Drinking water disinfection byproduct iodoacetic acid affects thyroid hormone synthesis in Nthy-ori 3–1 cells
Iodoacetic acid (IAA) is an emerging and the most genotoxic iodinated disinfection byproduct to date. IAA can disrupt the thyroid endocrine function in vivo and in vitro, but the underlying mechanisms remain unclear. In this work, transcriptome sequencing was used to investigate the effect of IAA on...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S014765132300430X |
| _version_ | 1797833148463054848 |
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| author | Jingyi Xiao Yujie Sha Yuwen Huang Kunling Long Huan Wu Yan Mo Qiyuan Yang Shengkun Dong Qiang Zeng Xiao Wei |
| author_facet | Jingyi Xiao Yujie Sha Yuwen Huang Kunling Long Huan Wu Yan Mo Qiyuan Yang Shengkun Dong Qiang Zeng Xiao Wei |
| author_sort | Jingyi Xiao |
| collection | DOAJ |
| description | Iodoacetic acid (IAA) is an emerging and the most genotoxic iodinated disinfection byproduct to date. IAA can disrupt the thyroid endocrine function in vivo and in vitro, but the underlying mechanisms remain unclear. In this work, transcriptome sequencing was used to investigate the effect of IAA on the cellular pathways of human thyroid follicular epithelial cell line Nthy-ori 3–1 and determine the mechanism of IAA on the synthesis and secretion of thyroid hormone (TH) in Nthy-ori 3–1 cells. Results of transcriptome sequencing indicated that IAA affected the TH synthesis pathway in Nthy-ori 3–1 cells. IAA reduced the mRNA expression of thyroid stimulating hormone receptor, sodium iodide symporter, thyroid peroxidase, thyroglobulin, paired box 8 and thyroid transcription factor-2, inhibited the cAMP/PKA pathway and Na+-K+-ATPase, and decreased the iodine intake. The results were confirmed by our previous findings in vivo. Additionally, IAA downregulated glutathione and the mRNA expression of glutathione peroxidase 1, leading to increased reactive oxygen species production. This study is the first to elucidate the mechanisms of IAA on TH synthesis in vitro. The mechanisms are associated with down-regulating the expression of genes related to TH synthesis, inhibiting iodine uptake, and inducing oxidative stress. These findings may improve future health risk assessment of IAA on thyroid in human. |
| first_indexed | 2024-04-09T14:19:54Z |
| format | Article |
| id | doaj.art-b676de1364a54683adcd8d3d9f7a2f23 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-09T14:19:54Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-b676de1364a54683adcd8d3d9f7a2f232023-05-05T04:39:39ZengElsevierEcotoxicology and Environmental Safety0147-65132023-06-01257114926Drinking water disinfection byproduct iodoacetic acid affects thyroid hormone synthesis in Nthy-ori 3–1 cellsJingyi Xiao0Yujie Sha1Yuwen Huang2Kunling Long3Huan Wu4Yan Mo5Qiyuan Yang6Shengkun Dong7Qiang Zeng8Xiao Wei9Department of Occupational and Environmental Health, School of Public Health, Guangxi Medical University, Nanning, Guangxi 530021, ChinaDepartment of Occupational and Environmental Health, School of Public Health, Guangxi Medical University, Nanning, Guangxi 530021, ChinaDepartment of Occupational and Environmental Health, School of Public Health, Guangxi Medical University, Nanning, Guangxi 530021, ChinaDepartment of Occupational and Environmental Health, School of Public Health, Guangxi Medical University, Nanning, Guangxi 530021, ChinaDepartment of Occupational and Environmental Health, School of Public Health, Guangxi Medical University, Nanning, Guangxi 530021, ChinaDepartment of Occupational and Environmental Health, School of Public Health, Guangxi Medical University, Nanning, Guangxi 530021, ChinaDepartment of Occupational and Environmental Health, School of Public Health, Guangxi Medical University, Nanning, Guangxi 530021, ChinaSouthern Laboratory of