem) were further processed in the study. Modified Hodge Test (MHT) was performed to
phenotypically confirm the presence of carbapenemases (blaKPC) and Polymerase Chain Reaction (PCR)
was done to identify blaKPC gene. Of the total 301 isolates, 45 were resistant to ertapenem. Out of these
45 isolates 32 were MHT positive and three of these 32 MHT positive strains harboured blaKPC gene. |
| first_indexed | 2024-12-18T01:00:52Z |
| format | Article |
| id | doaj.art-b673ad6079f74ed0aa75270d3a4048cd |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-18T01:00:52Z |
| publishDate | 2020-03-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-b673ad6079f74ed0aa75270d3a4048cd2022-12-21T21:26:24ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2020-03-0114139740110.22207/JPAM.14.1.41Detection of Klebsiella pneumoniae Carbapenemase (KPC) Producing Enterobacteriaceae Isolates from Various Clinical Samples in a Rural Health SetupArveen Sokhi0https://orcid.org/0000-0002-7148-4132Priti Agarwal1Megha Maheshwari2https://orcid.org/0000-0003-2053-5902Anita Chakravarti3Department of Microbiology, FMHS, SGT University, Budhera, Gurugram- Badli road, Gurugram- 122505, Haryana, India.Department of Microbiology, FMHS, SGT University, Budhera, Gurugram- Badli road, Gurugram- 122505, Haryana, India.Department of Microbiology, Dr. Baba Saheb Ambedkar Medical College and Hospital, Sector-6, Rohini, New Delhi-110085, India.Department of Microbiology, FMHS, SGT University, Budhera, Gurugram- Badli road, Gurugram- 122505, Haryana, India.Carbapenem resistance is increasing and emerging as a public health threat. The Indian subcontinent serves as a reservoir for all 3 types of carbapenemases: KPC, OXA-181 and NDM. The present study was done to determine the antibiotic resistance pattern of Gram negative bacilli (GNB) belonging to family Enterobacteriaceae and molecular detection of blaKPC gene among the Carbapenem resistant Enterobacteriaceae (CRE). Antibiotic sensitivity pattern of 301 gram negative isolates, members of Enterobacteriaceae (E. coli, K. pneumoniae, K. oxytoca, Proteus species, Citrobacter species, Enterobacter species and Serratia species) was determined and those resistant to carbapenem (ertapenem) were further processed in the study. Modified Hodge Test (MHT) was performed to phenotypically confirm the presence of carbapenemases (blaKPC) and Polymerase Chain Reaction (PCR) was done to identify blaKPC gene. Of the total 301 isolates, 45 were resistant to ertapenem. Out of these 45 isolates 32 were MHT positive and three of these 32 MHT positive strains harboured blaKPC gene.https://microbiologyjournal.org/detection-of-klebsiella-pneumoniae-carbapenemase-kpc-producing-enterobacteriaceae-isolates-from-various-clinical-samples-in-a-rural-health-setup/blakpcenterobacteriaceaegram negative bacillicarbapenem resistancemodified hodge test |
| spellingShingle | Arveen Sokhi Priti Agarwal Megha Maheshwari Anita Chakravarti Detection of Klebsiella pneumoniae Carbapenemase (KPC) Producing Enterobacteriaceae Isolates from Various Clinical Samples in a Rural Health Setup Journal of Pure and Applied Microbiology blakpc enterobacteriaceae gram negative bacilli carbapenem resistance modified hodge test |
| title | Detection of Klebsiella pneumoniae Carbapenemase (KPC) Producing Enterobacteriaceae Isolates from Various Clinical Samples in a Rural Health Setup |
| title_full | Detection of Klebsiella pneumoniae Carbapenemase (KPC) Producing Enterobacteriaceae Isolates from Various Clinical Samples in a Rural Health Setup |
| title_fullStr | Detection of Klebsiella pneumoniae Carbapenemase (KPC) Producing Enterobacteriaceae Isolates from Various Clinical Samples in a Rural Health Setup |
| title_full_unstemmed | Detection of Klebsiella pneumoniae Carbapenemase (KPC) Producing Enterobacteriaceae Isolates from Various Clinical Samples in a Rural Health Setup |
| title_short | Detection of Klebsiella pneumoniae Carbapenemase (KPC) Producing Enterobacteriaceae Isolates from Various Clinical Samples in a Rural Health Setup |
| title_sort | detection of klebsiella pneumoniae carbapenemase kpc producing enterobacteriaceae isolates from various clinical samples in a rural health setup |
| topic | blakpc enterobacteriaceae gram negative bacilli carbapenem resistance modified hodge test |
| url | https://microbiologyjournal.org/detection-of-klebsiella-pneumoniae-carbapenemase-kpc-producing-enterobacteriaceae-isolates-from-various-clinical-samples-in-a-rural-health-setup/ |
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