Matrine regulates Th1/Th2 inflammatory responses by inhibiting the Hsp90/NF‐κB signaling axis to alleviate atopic dermatitis
Abstract Atopic dermatitis (AD) is a common inflammatory skin disease. Matrine is the main component of the traditional Chinese medicine Sophora flavescens, and it poses good therapeutic effects on inflammatory diseases. This study aimed to explore the pharmacological effects of matrine on AD and it...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-05-01
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| Series: | Kaohsiung Journal of Medical Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/kjm2.12655 |
| _version_ | 1827952066040954880 |
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| author | Pan Huang Fan Hu Zhi‐Bo Yang Yi Pan Rong Zhou Yi‐Ning Yan Hai‐Zhen Wang Chang Wang |
| author_facet | Pan Huang Fan Hu Zhi‐Bo Yang Yi Pan Rong Zhou Yi‐Ning Yan Hai‐Zhen Wang Chang Wang |
| author_sort | Pan Huang |
| collection | DOAJ |
| description | Abstract Atopic dermatitis (AD) is a common inflammatory skin disease. Matrine is the main component of the traditional Chinese medicine Sophora flavescens, and it poses good therapeutic effects on inflammatory diseases. This study aimed to explore the pharmacological effects of matrine on AD and its underlying mechanism. An AD mouse model and inflamed human epidermal keratinocyte cells (HaCaT) cells were established. Histopathological aspects were examined using hematoxylin and eosin staining, toluidine blue staining, and immunohistochemistry. The mRNA and protein expressions were assessed using quantitative real‐time polymerase chain reaction and Western blot, respectively. The secretions of cytokines and chemokines were examined by enzyme‐linked immunosorbent assay. Flow cytometry was carried out to analyze the proportions of T‐helper (Th) 1 and Th2 cells. Herein, our results displayed that matrine diminished AD symptoms and decreased heat shock protein 90 (Hsp90) expression. Matrine decreased the Th2 cytokine levels in the ear tissues and serum, and it also significantly repressed inflammatory cytokines (thymus activation regulated chemokine and interleukin‐6) secretions by repressing the Hsp90/NF‐κB signaling axis in inflamed HaCaT cells. Furthermore, matrine inhibited Th2 differentiation of CD4+ T cells when co‐cultured with inflamed HaCaT cells. Matrine can regulate the Th1/Th2 inflammatory response by inhibiting the Hsp90/NF‐κB signaling axis to alleviate AD. Therefore, it may be a candidate for AD treatment. |
| first_indexed | 2024-04-09T13:51:46Z |
| format | Article |
| id | doaj.art-b67612e165084f3b917393d54e98fd8e |
| institution | Directory Open Access Journal |
| issn | 1607-551X 2410-8650 |
| language | English |
| last_indexed | 2024-04-09T13:51:46Z |
| publishDate | 2023-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-b67612e165084f3b917393d54e98fd8e2023-05-08T13:45:36ZengWileyKaohsiung Journal of Medical Sciences1607-551X2410-86502023-05-0139550151010.1002/kjm2.12655Matrine regulates Th1/Th2 inflammatory responses by inhibiting the Hsp90/NF‐κB signaling axis to alleviate atopic dermatitisPan Huang0Fan Hu1Zhi‐Bo Yang2Yi Pan3Rong Zhou4Yi‐Ning Yan5Hai‐Zhen Wang6Chang Wang7Department of Dermatology Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine Changsha Hunan Province ChinaDepartment of anorectal Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine Changsha Hunan Province ChinaDepartment of Dermatology Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine Changsha Hunan Province ChinaDepartment of Dermatology Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine Changsha Hunan Province ChinaDepartment of Dermatology Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine Changsha Hunan Province ChinaDepartment of Dermatology Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine Changsha Hunan Province ChinaDepartment of Dermatology Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine Changsha Hunan Province ChinaDepartment of Dermatology Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine Changsha Hunan Province ChinaAbstract Atopic dermatitis (AD) is a common inflammatory skin disease. Matrine is the main component of the traditional Chinese medicine Sophora flavescens, and it poses good therapeutic effects on inflammatory diseases. This study aimed to explore the pharmacological effects of matrine on AD and its underlying mechanism. An AD mouse model and inflamed human epidermal keratinocyte cells (HaCaT) cells were established. Histopathological aspects were examined using hematoxylin and eosin staining, toluidine blue staining, and immunohistochemistry. The mRNA and protein expressions were assessed using quantitative real‐time polymerase chain reaction and Western blot, respectively. The secretions of cytokines and chemokines were examined by enzyme‐linked immunosorbent assay. Flow cytometry was carried out to analyze the proportions of T‐helper (Th) 1 and Th2 cells. Herein, our results displayed that matrine diminished AD symptoms and decreased heat shock protein 90 (Hsp90) expression. Matrine decreased the Th2 cytokine levels in the ear tissues and serum, and it also significantly repressed inflammatory cytokines (thymus activation regulated chemokine and interleukin‐6) secretions by repressing the Hsp90/NF‐κB signaling axis in inflamed HaCaT cells. Furthermore, matrine inhibited Th2 differentiation of CD4+ T cells when co‐cultured with inflamed HaCaT cells. Matrine can regulate the Th1/Th2 inflammatory response by inhibiting the Hsp90/NF‐κB signaling axis to alleviate AD. Therefore, it may be a candidate for AD treatment.https://doi.org/10.1002/kjm2.12655atopic dermatitisHsp90keratinocytesmatrineTh1/Th2 inflammatory response |
| spellingShingle | Pan Huang Fan Hu Zhi‐Bo Yang Yi Pan Rong Zhou Yi‐Ning Yan Hai‐Zhen Wang Chang Wang Matrine regulates Th1/Th2 inflammatory responses by inhibiting the Hsp90/NF‐κB signaling axis to alleviate atopic dermatitis Kaohsiung Journal of Medical Sciences atopic dermatitis Hsp90 keratinocytes matrine Th1/Th2 inflammatory response |
| title | Matrine regulates Th1/Th2 inflammatory responses by inhibiting the Hsp90/NF‐κB signaling axis to alleviate atopic dermatitis |
| title_full | Matrine regulates Th1/Th2 inflammatory responses by inhibiting the Hsp90/NF‐κB signaling axis to alleviate atopic dermatitis |
| title_fullStr | Matrine regulates Th1/Th2 inflammatory responses by inhibiting the Hsp90/NF‐κB signaling axis to alleviate atopic dermatitis |
| title_full_unstemmed | Matrine regulates Th1/Th2 inflammatory responses by inhibiting the Hsp90/NF‐κB signaling axis to alleviate atopic dermatitis |
| title_short | Matrine regulates Th1/Th2 inflammatory responses by inhibiting the Hsp90/NF‐κB signaling axis to alleviate atopic dermatitis |
| title_sort | matrine regulates th1 th2 inflammatory responses by inhibiting the hsp90 nf κb signaling axis to alleviate atopic dermatitis |
| topic | atopic dermatitis Hsp90 keratinocytes matrine Th1/Th2 inflammatory response |
| url | https://doi.org/10.1002/kjm2.12655 |
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