Future Prospects of Luminescent Silicon Nanowires Biosensors
In this paper, we exploit the perspective of luminescent Si nanowires (NWs) in the growing field of commercial biosensing nanodevices for the selective recognition of proteins and pathogen genomes. We fabricated quantum confined fractal arrays of Si NWs with room temperature emission at 700 nm obtai...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/11/1052 |
| _version_ | 1827644863628181504 |
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| author | Maria Josè Lo Faro Antonio Alessio Leonardi Francesco Priolo Barbara Fazio Alessia Irrera |
| author_facet | Maria Josè Lo Faro Antonio Alessio Leonardi Francesco Priolo Barbara Fazio Alessia Irrera |
| author_sort | Maria Josè Lo Faro |
| collection | DOAJ |
| description | In this paper, we exploit the perspective of luminescent Si nanowires (NWs) in the growing field of commercial biosensing nanodevices for the selective recognition of proteins and pathogen genomes. We fabricated quantum confined fractal arrays of Si NWs with room temperature emission at 700 nm obtained by thin-film, metal-assisted, chemical etching with high production output at low cost. The fascinating optical features arising from multiple scattering and weak localization of light promote the use of Si NWs as optical biosensing platforms with high sensitivity and selectivity. In this work, label-free Si NW optical sensors are surface modified for the selective detection of C-reactive protein through antigen–gene interaction. In this case, we report the lowest limit of detection (LOD) of 1.6 fM, fostering the flexibility of different dynamic ranges for detection either in saliva or for serum analyses. By varying the NW surface functionalization with the specific antigen, the luminescence quenching of NW biosensors is used to measure the hepatitis B-virus pathogen genome without PCR-amplification, with an LOD of about 20 copies in real samples or blood matrix. The promising results show that NW optical biosensors can detect and isolate extracellular vesicles (EV) marked with CD81 protein with unprecedented sensitivity (LOD 2 × 10<sup>5</sup> sEV/mL), thus enabling their measurement even in a small amount of blastocoel fluid. |
| first_indexed | 2024-03-09T18:27:41Z |
| format | Article |
| id | doaj.art-b674b41bad6d4fbfb8bcc073ec04b302 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T18:27:41Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-b674b41bad6d4fbfb8bcc073ec04b3022023-11-24T07:49:09ZengMDPI AGBiosensors2079-63742022-11-011211105210.3390/bios12111052Future Prospects of Luminescent Silicon Nanowires BiosensorsMaria Josè Lo Faro0Antonio Alessio Leonardi1Francesco Priolo2Barbara Fazio3Alessia Irrera4Department of Physics and Astronomy, University of Catania, Via Santa Sofia 64, 95123 Catania, ItalyDepartment of Physics and Astronomy, University of Catania, Via Santa Sofia 64, 95123 Catania, ItalyDepartment of Physics and Astronomy, University of Catania, Via Santa Sofia 64, 95123 Catania, ItalyURT LAB SENS, Beyond Nano—CNR, c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno d’Alcontres 5, 98166 Messina, ItalyURT LAB SENS, Beyond Nano—CNR, c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno d’Alcontres 5, 98166 Messina, ItalyIn this paper, we exploit the perspective of luminescent Si nanowires (NWs) in the growing field of commercial biosensing nanodevices for the selective recognition of proteins and pathogen genomes. We fabricated quantum confined fractal arrays of Si NWs with room temperature emission at 700 nm obtained by thin-film, metal-assisted, chemical etching with high production output at low cost. The fascinating optical features arising from multiple scattering and weak localization of light promote the use of Si NWs as optical biosensing platforms with high sensitivity and selectivity. In this work, label-free Si NW optical sensors are surface modified for the selective detection of C-reactive protein through antigen–gene interaction. In this case, we report the lowest limit of detection (LOD) of 1.6 fM, fostering the flexibility of different dynamic ranges for detection either in saliva or for serum analyses. By varying the NW surface functionalization with the specific antigen, the luminescence quenching of NW biosensors is used to measure the hepatitis B-virus pathogen genome without PCR-amplification, with an LOD of about 20 copies in real samples or blood matrix. The promising results show that NW optical biosensors can detect and isolate extracellular vesicles (EV) marked with CD81 protein with unprecedented sensitivity (LOD 2 × 10<sup>5</sup> sEV/mL), thus enabling their measurement even in a small amount of blastocoel fluid.https://www.mdpi.com/2079-6374/12/11/1052silicon nanowiremetal assisted chemical etchingluminescenceoptical biosensorsproteinsgenome |
| spellingShingle | Maria Josè Lo Faro Antonio Alessio Leonardi Francesco Priolo Barbara Fazio Alessia Irrera Future Prospects of Luminescent Silicon Nanowires Biosensors Biosensors silicon nanowire metal assisted chemical etching luminescence optical biosensors proteins genome |
| title | Future Prospects of Luminescent Silicon Nanowires Biosensors |
| title_full | Future Prospects of Luminescent Silicon Nanowires Biosensors |
| title_fullStr | Future Prospects of Luminescent Silicon Nanowires Biosensors |
| title_full_unstemmed | Future Prospects of Luminescent Silicon Nanowires Biosensors |
| title_short | Future Prospects of Luminescent Silicon Nanowires Biosensors |
| title_sort | future prospects of luminescent silicon nanowires biosensors |
| topic | silicon nanowire metal assisted chemical etching luminescence optical biosensors proteins genome |
| url | https://www.mdpi.com/2079-6374/12/11/1052 |
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