The emergence of carbapenem-resistance and New Delhi metallo-β-lactamase-1 (blaNDM-1) among Salmonella spp. in Kerman, Iran
Background and Objectives: Salmonella species (spp) are the most prevalent zoonotic pathogens that cause outbreaks of gastroenteritis worldwide. Therefore evaluation of the profile of antibiotic resistance, virulence factors, and plasmid replicon types in these bacteria is necessary to control and...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2024-02-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/4282 |
| _version_ | 1827347990104244224 |
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| author | Sanaz Dehdashti Parvin Mohseni Reza Ghanbarpour Sajad Aslani Maryam-Sadat Moradiyan Davood Kalantar-Neyestanaki |
| author_facet | Sanaz Dehdashti Parvin Mohseni Reza Ghanbarpour Sajad Aslani Maryam-Sadat Moradiyan Davood Kalantar-Neyestanaki |
| author_sort | Sanaz Dehdashti |
| collection | DOAJ |
| description |
Background and Objectives: Salmonella species (spp) are the most prevalent zoonotic pathogens that cause outbreaks of gastroenteritis worldwide. Therefore evaluation of the profile of antibiotic resistance, virulence factors, and plasmid replicon types in these bacteria is necessary to control and prevent the spread of potentially pathogenic and drug-resistant strains.
Materials and Methods: This study was performed on 39 Salmonella spp. The antibacterial susceptibility of isolates to various antibiotic agents was determined using disk diffusion test. β-lactamases (bla) including ESBLs, AmpC, MBLs, and virulence genes were detected by PCR methods. Plasmid incompatibility groups among the isolates were identified using PCR-based replicon typing (PBRT).
Results: The most prevalent virulent gene was phoP/Q (84.6%). slyA, sopB, and stn were identified in 79.4% (n=31), 69.2% (n=27), and 2.5% (n=1) of the isolates, respectively. The antibiotic susceptibility testing showed that 30.7% of the isolates were ESBL-producing. blaTEM (41%; n=16) was the most frequent β-lactamase gene among the isolates followed by blaNDM-1 (15.4%; n=6), blaDHA (7.7%; n=3), and blaCTX-M (1.5%; n=1). Six different plasmid replicon types, including IncP (n=9; 23%), IncFIC (n=3; 7.70%), IncY (n=3; 7.70%), IncI1-Iγ (n=2; 5.12%), IncFIIAs (n=1; 2.56%), and IncN (n=1; 2.56%) were observed among the isolates.
Conclusion: Our study showed the emergence of carbapenem-resistant and blaNDM-1 among Salmonella spp. for the first time in Kerman, Iran. Since Salmonella spp. plays an important role in the transmission of resistance genes in livestock and humans in the food chains, so more stringent control policies are recommended to prevent the circulation of drug-resistant and potentially pathogenic strains from animals to humans.