Ocean Science and Engineering, School of Civil Engineering, Sun Yat-Sen University, Zhuhai 519000, ChinaDepartment of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, ChinaDepartment of Occupational and Environmental Health, School of Public Health, Guangxi Medical University, Nanning, Guangxi 530021, China; Key Laboratory of Longevity and Aging-related Diseases of Chinese Ministry of Education, Guangxi Medical University, Nanning, Guangxi 530021, China; Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases, School of Public Health, Guangxi Medical University, Nanning, Guangxi 530021, China; Guangxi Key Laboratory of Environment and Health Research, Guangxi Medical University, Nanning, Guangxi 530021, China; Corresponding author at: Department of Occupational and Environmental Health, School of Public Health, Guangxi Medical University, Nanning, Guangxi 530021, China.Iodoacetic acid (IAA) is an emerging and the most genotoxic iodinated disinfection byproduct to date. IAA can disrupt the thyroid endocrine function in vivo and in vitro, but the underlying mechanisms remain unclear. In this work, transcriptome sequencing was used to investigate the effect of IAA on the cellular pathways of human thyroid follicular epithelial cell line Nthy-ori 3–1 and determine the mechanism of IAA on the synthesis and secretion of thyroid hormone (TH) in Nthy-ori 3–1 cells. Results of transcriptome sequencing indicated that IAA affected the TH synthesis pathway in Nthy-ori 3–1 cells. IAA reduced the mRNA expression of thyroid stimulating hormone receptor, sodium iodide symporter, thyroid peroxidase, thyroglobulin, paired box 8 and thyroid transcription factor-2, inhibited the cAMP/PKA pathway and Na+-K+-ATPase, and decreased the iodine intake. The results were confirmed by our previous findings in vivo. Additionally, IAA downregulated glutathione and the mRNA expression of glutathione peroxidase 1, leading to increased reactive oxygen species production. This study is the first to elucidate the mechanisms of IAA on TH synthesis in vitro. The mechanisms are associated with down-regulating the expression of genes related to TH synthesis, inhibiting iodine uptake, and inducing oxidative stress. These findings may improve future health risk assessment of IAA on thyroid in human.http://www.sciencedirect.com/science/article/pii/S014765132300430XDrinking waterDisinfection byproductsIodoacetic acidThyroid hormoneIodine uptakeOxidative stress |
| spellingShingle | Jingyi Xiao Yujie Sha Yuwen Huang Kunling Long Huan Wu Yan Mo Qiyuan Yang Shengkun Dong Qiang Zeng Xiao Wei Drinking water disinfection byproduct iodoacetic acid affects thyroid hormone synthesis in Nthy-ori 3–1 cells Ecotoxicology and Environmental Safety Drinking water Disinfection byproducts Iodoacetic acid Thyroid hormone Iodine uptake Oxidative stress |
| title | Drinking water disinfection byproduct iodoacetic acid affects thyroid hormone synthesis in Nthy-ori 3–1 cells |
| title_full | Drinking water disinfection byproduct iodoacetic acid affects thyroid hormone synthesis in Nthy-ori 3–1 cells |
| title_fullStr | Drinking water disinfection byproduct iodoacetic acid affects thyroid hormone synthesis in Nthy-ori 3–1 cells |
| title_full_unstemmed | Drinking water disinfection byproduct iodoacetic acid affects thyroid hormone synthesis in Nthy-ori 3–1 cells |
| title_short | Drinking water disinfection byproduct iodoacetic acid affects thyroid hormone synthesis in Nthy-ori 3–1 cells |
| title_sort | drinking water disinfection byproduct iodoacetic acid affects thyroid hormone synthesis in nthy ori 3 1 cells |
| topic | Drinking water Disinfection byproducts Iodoacetic acid Thyroid hormone Iodine uptake Oxidative stress |
| url | http://www.sciencedirect.com/science/article/pii/S014765132300430X |
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