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| first_indexed | 2024-03-08T00:02:20Z |
| format | Article |
| id | doaj.art-b674ac9763c34e20a35db5b8cba05ceb |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-03-08T00:02:20Z |
| publishDate | 2024-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-b674ac9763c34e20a35db5b8cba05ceb2024-02-18T04:04:22ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472024-02-0116110.18502/ijm.v16i1.14868The emergence of carbapenem-resistance and New Delhi metallo-β-lactamase-1 (blaNDM-1) among Salmonella spp. in Kerman, IranSanaz Dehdashti0Parvin Mohseni1Reza Ghanbarpour2Sajad Aslani3Maryam-Sadat Moradiyan4Davood Kalantar-Neyestanaki5Student Research Committee, Kerman University of Medical Sciences, Kerman, Iran; Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, IranDepartment of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, IranMolecular Microbiology Research Group, Shahid Bahonar University of Kerman, Kerman, IranDepartment of Pharmaceutics, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranStudent Research Committee, Kerman University of Medical Sciences, Kerman, IranMedical Mycology and Bacteriology Research Center, Kerman University of Medical Sciences, Kerman, Iran; Department of Medical Microbiology (Bacteriology and Virology), Afzalipour Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran Background and Objectives: Salmonella species (spp) are the most prevalent zoonotic pathogens that cause outbreaks of gastroenteritis worldwide. Therefore evaluation of the profile of antibiotic resistance, virulence factors, and plasmid replicon types in these bacteria is necessary to control and prevent the spread of potentially pathogenic and drug-resistant strains. Materials and Methods: This study was performed on 39 Salmonella spp. The antibacterial susceptibility of isolates to various antibiotic agents was determined using disk diffusion test. β-lactamases (bla) including ESBLs, AmpC, MBLs, and virulence genes were detected by PCR methods. Plasmid incompatibility groups among the isolates were identified using PCR-based replicon typing (PBRT). Results: The most prevalent virulent gene was phoP/Q (84.6%). slyA, sopB, and stn were identified in 79.4% (n=31), 69.2% (n=27), and 2.5% (n=1) of the isolates, respectively. The antibiotic susceptibility testing showed that 30.7% of the isolates were ESBL-producing. blaTEM (41%; n=16) was the most frequent β-lactamase gene among the isolates followed by blaNDM-1 (15.4%; n=6), blaDHA (7.7%; n=3), and blaCTX-M (1.5%; n=1). Six different plasmid replicon types, including IncP (n=9; 23%), IncFIC (n=3; 7.70%), IncY (n=3; 7.70%), IncI1-Iγ (n=2; 5.12%), IncFIIAs (n=1; 2.56%), and IncN (n=1; 2.56%) were observed among the isolates. Conclusion: Our study showed the emergence of carbapenem-resistant and blaNDM-1 among Salmonella spp. for the first time in Kerman, Iran. Since Salmonella spp. plays an important role in the transmission of resistance genes in livestock and humans in the food chains, so more stringent control policies are recommended to prevent the circulation of drug-resistant and potentially pathogenic strains from animals to humans. https://ijm.tums.ac.ir/index.php/ijm/article/view/4282Salmonella;Beta-lactamase genes;Virulence factors;New Delhi metallo-beta-lactamase-1 (NDM-1) |
| spellingShingle | Sanaz Dehdashti Parvin Mohseni Reza Ghanbarpour Sajad Aslani Maryam-Sadat Moradiyan Davood Kalantar-Neyestanaki The emergence of carbapenem-resistance and New Delhi metallo-β-lactamase-1 (blaNDM-1) among Salmonella spp. in Kerman, Iran Iranian Journal of Microbiology Salmonella; Beta-lactamase genes; Virulence factors; New Delhi metallo-beta-lactamase-1 (NDM-1) |
| title | The emergence of carbapenem-resistance and New Delhi metallo-β-lactamase-1 (blaNDM-1) among Salmonella spp. in Kerman, Iran |
| title_full | The emergence of carbapenem-resistance and New Delhi metallo-β-lactamase-1 (blaNDM-1) among Salmonella spp. in Kerman, Iran |
| title_fullStr | The emergence of carbapenem-resistance and New Delhi metallo-β-lactamase-1 (blaNDM-1) among Salmonella spp. in Kerman, Iran |
| title_full_unstemmed | The emergence of carbapenem-resistance and New Delhi metallo-β-lactamase-1 (blaNDM-1) among Salmonella spp. in Kerman, Iran |
| title_short | The emergence of carbapenem-resistance and New Delhi metallo-β-lactamase-1 (blaNDM-1) among Salmonella spp. in Kerman, Iran |
| title_sort | emergence of carbapenem resistance and new delhi metallo β lactamase 1 blandm 1 among salmonella spp in kerman iran |
| topic | Salmonella; Beta-lactamase genes; Virulence factors; New Delhi metallo-beta-lactamase-1 (NDM-1) |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/4282 |
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